The effect of calcium chloride concentration on alginate/Fmoc-diphenylalanine hydrogel networks

Çelik, Ekin; Bayram, Cem; Akçapınar, Rümeysa; Türk, Mustafa; Denkbaş, Emir Baki

doi:10.1016/j.msec.2016.04.084

Cited by 51 publications

(36 citation statements)

References 50 publications

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“…The cell delivery vehicle needs to disperse cells evenly in a three-dimensional scaffold, to protect and maintain cell viability and functionality, and to deliver cells in a timely and controlled manner. Hydrogels, based on both natural and synthetic polymers, have been used for this purpose [17,36]. Cells are typically encapsulated by gelling cell suspension in a hydrogel solution to form a solidified matrix.…”

Section: Discussionmentioning

confidence: 99%

“…An ideal cell delivery vehicle should be able to protect cells from hazards during CPC setting and the injection operation, and is fast degradable to release the viable cells throughout the scaffold after CPC has set. To fabricate such a cell delivery system, natural hydrogel biopolymers, such as alginate, are of great interest, because they can be cross-linked under mild conditions to form highly hydrated gel with good biocompatibility and better mechanical properties [16][17][18]. One major concern is that alginate degrades very slowly and uncontrollably in mammals [19].…”

Section: Introductionmentioning

confidence: 99%

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Engineering bone regeneration with novel cell-laden hydrogel microfiber-injectable calcium phosphate scaffold

Song

Zhang

Wang

et al. 2017

Materials Science and Engineering: C

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Cell-based tissue engineering is promising to create living functional tissues for bone regeneration. The implanted cells should be evenly distributed in the scaffold, be fast-released to the defect and maintain high viability in order to actively participate in the regenerative process. Herein, we report an injectable calcium phosphate cement (CPC) scaffold containing cell-encapsulating hydrogel microfibers with desirable degradability that could deliver cells in a timely manner and maintain cell viability. Microfibers were synthesized using partially-oxidized alginate with various concentrations (0-0.8%) of fibrinogen to optimize the degradation rate of the alginate-fibrin microfibers (Alg-Fb MF). A fibrin concentration of 0.4% in Alg-Fb MF resulted in the greatest enhancement of cell migration, release and proliferation. Interestingly, a significant amount of cell-cell contact along the long-axis of the microfibers was established in Alg-0.4%Fb MF as early as day 2. The injectable tissue engineered construct for bone reconstruct was fabricated by mixing the fast-degradable Alg-0.4%Fb MF with CPC paste at 1:1 volume ratio. In vitro study showed that cells re-collected from the construct maintained good viability and osteogenic potentials. In vivo study demonstrated that the hBMSC-encapsulated CPC-MF tissue engineered construct displayed a robust capacity for bone regeneration. At 12weeks after implantation, osseous bridge in the rat mandibular defect was observed in CPC-MF-hBMSCs group with a new bone area fraction of (42.1±7.8) % in the defects, which was >3-fold that of the control group. The novel tissue-engineered construct presents an excellent prospect for a wide range of dental, craniofacial and orthopedic applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering bone regeneration with novel cell-laden hydrogel microfiber-injectable calcium phosphate scaffold

Song

Zhang

Wang

et al. 2017

Materials Science and Engineering: C

View full text Add to dashboard Cite

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“…Alginate particles have been employed as carriers for controlled drug release [17,18]. Sodium alginate contains pendant carboxylate that introduced as a polyanionic copolymer, Physical cross-linking of sodium alginate would be done through electrostatic interactions between aligned glucuronic blocks of alginate chains and polyvalent cations (like Ca 2+ ) [19,20]. Organic and inorganic nanoparticles [21] including metal nanoparticles [22], clay [23], silica [24], magnetic Fe 3 O 4 [25], carbon nanotubes [26] and hydroxyapatite [27] for the synthesis of alginate composite beads with exclusive structure and properties have been used.…”

Section: Introductionmentioning

confidence: 99%

Caffeine: A novel green precursor for synthesis of magnetic CoFe2O4 nanoparticles and pH-sensitive magnetic alginate beads for drug delivery

