The effect of cellular age on zinc levels in untreated and zinc-treated human diploid fibroblasts

Monticone, Robert E.; Nick, Robert; Eichhorn, G.

doi:10.1016/0162-0134(87)80072-7

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…(2 × 10 −29 ), in the extracts the concentration of zinc will be low (Balasubramanian, Strobel, Kneser, & Boccaccini, 2015). According to all these finding, the low cumulative Zn 2+ release in this study can promote bone formation and has no inhibition effect on cell proliferation and activation (Borovanský & Riley, 1989;Epstein, 1982;Monticone, Nick, & Eichhorn, 1987). some Streptococci, and some Gram-negative bacteria (Söderberg et al, 1990); however, mechanisms by which zinc inhibits or kills bacteria have not been clearly defined (Sugarman, 1983 (Sawai et al, 1996).…”

Section: Discussionmentioning

confidence: 65%

“…Due to the fact that the solubility constant of zinc phosphate is 9.1 × 10 −33 and therefore lower than the solubility constant of calcium phosphate (2 × 10 −29 ), in the extracts the concentration of zinc will be low (Balasubramanian, Strobel, Kneser, & Boccaccini, 2015). According to all these finding, the low cumulative Zn 2+ release in this study can promote bone formation and has no inhibition effect on cell proliferation and activation (Borovanský & Riley, 1989; Epstein, 1982; Monticone, Nick, & Eichhorn, 1987).…”

Section: Discussionmentioning

confidence: 73%

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In vitro evaluation of novel titania‐containing borate bioactive glass scaffolds

Shafaghi

Rodriguez

Wren

et al. 2020

J Biomedical Materials Res

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Titanium-containing borate bioactive glass scaffolds (0, 5, 15, and 20 mol %, identified as BRT0, BRT1, BRT3, and BRT4) with a microstructure similar to that of human trabecular bone were prepared and evaluated in vitro for potential bone loss applications in revision total knee arthroplasty (rTKA). Methyl thiazolyl tetrazolium (MTT) cell viability assays of scaffold ion release extracts revealed that BRT0 scaffolds (0 mol % titanium) inhibited cell proliferation and activity at day 14. At day 30, all scaffold extracts decreased cell proliferation and activity significantly. However, live/ dead cell assay results demonstrated that degradation products from all the scaffolds had no inhibitory effect on cell viability. Significant bactericidal efficacies of BRT3

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 73%

In vitro evaluation of novel titania‐containing borate bioactive glass scaffolds

Shafaghi

Rodriguez

Wren

et al. 2020

J Biomedical Materials Res

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“…This leads to generation of oxidative stress and thus high cytotoxicityin contrast to the redox-inert Zn(II) complexes. The phenomenon that Zn(II) can be more toxic than Cu(II) in fibroblast cells has been described before in the literature …”

Section: Resultsmentioning

confidence: 82%

“…The phenomenon that Zn(II) can be more toxic than Cu(II) in fibroblast cells has been described before in the literature. 54 To gain a better insight into the importance of cellular uptake for the cytotoxicity, Cu(II) L1 and Cu(II) L4 as two representative compounds with low and high cytotoxicity were incubated with A549 cells, and the Cu(II) content was determined by atomic absorption spectroscopy (AAS). Whereas only 0.07 nmol Cu/mg protein was detected for Cu(II) L1 after 24 h, it was almost 40 times as much for Cu(II) L4 (cf.…”

Section: ■ Introductionmentioning

confidence: 99%

Multiply Intercalator-Substituted Cu(II) Cyclen Complexes as DNA Condensers and DNA/RNA Synthesis Inhibitors

et al. 2018

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Many drugs that are applied in anticancer therapy such as the anthracycline doxorubicin contain DNA-intercalating 9,10-anthraquinone (AQ) moieties. When Cu(II) cyclen complexes were functionalized with up to three (2-anthraquinonyl)methyl substituents, they efficiently inhibited DNA and RNA synthesis resulting in high cytotoxicity (selective for cancer cells) accompanied by DNA condensation/aggregation phenomena. Molecular modeling suggests an unusual bisintercalation mode with only one base pair between the two AQ moieties and the metal complex as a linker. A regioisomer, in which the AQ moieties point in directions unfavorable for such an interaction, had a much weaker biological activity. The ligands alone and corresponding Zn(II) complexes (used as redox inert control compounds) also exhibited lower activity.

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“…36 This latter repeating disaccharide has also been reported in diabetic kidney. 37 Altered levels of Zn are a feature of ageing 38 and neurodegenerative disease, 39 while GAG expression is also known to vary 40 and both potassium and calcium levels have been related to ageing in Drosophila. 41 The levels of cations and expression of HS structures both vary considerably during normal and disease processes and here, the detection of GAG structures using phage display antibodies is affected by cation levels.…”

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confidence: 99%

Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding

et al. 2015

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a Phage display antibodies are widely used to follow heparan sulfate (HS) expression in tissues and cells.We demonstrate by ELISA, that cations alter phage display antibody binding profiles to HS and this is mediated by changes in polysaccharide conformation, demonstrated by circular dichroism spectroscopy. Native HS structures, expressed on the cell surfaces of neuroblastoma and fibroblast cells, also exhibited altered antibody binding profiles following exposure to low mM concentrations of these cations. Phage display antibodies recognise conformationally-defined HS epitopes, rather than sequence alone, as has been assumed, and resemble proteins in being sensitive to changes in both charge distribution and conformation following binding of cations to HS polysaccharides.

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The effect of cellular age on zinc levels in untreated and zinc-treated human diploid fibroblasts

Cited by 8 publications

References 21 publications

In vitro evaluation of novel titania‐containing borate bioactive glass scaffolds

In vitro evaluation of novel titania‐containing borate bioactive glass scaffolds

Multiply Intercalator-Substituted Cu(II) Cyclen Complexes as DNA Condensers and DNA/RNA Synthesis Inhibitors

Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding

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