The effect of caffeine on the ability of spermatozoa to fertilize mature human oocytes

Imoedemhe, D. A. G.; Sigue, Alejandro B.; Pacpaco, Edgardo Luis A.; Olazo, Arturo B.

doi:10.1007/bf01203756

Cited by 37 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Treatment of sperm with caffeine is common in some hooved species, and while treatment improves IVF rates it can also decrease the rate of blastocyst formation (Tatham et al, 2003;Mao et al, 2005). Like wise, treatment of human sperm with caffeine to improve motility for IVF resulted in delayed embryo development compared to IVF with untreated sperm (Imoedemhe et al, 1992). Similarly, pronuclear mouse embryos incubated with low levels of caffeine (0.16 mM) frequently stopped developing after one to three cell cycles (Scott and Smith, 1995).…”

Section: Discussionmentioning

confidence: 99%

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Tollner¹,

VandeVoort²,

Yudin³

et al. 2008

Molecular Reproduction Devel

View full text Add to dashboard Cite

Capacitation of macaque sperm in vitro has been achieved efficiently only with the addition of both cyclic nucleotides and methylxanthines. The use of these exogenous sperm activators clouds an understanding of the normal mechanisms underlying capacitation and may slow early embryo development following in vitro fertilization (IVF). We demonstrate that culture medium which simulates periovulatory oviductal fluid with respect to bicarbonate (HCO(3)(-)) and glucose concentration induces capacitation in a high percentage of macaque sperm as determined by the ability of sperm to undergo both the release of coating protein DEFB126 and the zona pellucida-induced acrosome reaction (AR). Few sperm were able to undergo the AR following 6 hr incubation in medium containing either 35 mM HCO(3)(-) (approximately 7.2 pH) or 90 mM HCO(3)(-) (approximately pH 7.8) with 5 mM glucose. When glucose concentration was lowered to 0.5 mM to match levels reported for women at midcycle, the AR rate increased significantly in sperm incubated in both levels of HCO(3)(-), indicating that glucose interferes with sperm responsiveness to increasing HCO(3)(-) concentration observed in the primate oviduct during ovulation. Even greater synchronization of capacitation could be achieved with nonphysiologic extremes of alkalinity or energy substrate deprivation. In the latter case, sperm achieved high rates of IVF. A shift in pH from 7.2 to 7.8 in a HEPES-buffered medium was sufficient to remove DEFB126 from the surface of most sperm after only 3 hr. The loss of DEFB126 from sperm under periovulaory fluid conditions has implications for the timing of release of sperm from the oviductal reservoir.

show abstract

Section: Discussionmentioning

confidence: 99%

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Tollner¹,

VandeVoort²,

Yudin³

et al. 2008

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…Moreover, spermatozoa pre-incubated with caffeine showed a significant decrease in the percentage of penetrated zona-free hamster oocytes [117]. The only study available [118] that investigated the effect of caffeine in assisted reproduction using 42 oocytes from 21 women showed an increase in sperm motility but a significant decrease in the fertilization rates and embryo development.…”

Section: In Vitro Treatment Of Spermatozoamentioning

confidence: 99%

Sperm preparation for ART

Henkel

Schill

2003

Reprod Biol Endocrinol

415

175

View full text Add to dashboard Cite

The onset of clinical assisted reproduction, a quarter of a century ago, required the isolation of motile spermatozoa. As the indication of assisted reproduction shifted from mere gynaecological indications to andrological indications during the years, this urged andrological research to understand the physiology of male germ cell better and develop more sophisticated techniques to separate functional spermatozoa from those that are immotile, have poor morphology or are not capable to fertilize oocytes. Initially, starting from simple washing of spermatozoa, separation techniques, based on different principles like migration, filtration or density gradient centrifugation evolved. The most simple and cheapest is the conventional swim-up procedure. A more sophisticated and most gentle migration method is migration-sedimentation. However, its yield is relatively small and the technique is therefore normally only limited to ejaculates with a high number of motile spermatozoa. Recently, however, the method was also successfully used to isolate spermatozoa for intracytoplasmic sperm injection (ICSI). Sperm separation methods that yield a higher number of motile spermatozoa are glass wool filtration or density gradient centrifugation with different media. Since Percoll® as a density medium was removed from the market in 1996 for clinical use in the human because of its risk of contamination with endotoxins, other media like IxaPrep®, Nycodenz, SilSelect®, PureSperm® or Isolate® were developed in order to replace Percoll®. Today, an array of different methods is available and the selection depends on the quality of the ejaculates, which also includes production of reactive oxygen species (ROS) by spermatozoa and leukocytes. Ejaculates with ROS production should not be separated by means of conventional swim-up, as this can severely damage the spermatozoa. In order to protect the male germ cells from the influence of ROS and to stimulate their motility to increase the yield, a number of substances can be added to the ejaculate or the separation medium. Caffeine, pentoxifylline and 2-deoxyadenosine are substances that were used to stimulate motility. Recent approaches to stimulate spermatozoa include bicarbonate, metal chelators or platelet-activating factor (PAF). While the use of PAF already resulted in pregnancies in intrauterine insemination, the suitability of the other substances for the clinical use still needs to be tested. Finally, the isolation of functional spermatozoa from highly viscous ejaculates is a special challenge and can be performed enzymatically to liquefy the ejaculate. The older method, by which the ejaculate is forcefully aspirated through a narrow-gauge needle, should be abandoned as it can severely damage spermatozoa, thus resulting in immotile sperm.

show abstract

“…In one humped camels, El‐Bahrawy () reported that addition 4 mM of caffeine improved sperm kinetics and physical semen characteristics post‐thaw including PG, VCL. Imoedemhe, Sigue, Pacpaco, and Olazo () recorded that caffeine supplementation improved VCL VSL, VAP, LIN, and STR patterns and LIN in fresh human sperm. Yeste et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen

El-Hamid

2018

Reprod Domestic Animals

View full text Add to dashboard Cite

Two experiments were carried out to determine the efficiency of supplementation of ram semen extender with caffeine on chilled storage and frozen capacity of spermatozoa. In the first experiment, eighty ejaculates were collected by an artificial vagina from five adult Barki rams, aged 2-3 years and weighted 45.0 ± 2.0 kg throughout the experimental period (January to February 2017). The ejaculates were pooled and diluted (1:10) with tris-citric egg yolk extender and were split into five groups. Group 1 served as control, whereas groups 2-5 were supplemented with 0.1, 0.2, 0.3 and 0.4 mM caffeine. All diluted semen specimens were evaluated for physical characteristics immediately after dilution (T ) and throughout preservation period of 48 hr at 4°C. Simultaneously, oxidative stress and indices such as total antioxidant capacity (TAC), malondialdehyde concentrations (MDA) and alkaline transaminase (AKP) concentrations and value of resazurin reduction test (RRT) were determined. In the second experiment, the raw pooled ejaculates were diluted (1:10) with glycerolated tris-citric egg yolk extender, receiving the previously mentioned caffeine levels. The post-thaw assessment of cryopreserved spermatozoa, in all groups, was conducted by a computer-assisted sperm analysis (CASA) system. The results revealed that adding caffeine to ram semen extender at low (0.1 mM) or medium (0.2 mM) levels had positive impact on both physical characteristics of ram sperm and the enzymatic activities compared to the other semen groups. Caffeine supplementation also enhanced post-thaw sperm dynamics, which implies its potential as an exogenous antioxidant supplement.

show abstract

The effect of caffeine on the ability of spermatozoa to fertilize mature human oocytes

Cited by 37 publications

References 19 publications

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Sperm preparation for ART

Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen

Contact Info

Product

Resources

About