The effect of CD34 + cell telomere length and hTERT expression on the outcome of autologous CD34 + cell transplantation in patients with chronic heart failure

Rozman, Jasmina-Ziva; Perme, Maja Pohar; Jež, Mojca; Maličev, Elvira; Krasna, Metka; Novaković, Srdjan; Vrtovec, Bojan; Rožman, Primož

doi:10.1016/j.mad.2017.06.001

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…QPCR SYBR Green Master Mix was used to detect the expression levels of TERT. Gene expression was quanti ed relative to the housekeeping gene GAPDH using the 2 −ΔΔCT method [27]. The following primer sequences were used for the TERT and GAPDH: TERT-F, 5'-CTCCCATTTCATCAGCAAGTTT − 3' and TERT-R, 5'-CTTGGCTTTCAGGATGGAGTAG − 3', and for the housekeeping gene GAPDH-F, 5'-GGAAGCTTGTCATCAATGGAAATC-3' and GAPDH-R, 5'-TGATGACCCTTTTGGCTCCC − 3'.…”

Section: Tert Expression Measurement Of Gcmentioning

confidence: 99%

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Hao

Jing

et al. 2021

Preprint

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Background: Telomere attrition has been shown to play a critical role in the reproductive aging process in human beings. Telomere length (TL) is normally regulated by telomerase enzyme. Telomerase reverse transcriptase (TERT) is the main component of the telomerase. Anti-Mullerian hormone (AMH), a member of the transforming growth factor superfamily, is derived from the granulosa cells of early developing pre-antral and antral follicles. The aim of this study was to evaluate the associations between relative telomere length (RTL), TERT expression of granulosa cells (GC), follicular fluid (FF) AMH levels and ovarian/embryonic performance in infertile women at different age. Moreover, whether they acting as predictors for probability of clinical pregnancy were also assessed. Method: A total of 160 women underwent their ﬁrst fresh cycle of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) were included in our study as follows: 100 women were enrolled for RTL measurement and 60 women were enrolled for TERT measurement. All these 160 women underwent FF AMH measurement. Correlations between RTL，TERT expression, FF AMH levels and age, ovarian/embryonic performance and probability of clinical pregnancy were assessed.Results: There was a statistically significant relationship between the expression levels of TERT, RTL, FF AMH levels and patient age(r = −0.20, P = 0.04; r=0.30, P=0.02; r=-0.191, P=0.003, respectively). Relationships between the expression levels of TERT, FF AMH levels and oocytes yield were significant (P<0.001; P<0.05, respectively). However, no statistically correlation was observed between the RTL of GC samples and oocytes yield. All these three biomarkers had no correlation with blastocyst formation rate. There was significant relationship between FF AMH levels and probability of clinical pregnancy in patients older than 35 years (OR=1.284, 95%CI=1.031-1.599, P=0.026). Conclusion: RTL, relative TERT expression in GC and AMH levels in follicular fluid are age-related, but all of them fail to predict embryonic outcomes. Relative TERT expression and FF AMH levels appear to be more reliable for prediction of ovarian response than RTL. FF AMH is also a good predictor for probability of clinical pregnancy in advanced women.

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Section: Tert Expression Measurement Of Gcmentioning

confidence: 99%

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Hao

Jing

et al. 2021

Preprint

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“…The telomere length of autologous CD34 + cells did not have any influence on the clinical outcome in cardiomyopathy patients. However, a higher CD34 + human Telomerase reverse Transcriptase (hTERT) expression was associated with better clinical outcome [ 53 ]. A study found that the increasing age is associated with low peripheral blood CD34 + cells and diminished bone marrow stem cell mobilization by Granulocyte Colony Stimulating Factor (GCSF) in ischemic heart failure patients [ 54 ].…”

Section: Senescence and Its Effect On Stem Cell Biology Transplanmentioning

confidence: 99%

Nano-Vesicle (Mis)Communication in Senescence-Related Pathologies

Saheera

Potnuri²,

Krishnamurthy

2020

Cells

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Extracellular vesicles are a heterogeneous group of cell-derived membranous structures comprising of exosomes, apoptotic bodies, and microvesicles. Of the extracellular vesicles, exosomes are the most widely sorted and extensively explored for their contents and function. The size of the nanovesicular structures (exosomes) range from 30 to 140 nm and are present in various biological fluids such as saliva, plasma, urine etc. These cargo-laden extracellular vesicles arise from endosome-derived multivesicular bodies and are known to carry proteins and nucleic acids. Exosomes are involved in multiple physiological and pathological processes, including cellular senescence. Exosomes mediate signaling crosstalk and play a critical role in cell–cell communications. Exosomes have evolved as potential biomarkers for aging-related diseases. Aging, a physiological process, involves a progressive decline of function of organs with a loss of homeostasis and increasing probability of illness and death. The review focuses on the classic view of exosome biogenesis, biology, and age-associated changes. Owing to their ability to transport biological information among cells, the review also discusses the interplay of senescent cell-derived exosomes with the aging process, including the susceptibility of the aging population to COVID-19 infections.

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“…A recent clinical study using autologous cell transplantation of peripheral blood-derived CD34+ cells in 43 patients with cardiomyopathy highlighted that higher expression levels of TERT in cell therapeutics improved their therapeutic effect against cardiovascular illnesses [26], suggesting that potential TERT gene transfer by vectors is a promising strategy to enhance the efficacy of cell therapeutics. These findings illustrated that higher human TERT expression levels within CD34+ cells were associated with better clinical outcomes for patients suffering from cardiomyopathy following cell engraftment by transendocardial delivery.…”

Section: Regenerative and Anti-aging Applicationsmentioning

confidence: 99%

Telomere Gene Therapy: Polarizing Therapeutic Goals for Treatment of Various Diseases

Hong

Yun²

2019

Cells

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Modulation of telomerase maintenance by gene therapy must meet two polarizing requirements to achieve different therapeutic outcomes: Anti-aging/regenerative applications require upregulation, while anticancer applications necessitate suppression of various genes integral to telomere maintenance (e.g., telomerase, telomerase RNA components, and shelterin complex). Patients suffering from aging-associated illnesses often exhibit telomere attrition, which promotes chromosomal instability and cellular senescence, thus requiring the transfer of telomere maintenance-related genes to improve patient outcomes. However, reactivation and overexpression of telomerase are observed in 85% of cancer patients; this process is integral to cancer immortality. Thus, telomere-associated genes in the scope of cancer gene therapy must be inactivated or inhibited to induce anticancer effects. These contradicting requirements for achieving different therapeutic outcomes mean that any vector-mediated upregulation of telomere-associated genes must be accompanied by rigorous evaluation of potential oncogenesis. Thus, this review aims to discuss how telomere-associated genes are being targeted or utilized in various gene therapy applications and provides some insight into currently available safety hazard assessments.

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The effect of CD34 + cell telomere length and hTERT expression on the outcome of autologous CD34 + cell transplantation in patients with chronic heart failure

Cited by 5 publications

References 30 publications

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Nano-Vesicle (Mis)Communication in Senescence-Related Pathologies

Telomere Gene Therapy: Polarizing Therapeutic Goals for Treatment of Various Diseases

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