This research investigated the viability of using Hibiscus Sabdarifa sepal mucilage (HSSM) in conjunction with glycerol (10, 15 and 20%, w/w) and carboxymethyl cellulose (1%, w/w) to create a new biodegradable edible film. The microstructural, thermal, mechanical, physical, and antioxidant activities of the films made for use in the packaging sector were tested and assessed. Overall, the results demonstrated that increasing the percentage of glycerol in the optimal amount of Hibiscus Sabdarifa sepal mucilage increased the film thickness, moisture content, solubility, water vapor permeability, and elongation at break (EB%). Significant reductions were seen in the tensile strength, melting point, and glass transition temperature (Tg). The color of the films indicated that, despite becoming faintly green or crimson as the glycerol concentration increased, they remained transparent. In addition, the produced film exhibited notable antioxidant properties. Compared to films without glycerol, glycerol-containing film samples exhibited a uniform surface morphology devoid of cavities and fractures in electron microscope images. In combination with carboxymethyl cellulose, the sepal mucilage of Hibiscus Sabdarifa, a novel and innovative source, led to the production of biodegradable edible films with desirable properties.