The Effect of Chaenomelis Fructus Extract on Thioacetamide-Induced Liver Fibrosis

Abstract

This study was conducted to evaluate the effect of Chaenomelis Fructus (CF) extract on thioacetamide (TAA)-induced liver fibrosis in mice. The mice were divided into four groups for examination: Normal group (Normal, n=9), group with liver fibrosis administered distilled water (Control, n=9), group with liver fibrosis administered silymarin 100 mg/kg (Silymarin, n=9), group with liver fibrosis administered CF 200 mg/kg (CF, n=9). Liver fibrosis was established in the mice via an injection of TAA (1 week 100 mg/kg B.W., 2∼3 weeks 200 mg/kg B.W., 4∼8 weeks 400 mg/kg B.W., three times a week, I.P) for 8 weeks and they were administered silymarin and CF extract (every day, P.O) with the TAA. After the autopsy, we analyzed the expression of inflammation-related proteins and fibrosis-related factors in the liver tissue by the western blot test. The results of the experiment showed that the administration of CF regulated the expression of phosphorylated AMPK (p-AMPK) and Sirtuin 1 (SIRT1) and inhibited the expression of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. In addition, the expressions of matrix metalloproteinase-2 (MMP-2), TIMP metallopeptidase inhibitor 1 (TIMP-1), ɑ-smooth muscle actin (ɑ-SMA), and collagen Ⅰ related to fibrosis were suppressed. These results suggest that the progression of liver fibrosis may be alleviated by regulating the AMPK/SIRT1/NF-κB pathway, as well as the expression of fibrosis-related factors.

Study on Chemical Composition and Biological Activity of Psidium guajava Leaf Extracts

“…These results align with the findings of Hong et al [ 27 ], where the extract yields increased in the order of 70% ethanol < 50% ethanol < 30% ethanol, indicating a tendency for higher yields with greater solvent polarity. This trend is consistent with the study on the antioxidant effects of quince ethanol extract by Lee et al [ 28 ], where the yields of the fractions as a function of solvent also exhibited a pattern of higher yields with increasing solvent polarity. Since ethanol is a non-polar solvent compared to water, it is believed that the difference in polarity between the solvents leads to variations in the composition of the eluted substances.…”

Liver Protective Effect of the Co-treatment of Rhei Radix et Rhizoma and Silymarin on TAA-induced Liver Injury

“…기저막의 손상은 간성상세 포를 활성화하고 근섬유모세포(myofibroblast)형태 희 세포로 변하여 a-SMA와 같은 단백질 표현형을 분화 및 활성화시킨다 3 . 최근에는 HSC의 활성화 단계의 치료를 기반으로 연구가 진행되고 있으며, 여러 간손상 모델에서 독성 대사물로 인한 산화적 스트레스의 생성을 억제하고, ECM에 중요한 역할 을 하는 간손상 개선을 위한 약재나 식품에 사용 되는 천연물 소재를 찾는데 관심이 많아지는 추세 이다 4,5 .…”