The effect of carbon source on plant regeneration in tomato

Gubiš, Jozef; Lajchová, Z.; Klčová, Lenka

doi:10.17221/3759-hortsci

Cited by 8 publications

(5 citation statements)

References 9 publications

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“…with 69% shooting frequency. Previous studies have shown that high cytokinins favor short-time shoot multiplication in tomato in 13 different tomato cultivars [25,26]. In our study, the whole organogenesis process completed in less than 2.5 months for Riogrande with 3 weeks of callogenesis, 3 weeks for shoot multiplication, and 12 days of rooting.…”

Section: Discussionsupporting

confidence: 49%

Efficient and reproducible somatic embryogenesis and micro propagation in tomato via novel structures -Rhizoid Tubers

Saeed

Naseem

Gohar

et al. 2018

Preprint

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10An improved and highly reproducible system for invitro regeneration via somatic 11 embryogenesis (S.E), applicable to several varieties of tomato (cv. Riogrande, cv. Roma 12 grande, hybrid 17905 and model cv. M82) has been developed. First, we developed a 13 conventional indirect organogenesis for all four varieties used in this study. The 14 cotyledons and hypocotyls of 6-day-old tomato were used as explants (1-2 cm) for callus 15 induction (CI) on different callus induction media (CIM) T 0 -T 12 (6-Benzylaminopurine 16 BAP, NAA Naphthalene acetic acid, ZEA Zeatin, IAA Indole-3-acetic acid, KIN 17 Kinetin). Maximum CI response was seen on CIMT 6 (0.5 mg/L NAA, 1 mg/L BAP) and 18 CIMT 7 (2 mg/L IAA, 2 mg/L NAA, 2 mg/L BAP, 4mg/L KIN) in a period of 2 weeks 19 for commercial varieties cvs. Riogrande and Roma. However, cv. M82 responded after 4 20 weeks to a combination of treatments [CIMT 6 (0.5 mg/L NAA + 1 mg/L BAP) and 21 CIMT 8 (2 mg/L IAA + 2 mg/L NAA + 2 mg/L BAP + 4 mg/L ZEA)] for the production 22 of calli. The Riogrande, being the most responsive commercial variety, was selected for 23 invitro morphogenesis via S.E. During S.E. young cotyledons and hypocotyls explants 24 were tested on media with different ranges of pH (3 -7) supplemented with 0.5 and 2 25 mg/L NAA. Resultantly, numerous rhizoids (~38) were produced from each explant at 2 26 pH4 in dark conditions. Further incubation of each rhizoid under light conditions led to 27 the formation of a novel structure -rhizoid tubers (RTBs) on MS media supplemented 28 with 5 mg/L TDZ/BAP at pH4. We observed that only lower pH-induced rhizoids and 29 RTBs regenerated into multiple individual shoots on media at normal pH (5.8). The RTBs 30 led to a complete plantlets regeneration in 45 days compared to the conventional invitro 31 morphogenesis (60 days).32

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Section: Discussionsupporting

confidence: 49%

Efficient and reproducible somatic embryogenesis and micro propagation in tomato via novel structures -Rhizoid Tubers

Saeed

Naseem

Gohar

et al. 2018

Preprint

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“…In tomato, the effect of sugars was significantly different with maltose giving the highest number of shoots followed by glucose (El-Bakry, 2002). Whereas, in other study, Gubiš et al (2005) found that among sugar types sucrose at 3% induced the highest number of shoots in tomato.…”

Section: Introductionmentioning

confidence: 68%

Response of the Strawberry Cv. Elsanta Micro Propagation in Vitro toDifferent Carbon Sources and Concentrations

