The effect of caspase-9 in the differentiation of SH-SY5Y cells

Madadi, Zahra; Akbari‐Birgani, Shiva; Mohammadi, Saeed; Khademy, Mitra; Mousavi, Seyed Asadollah

doi:10.1016/j.ejphar.2021.174138

Cited by 4 publications

(2 citation statements)

References 43 publications

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“…The reduced AKT1phosphorylation plays a role in differentiated cell survival by the reduction of Bad phosphorylation, an Akt-downstream survival mediator. In addition, PMA treatment of U937 A study in 2021 demonstrated an increase of Caspase 9 activity during differentiation (Madadi, Akbari-Birgani, Mohammadi, Khademy, & Mousavi, 2021). Inhibition of AKT1 activity by Ly294002 or Wortmannin significantly decreases Bad and Caspase 9 phosphorylation, promoting apoptosis.…”

Section: Akt1 Players and Cell Differentiationmentioning

confidence: 99%

Akt1 players promote PMA U937 cell line differentiation into macrophage-like cells

Bakheit,

Taurin,

Elamin

et al. 2023

AGJSR

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PurposeMonocytes are a leukocytes’ subset that plays an important role in immunity. Protein kinase B (AKT) is involved in monocytes' survival, proliferation and differentiation. Using phorbol 12-myristate 13-acetate (PMA) as an inducer for cell line U937 differentiation into macrophage-like cells may be used as a model for cancer cell therapy or other biomedical research studies. The authors investigated the Akt1 signaling pathway's involvement with PMA as a differentiating agent and survival in the U937 cell line.Design/methodology/approachPMA was utilized to stimulate the differentiation of the U937 cell line into macrophage-like cells at a concentration of 10 nM. Akt1-phosphorylated Serine 473, Bad-phosphorylated Serine 136 and Caspase9-phosphorylated Serine 196 were tested by flow cytometry for the involvement of the Akt1 signaling pathway during differentiation in addition to the expression of CD14, CD206 and CD83. DNA cell cycle variation analysis was done using PI staining and cell viability and apoptosis detection using Annexin V and PI flow cytometry.FindingsThere was a decrease in phosphorylated Akt1 and Bad activation and an increase in Caspase9 activation, with an increase in surface markers CD14, CD206 and CD83 acquired by PMA-differentiated cells. DNA cell cycle analysis revealed cell accumulation in the G2/M phase and fewer cells in the S phase of PMA-induced U937. Apoptosis induction for Ly294002 or Wortmannin-inhibited cells and part of PMA-induced cells were detected.Originality/valueThese results may be used to create a model for biomedical research studies and advance the understanding of the mechanism involving differentiation of the U937 cell line.

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Section: Akt1 Players and Cell Differentiationmentioning

confidence: 99%

Akt1 players promote PMA U937 cell line differentiation into macrophage-like cells

Bakheit,

Taurin,

Elamin

et al. 2023

AGJSR

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“…[4] In line with that, caspase-9 activity has been observed in the differentiation of myoblasts, neuroblastoma, and leukemic cells. [5][6][7] Furthermore, non-lethal activation of caspase-3 contributed to terminal differentiation of human erythroid cells, [8] differentiation of human or mouse cardiomyocytes, [9][10][11] mouse myoblast, [5] embryonic stem cells (ESCs), [12] as well as embryonic synaptogenesis in zebrafish [13] and chick auditory brainstem development. [14] Neural development in different organisms is also implicated by the caspase-6 function independent of apoptosis.…”

Section: Introductionmentioning

confidence: 99%

A Non‐Apoptotic Pattern of Caspase‐9/Caspase‐3 Activation During Differentiation of Human Embryonic Stem Cells into Cardiomyocytes

Ghorbani,

Shiri,

Alian

et al. 2024

Advanced Biology

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In vitro studies have demonstrated that the differentiation of embryonic stem cells (ESCs) into cardiomyocytes requires activation of caspases through the mitochondrial pathway. These studies have relied on synthetic substrates for activity measurements, which can be misleading due to potential none‐specific hydrolysis of these substrates by proteases other than caspases. Hence, caspase‐9 and caspase‐3 activation are investigated during the differentiation of human ESCs (hESCs) by directly assessing caspase‐9 and ‐3 cleavage. Western blot reveals the presence of the cleaved caspase‐9 prior to and during the differentiation of human ESCs (hESCs) into cardiomyocytes at early stages, which diminishes as the differentiation progresses, without cleavage and activation of endogenous procaspase‐3. Activation of exogenous procaspase‐3 by endogenous caspase‐9 and subsequent cleavage of chromogenic caspase‐3 substrate i.e. DEVD‐pNA during the course of differentiation confirmes that endogenous caspase‐9 has the potency to recognize and activate procaspase‐3, but for reasons that are unknown to us fails to do so. These observations suggest the existence of distinct mechanisms of caspase regulation in differentiation as compared to apoptosis. Bioinformatics analysis suggests the presence of caspase‐9 regulators, which may influence proteolytic function under specific conditions.

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Differentiating SH-SY5Y Cells into Polarized Human Neurons for Studying Endogenous and Exogenous Tau Trafficking: Four Protocols to Obtain Neurons with Noradrenergic, Dopaminergic, and Cholinergic Properties

Langerscheidt,

Bell-Simons,

Zempel

2024

Methods in Molecular Biology

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The effect of caspase-9 in the differentiation of SH-SY5Y cells

Cited by 4 publications

References 43 publications

Akt1 players promote PMA U937 cell line differentiation into macrophage-like cells

Akt1 players promote PMA U937 cell line differentiation into macrophage-like cells

A Non‐Apoptotic Pattern of Caspase‐9/Caspase‐3 Activation During Differentiation of Human Embryonic Stem Cells into Cardiomyocytes

Differentiating SH-SY5Y Cells into Polarized Human Neurons for Studying Endogenous and Exogenous Tau Trafficking: Four Protocols to Obtain Neurons with Noradrenergic, Dopaminergic, and Cholinergic Properties

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