The effect of cadmium exposure and stress on plasma cortisol, metallothionein levels and oxidative status in rainbow trout (Oncorhynchus mykiss) liver

Tort, L.; Kargačin, B.; Torres, Pere; Giralt, Mercedes; Hidalgo, Juan

doi:10.1016/0742-8413(95)02107-8

Cited by 20 publications

(18 citation statements)

References 28 publications

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“…Some investigations showed that heavy metals, such as Cd, Hg and Pb, increased total glutathione levels in striped mullet (Thomas and Wofford 1984) and rainbow trout (Tort et al 1996) presumably through interference with GSH metabolism (Canesi et al 1999). Schlenk and Rice (1998) opined that such Cdmediated induction of cytosolic thiol-rich proteins (GSH and metallothionein) could protect against secondary induced oxidative stress, probably by maintaining a reduced state in the cell.…”

Section: Discussionmentioning

confidence: 98%

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Dabas

Nagpure

Kumar

et al. 2011

Fish Physiol Biochem

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The effects of different concentrations of cadmium chloride on the extent of lipid peroxidation (LPO) and alterations in the antioxidant enzyme activities were studied in liver, kidney and gill tissues of freshwater murrel, Channa punctatus. The fish specimens were exposed to 6.7, 13.4 and 20.1 mg l(-1) sublethal concentrations of cadmium chloride and the oxidative stress was assessed after 24, 48, 72 and 96 h post-exposure. The biomarkers selected for the study were thiobarbituric acid reactive substances for assessing the extent of lipid peroxidation and antioxidant defense system such as reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GP(X)), glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) activities. In general, the cadmium exposure elevated the LPO in subject tissues of treated group and modulated the activities of GPx, GST, SOD, CAT, GR and level of GSH after given exposure as compared to the control. All enzymes activities, except CAT (in kidney and gills), and amount of LPO elevated significantly (P < 0.05) in treated group with respect to control in all tissues, while significant difference was not observed between the exposed concentrations and within exposure duration. The results indicated that increase in LPO level and the fluctuation in antioxidant defense system in fish could be due to cadmium-induced increase in the production of reactive oxygen species (ROS). The potential role of these parameters as biomarkers of heavy metal pollution in aquatic system is discussed.

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Section: Discussionmentioning

confidence: 98%

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Dabas

Nagpure

Kumar

et al. 2011

Fish Physiol Biochem

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“…Bebianno and Machado (1997) have proposed that measurement of metallothioneins concentrations in biomonitors can provide an accurate indication of sublethal environment increases in metal contamination. Moreover, metallothioneins may be useful in predicting metal toxicity in aquatic organisms because cellular toxicity may result after metal binding capacity of metallothioneins is exceeded and associated with the stress response in aquatic organisms (Cattani et al 1996;Tort et al 1996).…”

Section: Discussionmentioning

confidence: 99%

The effect of in situ cadmium contamination on some biomarkers in Cerastoderma glaucum

et al. 2007

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The present study was designed to detect in situ effects of cadmium on marine organisms Cerastoderma glaucum from the gulf of Gabès (Tunisia). Six sampling stations were chosen: one site, relatively far from known local source of pollution, was considered as uncontaminated site and five sites clearly exposed to anthropogenic impact. Metallothionein like protein (MTLP) and sub-cellular metal partitioning were measured in cockles (C. glaucum) gills and digestive gland. Various biomarkers were also measured, including malondialdehyde (MDA) in the digestive gland and acetylcholinesterase activity (AChE) in the remainder. The health status of the cockles was assessed by using the condition index (CI). Significant differences between sites were noted for Cd concentrations, (CI) and also for the three studied biomarkers. Significant higher biomarkers response was measured in cockles from stations located in the northern part of the gulf, which are exposed directly to industrial and urban effluents, whereas the response of most biomarkers was minimal at the reference station. Positive and significant correlations were observed between MTLP and Cd concentrations in the digestive gland and in the gills. However, it must be noted that these correlations were more significant in the digestive gland, suggesting that compared to the gills, the digestive gland of C. glaucum is more suitable for monitoring metal pollution. The subcellular distribution of Cd showed that the soluble fraction was the major compartment for Cd storage, a pattern which is due to the role of MTLP in Cd detoxication. But at the most contaminated site (EH), cadmium in the digestive gland was preferentially accumulated in the insoluble fraction (P1) suggesting that the MTLP capacity in binding metals was not sufficient to avoid the binding of Cd to the insoluble fraction. Furthermore, the MTLP concentrations in the cockles from this site are lower than expected. So in highly polluted sites, MTLP in C. glaucum should not be used as a useful biomarker for metal pollution. The lipid peroxidation as presented by malondialdehyde levels, and MT-like protein concentrations increased in cockles exposed to cadmium contamination. We can, therefore, hypothesize that Cd could induce MTLP synthesis and MDA increase. While AChE had distinct and specific pattern showing that cadmium is not the only factor of the inhibition of cholinesterase activity. There are other polluting inputs engendering this inhibition.

