The study investigated the impact of career trajectory on employee retention in public university teaching hospitals in South-South Nigeria. A cross-sectional survey research design method was adopted. Out of 357 questionnaires administered to nurses and doctors, a total of 313 questionnaires were retrieved representing an 87.7% return rate. The data collected were subsequently subjected to both descriptive and multiple regression analysis. Findings revealed a positive significant relationship between organisational support and employees retention (β=0.281, t = 2.651, P =0.008<0.05) and equally, a positive significant relationship between organisational commitment and employees’ retention (β =0.678, t =6.385, P= 0.000< 0.05). This study suggested among others that hospital management should promote career development, staff welfare, and staff wellness in order to motivate personnel to stay with the hospitals.