The Effect of Catalase Amplification on Immortal Lens Epithelial Cell Lines

Removal of Oxidatively Damaged Proteins from Lens Cells by the Ubiquitin-Proteasome Pathway

“…Because the lens epithelial cells have an active system to detoxify H 2 O 2 Yang et al, 1998;Reddan et al, 1999), we used a continuous H 2 O 2 -generating system to maintain constant H 2 O 2 . Consistent with previous reports, intracellular GSH levels decrease and GSSG levels increase upon exposure to H 2 O 2 .…”

Investigation of the Mechanism of Action of Microperoxidase-11, (MP11), a Potential Anti-cataract Agent, with Hydrogen Peroxide and Ascorbate

“…Furthermore, exposure of cultured rat lenses to H 2 O 2 at levels found in cataract patients causes cataract (Spector, Wang and Wang, 1993;Spector et al, 1997) and reaction of lens protein with H 2 O 2 and chelated iron produces changes in the protein which closely resemble modi®cations found in the cataractous lens (Zigler et al, 1985;Zigler, Huang and Du, 1989). Finally, in vitro studies have demonstrated that either catalase or a glutathione peroxidase mimic, both of which can degrade H 2 O 2 , protect lens epithelial cells or cultured lenses from not only H 2 O 2 stress Yang et al, 1998) but photo-oxidation where superoxide and hydroxyl radicals as well as H 2 O 2 were generated (Spector et al, 1993(Spector et al, , 1997.…”

The Effect of Photochemical Stress Upon the Lenses of Normal and Glutathione Peroxidase-1 Knockout Mice

“…Since GSSG Red is needed to maintain GSH, it is not possible to determine how much of the GSSG Red dependency is due to the GSHPx requirement for high concentrations of GSH or to non-enzymatic GSH detoxification or possibly other GSH dependent enzymes. Utilizing both murine and rabbit immortal lens epithelial cell lines, Yang et al (1998) have shown that when such cells are transfected with a catalase vector leading to elevated catalase activity, the cells effectively resist oxidative 0014-4835\98\100457j15 $30.00\0…”