Amiri

Salavati‐Niasari

Pardakhty

et al. 2017

Materials Science and Engineering: C

184

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Hydrogel beads are promising delivery systems for encapsulation and release of drugs due to the mild process of their fabrication from biopolymers. Magnetic CoFeO nanoparticles (MCFO, 9.72nm in diameter) were synthesized via a co-precipitation method using caffeine as a new environmentally friendly material in order to alkalinize the medium. Drug-targeting Magnetic beads based on CoFeO nanoparticles, sodium alginate and chlorpheniramine maleate (CPAM) were synthesized in the presence of Ca ions to obtain ionic cross-linked magnetic hydrogel beads. Nanoparticles as well as produced magnetic beads were thoroughly characterized by FTIR, XRD, SEM, nanosizer and VSM techniques. The swelling ratio of beads indicated pH-dependent property with maximum water absorbing at pH7.4. The in vitro release of beads exhibited significant behavior on the subject of nanoparticles concentration and alginate content. Biocompatibility of the CFO nanoparticles and MCFO/Alg beads are demonstrated through cytotoxicity test via MTT assay on U87 cell lines.

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“…The optical microscope image of the honeycomb-like structure, scale bar=100μm(5). High-speed camera frame of T-junction module, scale bar=500μm(6).A phantom 7.3 high speed camera with a maximum resolution of 800 * 600 pixels at up to 4800 fps giving recording duration of 1.2 seconds (Vision Research Ltd., UK) was utilized to record the formation of the micro droplets inside the T-junction channel and at the tip end with real time video images.2.4. Characterization of 2D and 3D Honeycomb-like Structures 2D and 3D PLGA-b-PEG structures were morphologically examined by Optical Light Microscopy (Zeiss Axiotech, Germany) fitted with a camera (Nikon Eclipse ME 600, Japan) and Scanning Electron Microscopy (SEM, Hitachi S3400, Japan).…”

mentioning

confidence: 99%

Honeycomb-like PLGA-b-PEG Structure Creation with T-Junction Microdroplets

Gultekinoglu¹,

Jiang

Bayram³

et al. 2018

Langmuir

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Amphiphilic block copolymers are widely used in science owing to their versatile properties. In this study, amphiphilic block copolymer poly(lactic- co-glycolic acid)- block-poly(ethylene glycol) (PLGA- b-PEG) was used to create microdroplets in a T-junction microfluidic device with a well-defined geometry. To compare interfacial characteristics of microdroplets, dichloromethane (DCM) and chloroform were used to prepare PLGA- b-PEG solution as an oil phase. In the T-junction device, water and oil phases were manipulated at variable flow rates from 50 to 300 μL/min by increments of 50 μL/min. Fabricated microdroplets were directly collected on a glass slide. After a drying period, porous two-dimensional and three-dimensional structures were obtained as honeycomb-like structure. Pore sizes were increased according to increased water/oil flow rate for both DCM and chloroform solutions. Also, it was shown that increasing polymer concentration decreased the pore size of honeycomb-like structures at a constant water/oil flow rate (50:50 μL/min). Additionally, PLGA- b-PEG nanoparticles were also obtained on the struts of honeycomb-like structures according to the water solubility, volatility, and viscosity properties of oil phases, by the aid of Marangoni flow. The resulting structures have a great potential to be used in biomedical applications, especially in drug delivery-related studies, with nanoparticle forming ability and cellular responses in different surface morphologies.

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The effect of calcium chloride concentration on alginate/Fmoc-diphenylalanine hydrogel networks

Cited by 51 publications

References 50 publications

Engineering bone regeneration with novel cell-laden hydrogel microfiber-injectable calcium phosphate scaffold

Engineering bone regeneration with novel cell-laden hydrogel microfiber-injectable calcium phosphate scaffold

Caffeine: A novel green precursor for synthesis of magnetic CoFe2O4 nanoparticles and pH-sensitive magnetic alginate beads for drug delivery

Honeycomb-like PLGA-b-PEG Structure Creation with T-Junction Microdroplets

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