The effect of carabrone on mitochondrial respiratory chain complexes in Gaeumannomyces graminis

Ren

Molecular Plant Pathology

et al. 2020

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 67%

“…We further reported that the mitochondrial respiratory chain complex III in G. tritici is highly sensitive to carabrone and is a potential target of carabrone (Wang et al, 2017c).…”

Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 94%

See 2 more Smart Citations

A functional analysis of mitochondrial respiratory chain cytochrome bc₁ complex in Gaeumannomyces tritici by RNA silencing as a possible target of carabrone

Ren

Molecular Plant Pathology

et al. 2020

“…The fungus was grown on potato dextrose agar (PDA) at 25 °C for 5–7 days before being used. The strain was stored at −80 °C by cryopreservation in 30% glycerol solution [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Optimization of Production Conditions for Protoplasts and Polyethylene Glycol-Mediated Transformation of Gaeumannomyces tritici

et al. 2018

Take-all, caused by Gaeumannomyces tritici, is one of the most important wheat root diseases worldwide, as it results in serious yield losses. In this study, G. tritici was transformed to express the hygromycin B phosphotransferase using a combined protoplast and polyethylene glycol (PEG)-mediated transformation technique. Based on a series of single-factor experimental results, three major factors—temperature, enzyme lysis time, and concentration of the lysing enzyme—were selected as the independent variables, which were optimized using the response surface methodology. A higher protoplast yield of 9.83 × 107 protoplasts/mL was observed, and the protoplast vitality was also high, reaching 96.27% after optimization. Protoplasts were isolated under the optimal conditions, with the highest transformation frequency (46–54 transformants/μg DNA). Polymerase chain reaction and Southern blotting detection indicated that the genes of hygromycin phosphotransferase were successfully inserted into the genome of G. tritici. An optimised PEG-mediated protoplast transformation system for G. tritici was established. The techniques and procedures described will lay the foundation for establishing a good mutation library of G. tritici and could be used to transform other fungi.

Pest Management Science

Mining and characterization of oxidative stress‐related binding proteins of parthenolide in Xanthomonas oryzae pv. oryzae

Zhou

Liu

et al. 2022

BACKGROUND: Lack of control agents and development of bacterial resistance are emergent problems in the chemical control of rice bacterial blight, therefore novel bactericides against Xanthomonas oryzae pv. oryzae (Xoo, the causal agent of rice bacterial blight) are urgently needed. We previously found that parthenolide (PTL) is a potential lead against Xoo, and PTL inhibits Xoo growth via oxidative stress. However, the mechanism of action of PTL against Xoo needs further elucidation.RESULTS: In this study, a biotinylated PTL probe was synthesized, and two important subunits in the respiratory chain (NuoF of complex I and SdhB of complex II) of Xoo were captured with the probe and identified with liquid chromatography tandem mass spectrometry (LC-MS/MS). The binding between them was verified with pull-down and drug affinity responsive target stability technologies. In addition, purified proteins of NuoF and SdhB greatly lowered the antibacterial activity of PTL, and PTL evidently inhibited the enzyme activities of complexes I and II. Moreover, knockout of nuoF and sdhB in Xoo caused elevated reactive oxygen species (ROS) levels and increased sensitivity to PTL. Furthermore, molecular simulations indicated that PTL may form covalent bonds with Cys105 and Cys187 in NuoF and Cys106 in SdhB.CONCLUSION: PTL can directly bind to NuoF and SdhB, which impairs the enzyme functions of complexes I and II in the respiratory chain, leading to ROS accumulation in Xoo. This study will provide deep insight into the mechanism of action of PTL against Xoo.