The effect of carbon and nitrogen sources on bovicin HC5 production byStreptococcus bovisHC5

Abstract

Aims: To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. Methods and Results: Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell‐free and cell‐associated bovicin HC5 was determined in culture supernatants and acidic extracts obtained from cell pellets, respectively. Streptococcus bovis HC5 produced bovicin using a variety of carbon and nitrogen sources. The highest specific activity was obtained in media containing 16 g l−1 of glucose, after 16 h of incubation. The peak in cell‐free and cell‐associated bovicin HC5 activity was detected when S. bovis HC5 cultures reached stationary phase. The bovicin HC5 specific activity and bacterial cell mass increased approximately 3‐fold when yeast extract and trypticase (0·5 and 1·0 g l−1, respectively) were added together to the basal medium. Streptococcus bovis HC5 cultures produced bovicin HC5 in cheese whey and sugar cane juice and maximal volumetric productivity was obtained after 12 h of incubation. Conclusions: Streptococcus bovis HC5 is a versatile lactic acid bacterium that can utilize several carbon and nitrogen sources for bovicin HC5 production. This bacterium could be a useful model to study bacteriocin production in the rumen ecosystem. Significance and Impact of the Study: The use of agroindustrial residues as carbon sources could have an economical impact on bovicin HC5 production. To our knowledge, this is the first report to show the use of sugar cane juice for bacteriocin production by lactic acid bacteria.

Rapid Evaluation and Optimization of Medium Components Governing Tryptophan Production by Pediococcus acidilactici TP-6 Isolated from Malaysian Food via Statistical Approaches

“…Furthermore, P. acidilactici TP-6 was able to utilize an array of carbon sources for its growth. This is in agreement with the findings reported by several studies, whereby various LAB were demonstrated to have the capability to utilize various carbon sources for their growth [55][56][57].…”

Effects of carbon and nitrogen sources on bacteriocin-inhibitory activity of postbiotic metabolites produced by Lactobacillus plantarum I-UL4

“…The results from the effects of carbon sources on bacteriocin-inhibitory activity of the postbiotic metabolites produced by L. plantarum I-UL4 are also in agreement with findings from other studies, where glucose was reported to be the preferential carbon source for bacteriocin production by Streptococcus bovicin (Carvalho et al, 2008) and Lactobacillus sakei (Todorov et al, 2012). However, other carbon sources which support optimal bacteriocin production have also been reported for other LAB strains (Cheigh et al, 2002;Drosinos et al, 2005, Wang et al, 2010.…”

“…Indeed, the effect of yeast extract as a single organic nitrogen source on the improvement of bacteriocin activity has been reported for Streptococcus bovis (Carvalho et al, 2008), Lactobacillus rhomanus (Sarika et al, 2010) and Lactobacillus sakei (Todorov et al, 2012). It is likely that the free amino acids and growth factors present in the yeast extract (Aasen et al, 2000) contributed to the stimulatory effect on the production of bacteriocin-inhibitory compounds by L. plantarum I-UL4.…”

Optimization for the maximum bacteriocin production of Lactobacillus brevis DF01 using response surface methodology

“…Because of the growth associated bacteriocin production and the limited number of catabolic pathways, which provide energy to the LAB (substrate level phosphorylation), cell synthesis and the energy demanding bacteriocin production is limited. Several LABs seem to use glucose preferentially for bacteriocin production, and previous studies indicated that Lactococcus lactis, Streptococcus pyogenes, and Streptococcus bovis also preferred glucose for nisin Z, streptococcin SA-FF22, and bovicin HC5 production, respectively (25)(26)(27). Similarly Leuconostoc mesenteroides and Lactobacillus curvatus preferred glucose as carbon source for bacteriocin production (28).…”