The effect of calcium on the growth ofChlorella andScenedesmus

Dvořáková-Hladká, Jiřina

doi:10.1007/bf02922808

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…These include ion transport and as a secondary messenger of various responses to abiotic and biotic stimuli including light, high and low temperature [22]. Previous studies reported that calcium deficiency could cause the number of daughter cells to reduce and inhibition of formation of autospores [23].Thus, it is important to make sure the concentration of calcium is carefully regulated to prevent osmotic and metal stress which can lead to inhibition of algal cells [24]. Magnesium also was preferred at high concentration for the growth of microalgae.…”

Section: A Improvement Of N Oleoabundans Cultivation Mediummentioning

confidence: 99%

Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System

Ibrahim

Radzun

Ismail

2018

International Journal on Advanced Science, Engineering and Information Technology

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Microalgal biomass has been regarded as one of the most promising commodities for biofuels production, fertilisers, animal food and high-value products. The capacity to generate high microalgal biomass is hence crucial to increase the number of the product that can be derived microalgal biomass. Recently, an advanced high-throughput nutrient screen was used to optimise the nutrient formulation to maximise the growth of Neochloris oleoabundans under photoautotrophic condition. The screen was designed to optimise 10 nutrients via the Box-Behnken experimental design that established a screen comprising 180 media formulations matrix for microalgae cultivation (150 µL microplate-based miniature-scale cultivation system). Improved cultivation media were formulated for N. oleoabundans via the nutrient screening system. Medium formulation no. 51 and 76 (from the 180 media formulation) yielded the highest end-point OD 560 of 1.319 ±0. 009 and 1.345 ±0. 023 respectively and both formulations were redesignated as medium M1 and M2 respectively for subsequent experiments. Reproducibility assessment of both media (1 L larger scale experiments) was in concordance with miniature scale data. Nutrient main effect statistical analysis suggested that modulation of Ca, Mg, Fe, Mn and Zn concentrations may further improve the N. oleoabundans growth performance.

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Section: A Improvement Of N Oleoabundans Cultivation Mediummentioning

confidence: 99%

Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System

Ibrahim

Radzun

Ismail

2018

International Journal on Advanced Science, Engineering and Information Technology

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“…Generally, Ca 2+ acts as a secondary messenger in various cellular processes, including cell proliferation, development, motility, secretion, learning, and memory, thereby influencing nutrient assimilation, growth, and stress adaptation in microalgae and plants [ 1 ]. It has been observed that Ca 2+ enhances the uptake of nitrogen, organic carbon sources and phosphorus, leading to improved growth rates in Chlorella pyrenoidosa [ 2 ] and Scenedesmus sp. [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Calcium Depletion on Proliferation of Chlorella sorokiniana Strain DSCG150

Kang,

Cho,

Jeong

et al. 2024

J. Microbiol. Biotechnol.

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This study analyzed the effects of Ca 2+ metal ions among culture medium components on the Chlorella sorokiniana strain DSCG150 strain cell growth. The C. sorokiniana strain DSCG150 grew based on a multiple fission cell cycle and growth became stagnant in the absence of metal ions in the medium, particularly Ca 2+ . Flow cytometry and confocal microscopic image analysis results showed that in the absence of Ca 2+ , cell growth became stagnant as the cells accumulated into four autospores and could not transform into daughter cells. Genetic analysis showed that the absence of Ca 2+ caused upregulation of calmodulin ( calA ) and cell division control protein 2 ( CDC2_1 ) genes, and downregulation of origin of replication complex subunit 6 ( ORC6 ) and dual specificity protein phosphatase CDC14A ( CDC14A ) genes. Analysis of gene expression patterns by qRT-PCR showed that the absence of Ca 2+ did not affect cell cycle progression up to 4n autospore, but it inhibited Chlorella cell fission (liberation of autospores). The addition of Ca 2+ to cells cultivated in the absence of Ca 2+ resulted in an increase in n cell population, leading to the resumption of C. sorokiniana growth. These findings suggest that Ca 2+ plays a crucial role in the fission process in Chlorella .

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The effect of calcium on the growth ofChlorella andScenedesmus

Cited by 5 publications

References 16 publications

Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System

Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System

The Impact of Calcium Depletion on Proliferation of Chlorella sorokiniana Strain DSCG150

Media optimization design towards maximizing biomass production of Tetradesmus obliquus under mixotrophic conditions

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