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“…Segundo Tort et al (1996), o fígado demonstra ser o primeiro órgão a estocar o cobre em peixes em conseqüência desse acúmulo, pode apresentar danos fisiológicos e histopatológicos causados por este elemento.…”

Section: Tabelaunclassified

Níveis de cobre em dietas para a tilápia do Nilo, Oreochromis niloticus

Ferrari

Barros

Pezzato

et al. 2004

Acta Sci. Anim. Sci.

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RESUMO. Os efeitos de diferentes níveis de cobre no desempenho produtivo e nos parâmetros fisiológicos da tilápia do Nilo (Oreochromis niloticus L.) foram avaliados por um período de 120 dias. O experimento foi inteiramente casualizado com 5 níveis de suplementação de cobre (0,0; 4,0; 80,0; 160,0 e 320,0mg/kg como sulfato de cobre heptaidratado, 24,05% cobre) e seis repetições por tratamento. Foram utilizados 180 peixes com peso de 2,0  0,05g distribuídos aleatoriamente em 30 aquários de 60L cada, em uma lotação de 6 peixes/aquário. Os aquários foram dotados de sistema de filtragem individual de água e de sistema de aquecimento controlado por termostato (25,0º  1,0C). Confeccionouse 5 rações purificadas, à base de albumina e gelatina, isoprotéicas (32,0% PB) e isoenergéticas (3200 kcal/kg de energia digestível), diferenciando do controle pela adição de sulfato de cobre. As concentrações de oxigênio dissolvido e de pH foram determinadas semanalmente. Determinou-se o ganho médio de peso, a conversão alimentar aparente e a porcentagem de sobrevivência. Como parâmetros hematológicos, avaliou-se o número de eritrócitos, concentração de hemoglobina, porcentagem de hematócrito e o volume globular médio e a concentração de hemoglobina globular média. Determinou-se a concentração de cobre no fígado, ossos, carcaça e filé e avaliou-se a morfologia e coloração dos fígados. Conclui-se que: para a tilápia do Nilo, a ausência e a utilização de superdosagem de cobre na ração não determinam alterações no desempenho produtivo e na hematologia; a concentração de cobre no fígado é responsiva à quantidade suplementada desse mineral na ração; a concentração elevada do cobre na ração determina alterações hepáticas e, o tempo é fator determinante da ação detrimental do cobre para respostas fisiológicas do peixe.Palavras-chave: mineral, hematologia, toxicidade, sulfato de cobre. ABSTRACT. Copper levels in Nile tilapiaOreochromis niloticus diets. The effects of different levels of dietary copper on growth performance and physiological parameters of Nile tilapia (Oreochromis niloticus L.) were analyzed for 120 days. The experimental design was completely randomized, with five treatments (0.0; 4.0; 80.0; 160.0 and 320.0mg cooper/kg diet as cooper sulfate hepahydrate, 24.05% copper) and six replicates. One hundred and eighty fish, with 2.0  0.05g average initial weight, were kept into 30 aquaria (60L) with individual filter and aeration; water temperature was kept at 25.0  1.0ºC. An albumin-gelatin diet was formulated to contain 32.0% crude protein and 3,200 kcal of digestible energy/kg. The oxygen concentrations and pH were measured weekly. At the end of the experiment, the weight gain, feed conversion ratio and survival rate were determined. Erythrocytes number, hemoglobin concentration, hematocrit ratio, mean corpuscular volume and mean corpuscular hemoglobin concentration were analyzed as hematological parameters. Liver, bones, carcass and muscle copper concentrations were also determined and the liver morphology and coloration we...

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The effect of cadmium exposure and stress on plasma cortisol, metallothionein levels and oxidative status in rainbow trout (Oncorhynchus mykiss) liver

Cited by 20 publications

References 28 publications

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

The effect of in situ cadmium contamination on some biomarkers in Cerastoderma glaucum

Níveis de cobre em dietas para a tilápia do Nilo, Oreochromis niloticus

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