The Effect of Cholesterol Addition, Buffer, and pH on Equine Sperm Stored at 5°C

Crespilho, André Maciel; Spizziri, B.; Meyers, M.; Graham, James K.

doi:10.1016/j.jevs.2012.09.004

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…Lower storage temperature decreases metabolic rate, slows chemical reactions and reduces bacterial growth, extending the life of fertile spermatozoa [26]. In one research with stallion sperm, pH was not a significant source of variation in motility and live sperm percentage over different cooling periods [6]. In our study, samples with higher motility had lower pH.…”

Section: Discussioncontrasting

confidence: 41%

“…The maintenance of sperm viability for at least 24 h is essential in the equine reproduction [31]; in Brazil, however, considering distance and logistics, this period should be extended to 48 h. It has already been proven that there is a decrease in motility, membrane integrity [24] and fertilizing ability [22] of equine spermatozoa during cooled-storage. Also occur during cooled storage, the "cold shock" [5,9] and other changes such as lipid peroxidation [14], modifications in pH [6], ATP depletion [7], oxidative phosphorylation [27], and bacterial contamination [30].…”

Section: Introductionmentioning

confidence: 99%

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Viability of Pony Stallion Semen in Different Temperature and Dilution

Trentin

Rodrigues

Pessoa

et al. 2017

Acta Scientiae. Vet.

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Background: Artificial insemination and transport of cooled semen has been routinely used in equine industry in the past 20 years. However, more investigations are needed regarding the methods for long time storage in pony stallion semen. The effect of dilution and cooling temperature on pH, sperm motility, membrane integrity and mitochondrial activity were investigated before and after cooling of stallion semen.Materials, Methods & Results: Two ejaculates each from nine Brazilian ponies were diluted in a nonbuffered powder milk extender cooled at 5°C or 15°C for 48 h using three different dilutions (1:1, 1:2 or 1:3). Data were assessed by analysis of variance and the rate comparison was performed using the Duncan test. Samples diluted 1:1 at 5oC or 15°C showed higher pH values (7.63 ± 0.34 e 7.57 ± 0.27) and lower progressive motility (10.3 ± 11.05, 17.08 ± 9.95). All samples cooled at 15°C also showed lower incidence of morphologically altered spermatozoa (1:1 = 55.84%; 1:2 = 51.84%; 1:3 = 49.95%) [P < 0.01]. Mitochondrial activity was higher on the 1:3 dilution (0.86 ± 0.19 nm) at 5°C and on the 1:1 (0.89 ± 0.23 nm), 1:2 (0.93 ± 0.2 nm) and 1:3 (0.92 ± 0.2 nm) dilutions at 15°C. Progressive motility was higher when semen was diluted 1:3 and cooled at 15°C (42.22 ± 12.38; P < 0.05). Considering mitochondrial activity, similar results were observed when different dilutions of semen were used (P > 0.05) despite time and temperature. The pH, progressive motility, mitochondrial activity and membrane integrity remained similar (P > 0.05) on fresh semen samples independent of the dilution grade used. The best results were obtained when semen was diluted 1:3 and cooled at 15°C. All dilution grades were safe for fresh semen and pH wasincreased when semen was diluted and cooled for 48 h.Discussion: The methodology used to collect and process equine semen and semen from ponies is practically the same. Equine semen when sent for artificial insemination is usually cooled to 5°C. Our results showed that cooling reduces sperm viability, which has also been demonstrated by other studies. In contrast, the best cooling temperature was at 15°C. However, it is easier to keep the temperature at 5°C during transport, due to the large temperature oscillation that may occur during transportation. The semen of ponies can tolerate cooling at both 5 and 15°C. The 1:3 dilution cooled to 15°C provided better viability of pony sperm, and more stable pH during 48 h of cooling. Dilution 1:1 should not be used for cooling in powdered skim milk extender.

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Section: Discussioncontrasting

confidence: 41%

Section: Introductionmentioning

confidence: 99%

Viability of Pony Stallion Semen in Different Temperature and Dilution

Trentin

Rodrigues

Pessoa

et al. 2017

Acta Scientiae. Vet.

View full text Add to dashboard Cite

show abstract

“…These results suggest a better incorporation of cholesterol into the sperm membrane with time offering them a better protection. Crespilho et al [8] showed that increasing the incubation time till 24 h during the storage of equine sperm at 5 C improved the equine motility and survival but long incubation time with cholesterol may affect capacitation process.…”

Section: Discussionmentioning

confidence: 99%