The effect of carbon dioxide rich environment on carbonic anhydrase activity, growth and metabolite production in indigenous freshwater microalgae

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

“…The only paper found in the literature about the production of CA by cyanobacteria‐ Spirulina sp. and Synechococcus nidulans , was published by the present research group; however, several studies evaluated the production profile of CA by other microalgae, specifically Corallina officinalis , Scenedesmus obliquus , Dunaliella salina , Tetraselmis gracilis , Desmodesmus sp., Kirchneriella sp., and Acutodesmus sp . Several authors have reported that CA activity is induced by low concentrations of dissolved CO 2 .…”

“…Saleh et al studied the production of various pigments (C‐PC, APC, C‐phycoerythrin, carotenoids, and β‐carotene) from different strains of Spirulina , and Chen et al analyzed C‐PC production during CO 2 bio‐fixation by the cyanobacteria Spirulina platensis . There are also some studies about CA production during microalgae cultivation: Basu et al, Li et al, Rigobello‐Masini et al, Swarnalatha et al, and more recently, the present research group studied the co‐production of phycobiliproteins and carbonic anhydrase from Spirulina sp. LEB 18 and Synechococcus nidulans , for which both cyanobacteria were cultivated in Zarrouk's medium diluted to 20 % (v/v) with distilled water .…”

Enhancement of nutrient removal from swine wastewater digestate coupled to biogas purification by microalgae Scenedesmus spp.

“…Not surprisingly, microalgae culturing media augmented with different ratios of CO 2 , is shown to increment biomass productivity (for review see Table 1). CO 2 -rich gases can be obtained at low cost (e.g., flue gases) depending on the overall transportation logistics and distance from the source (Swarnalatha et al, 2015;Zhou et al, 2014). In agricultural areas, biodigesters are extensively used for reducing organic matter load from swine wastewater effluents through transformation of carbon into biogas as a sustainable and renewable source of fuel (Fuchsz and Kohlheb, 2015).…”

Biomass Production of Three Biofuel Energy Plants’ Use of a New Carbon Resource by Carbonic Anhydrase in Simulated Karst Soils: Mechanism and Capacity

“…Using a stable carbon isotope technique, we deduced that the CA in microalgae catalyzed the dissolution of bicarbonate from limestone for biomass production, with a three-to seven-fold increase in biomass production compared to those with an inhibited CA activity treatment. In the study of Swarnalatha et al [20], biomass productivity and carbon dioxide bio-fixation increased two-to four-fold in treated cultures of indigenous freshwater microalgae compared to controlled cultures after 16 days. They attributed these results to the high CA activity in treatment cultures, which increased with the increase in pH and bicarbonate concentration, due to the high concentration of CO 2 in a closed photo-bioreactor.…”

“…Using a method based on stable carbon isotopes, we quantified the proportion of bicarbonate use by plants [11]. Although the conversion to biomass from bicarbonate by the activity of CA has been proposed in studies of plant biomass production for energy [17,18,20], the mechanism and the capacity for bicarbonate use in plants in karst habitats are not fully understood. In this study, we hypothesized that CA activity of plants increased under drought stress, which produced H 2 O and CO 2 from the bicarbonate (HCO 3 − ) in limestone dissolution of karst soils for use in photosynthesis and biomass production.…”