The effect of Caulerpa sertularioides extract on bull sperm freezablity and subsequent embryo development

Saberivand, Adel; Sarvarzadeh, Fatemeh; Peighambarzadeh, Seyedeh Zeinab; Saberivand, Maryam; Pakizehvand, Hajyeh; Rashidi, Sahar; Rahbar, Maryam; Khoshniyat, Mohammadrasoul

doi:10.1016/j.theriogenology.2022.06.017

Cited by 2 publications

(1 citation statement)

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“…To overcome the loss of semen quality during storage, several extracts from plants have been listed as natural antioxidants that could target such efects [32][33][34]. Even, to the best of our knowledge, there is no report in the literature on the supplementation of ram semen with ETHEX.…”

Section: Discussionmentioning

confidence: 99%

The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality

Allai

Druart

Terzioğlu

et al. 2023

Veterinary Medicine International

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Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context, the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH•) assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES) and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70% motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8% of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48, and 72 h. The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2% ETHEX in SM increased the sperm motility, viability, and membrane integrity and decreased the abnormality of spontaneous and catalyzed lipids peroxidation ( p < 0.05 ) up to 72 h. In addition, semen diluted with 1 and 2% ETHEX decreased the level of DNA fragmentation compared to the control group ( p < 0.05 ). In conclusion, the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on artificial insemination should be further studied.

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Section: Discussionmentioning

confidence: 99%