The effect of carbon source succession on laccase activity in the co-culture process of Ganoderma lucidum and a yeast

Li, Ping; Wang, Hailei; Liu, Guosheng; Li, Xin; Yang, Jianming

doi:10.1016/j.enzmictec.2010.07.005

Cited by 45 publications

(28 citation statements)

References 28 publications

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“…Kachlishvili et al [38], among seven carbon sources studied found, after glucose, glycerol as good carbon source to increasing the Lcc activity in Cerrena unicolor . In a co-culture process, Li et al [39] reported to Ganoderma lucidum that, under the condition of glucose deprivation, the use of glycerol as a secondary carbon source produced by the yeast Candida sp. HSD07An leads to the overproduction of Lcc.…”

Section: Resultsmentioning

confidence: 99%

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

Ottoni

Simões

Fernandes

et al. 2016

IJERPH

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Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L−1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.

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Section: Resultsmentioning

confidence: 99%

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

Ottoni

Simões

Fernandes

et al. 2016

IJERPH

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“…Li et al found that glycerol produced from glucose by the yeast Candida sp. is an efficient carbon source for G. lucidum upon glucose deprivation and crucial for laccase overproduction by prolonging laccase secretion time (Li et al, 2011). Phenolics and lysing enzymes produced by opposing microbes have also been suggested to have laccase-inducing ability (Zhang et al, 2006; Wei et al, 2010).…”

Section: Regulation Of Laccase Expressionmentioning

confidence: 99%

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

et al. 2017

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Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas. There have been over two dozen reviews on laccases since 2010 covering various aspects of this group of versatile enzymes, from their occurrence, biochemical properties, and expression to immobilization and applications. This review is not intended to be all-encompassing; instead, we highlighted some of the latest developments in basic and applied laccase research with an emphasis on laccase-mediated bioremediation of pharmaceuticals, especially antibiotics. Pharmaceuticals are a broad class of emerging organic contaminants that are recalcitrant and prevalent. The recent surge in the relevant literature justifies a short review on the topic. Since low laccase yields in natural and genetically modified hosts constitute a bottleneck to industrial-scale applications, we also accentuated a genus of laccase-producing white-rot fungi, Cerrena, and included a discussion with regards to regulation of laccase expression.

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“…Pelleted mycelia (5 ml, $1.0 mm in diameter) were transferred into a 250 mL flask containing 50 mL of liquid medium. The liquid medium for laccase production was modified from Birhanli's method [18] 4 Cl, 0.27 g; CuSO 4 Á5H 2 O, 0.02 g. The pH of the culture broth for laccase production was adjusted with 1 mol/L HCl or NaOH to 4.0 during the fermentation process. All the culture in flasks was carried out on a rotary shaker at 150 r/min, 26°C.…”

Section: Culture Media and Conditionsmentioning

confidence: 99%

“…Laccases can oxidize a wide range of compounds, such as phenolic compounds, aromatic amines, and nonphenolic substrates in the presence of redox mediators [2]. Due to their broad substrate spectrum, laccases are extensively used for various purposes such as pulp delignification and bleaching, dye decolorization, wastewater treatment, food processing, biopolymer modification, biosensors, and bioremediation of environmental pollutants [3,4]. The high demand for laccase, coupled with a long fermentation period and low laccase yield in its production, can limit its use in industrial applications, and highlights the importance of improving laccase production [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Due to their broad substrate spectrum, laccases are extensively used for various purposes such as pulp delignification and bleaching, dye decolorization, wastewater treatment, food processing, biopolymer modification, biosensors, and bioremediation of environmental pollutants [3,4]. The high demand for laccase, coupled with a long fermentation period and low laccase yield in its production, can limit its use in industrial applications, and highlights the importance of improving laccase production [4,5]. A few strategies have been reported to improve the production of laccase, including the isolation and breeding of high producing strains, optimization of the medium, heterologous expression of laccase genes, and the utilization of inducers [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-intensified laccase production from Trametes versicolor

Wang

Chen

et al. 2013

Ultrasonics Sonochemistry

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a b s t r a c tAn efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture.

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The effect of carbon source succession on laccase activity in the co-culture process of Ganoderma lucidum and a yeast

Cited by 45 publications

References 28 publications

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Ultrasound-intensified laccase production from Trametes versicolor

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