Abdullah

Al-Khateeb

Layous

2013

JJAS

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The study was conducted to determine the influences of carbon sources (sucrose, glucose, fructose, mannitol and sorbitol) on multiplication and rooting medium of strawberry Elsanta cv. The sugar cources were compared at a relatively wide range of concentrations (10, 30, 50, 70, and 90g l -1 ). There was a clear effect of sugar type and concentrations on the shoot multiplication rate. For each sugar added, the highest number of shoots was formed at concentration of 30g l -1 , in particular, the glucose which gave the heighest nomber of shoots. The sugars concentration lower or higher than 30 gl -1 reduce the number of shoots produced especially with concentrations of 70 and 90 g l -1 , respectively. Along with increasing sugar concentration, the height and fresh weight of shoots is reduced. Sucrose and glucose resulted in the greatest rate of proliferation, while the total number of shoots was lowest when the medium was supplemented with mannitol or sorbitol. On a rooting medium supplied with 10-90 g l -1 of mannitol or sorbitol, the explants did not grow, and subsequently they died after 2-3 weeks. In addition, there were no significant differences between glucose, fructose, and sucrose in the rooting percent, number of roots, number of leaves, and plantlet height, except in root length which showed some significant differences between these carbon sources. In contrast, there were significant differences between the concentrations of these carbon sources. The best rooting response of strawberry occurred on concentrations of 30, 50, and 70 g l -1 , respectively. At acclimatization stage, the plants cultured in fructose supplemented medium produced the highest number of leaves, fresh weight, and dry weight. Moreover, there were significant differences at the final harvest between the concentrations of sugar used. The vegetative growth of the plants was enhanced by increasing the level of sugars especially the concentration 70g l -1 which was the superior to (10, 30 gl -1 ). University, England. Anwar, H. Md.; Taslim H.Md.; Raihanali Md. and Mahbubur Rahman S. M. 2005. Effect of different carbon sources on in vitro regeneration of Indian Penny wort (Centella asiatica L.). Pak. J. Biol. Sci. 8, (7)963 -965. Bogunia, H. and Przywara, L. 1999. Role of carbohydrates in plant tissue culture. Wiadomosci Botaniczne, 43: 25-36.

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“…Therefore, an exogenous source of carbohydrate in the culture medium supplies the required energy for biosynthetic metabolic reactions and as osmotic agents (Eckstein et al, 2012;Pahnekolayi et al, 2019;Phillips and Garda, 2019;Yaseen et al, 2012;Zhang et al, 2017). Several carbohydrates have been used as an exogenous source of energy in in vitro condition, including sucrose (Naik et al, 2010;Siwach et al, 2011), sucrose-derived hexoses (glucose and fructose) (Bahmani et al, 2009;Cheong and An, 2015;Eckstein et al, 2012;Gubis et al, 2005;Kalinina and Brown, 2007), and polyols (sorbitol and mannitol) (Nacheva and Gercheva, 2009;Pathak and Dhawan, 2012;. Indeed, the results were different according to the source, concentration, species and even within a species.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cytokinins, Carbohydrate Source and Auxins on In Vitro Propagation of the ‘G × N-9’ Peach Rootstock

Ritterbusch

Lucho

Radmann

et al. 2020

International Journal of Fruit Science

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The cultivar 'G × N-9' is an almond x peach hybrid rootstock, the main characteristic of which is resistance to several root-knot nematodes (Meloidogyne spp.). Although its propagation by hardwood cutting in the fall is recommended, optimization of 'G × N-9' in vitro propagation is currently needed. Thus, the objective of this study was to evaluate the effect of the cytokinins [6-benzylaminopurine (BAP) and Kinetin (KIN)], and the carbohydrate sources (sucrose and sorbitol) on shoot proliferation and elongation, as well as the influence of indole-3-butyric acid (IBA) on in vitro rooting and acclimatization of 'G × N-9' rootstock. This study reports enhanced on shoot proliferation and elongation based on the interaction between BAP and KIN. When comparing the effects of sorbitol and sucrose, sorbitol at 15 and 30 mg L −1 was the most effective for the induction of shoot proliferation (5.75 ± 0.19 and 6.39 ± 0.21 shoots per explant, respectively). The highest number of roots per explant was in QL medium supplemented with 0.6 and 1.2 mg L −1 IBA. After 30 days of acclimatization, the treated-plants with 1.2 mg L −1 IBA showed the smallest root length, but the higher number of roots per explant (8.35 ± 0.41). The successful acclimatization of plantlets depended on the use of IBA during in vitro culture, to induce a healthy and well-developed root system.

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The effect of carbon source on plant regeneration in tomato

Cited by 8 publications

References 9 publications

Efficient and reproducible somatic embryogenesis and micro propagation in tomato via novel structures -Rhizoid Tubers

Efficient and reproducible somatic embryogenesis and micro propagation in tomato via novel structures -Rhizoid Tubers

Response of the Strawberry Cv. Elsanta Micro Propagation in Vitro toDifferent Carbon Sources and Concentrations

Effect of Cytokinins, Carbohydrate Source and Auxins on In Vitro Propagation of the ‘G × N-9’ Peach Rootstock

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