The effect of breed and intrauterine crowding on fetal erythropoiesis on day 35 of gestation in swine1

Vallet, J. L.; Klemcke, Harold G.; Christenson, R. K.; Pearson, Paul L.

doi:10.2527/2003.8192352x

Cited by 17 publications

(21 citation statements)

References 13 publications

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“…Deficiencies in folate lead to abnormal erythropoiesis [3] and birth defects [4]. Previous results suggest that the amount of folate available to the developing conceptus influences fetal erythropoiesis [5] and fetal erythropoiesis influences uterine capacity of swine [5][6][7]. In addition, it has been reported that folate supplements increased the survival rate of fetuses 1 Supported by CSREES grant 2001-35203-11241.…”

Section: Introductionmentioning

confidence: 99%

Secreted and Placental Membrane Forms of Folate-Binding Protein Occur Sequentially During Pregnancy in Swine1

Kim

Vallet

2004

Biology of Reproduction

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The objective was to understand how two forms of folate-binding protein interact to accomplish folate transport during pregnancy in swine. Specific folate binding was measured in uterine flushings during the estrous cycle and early pregnancy and in allantoic fluid (secreted form) and placental membranes (membrane form) throughout later pregnancy. In addition, the localization of the secreted form of folate-binding protein (sFBP) in uterine wall sections was assessed. Uterine flushings were collected on Days 10, 13, and 15 of the estrous cycle and pregnancy. Allantoic fluid and placentas were collected on Days 20, 35, 50, 70, 90, and 105 of pregnancy. Uterine-wall sections were collected on all days of the experiment. Folate binding was measured by incubation of aliquots of uterine flushings, allantoic fluid, or placental microsomal membranes with 0.5-4 nM [(3)H]folate. Uterine-wall sections were incubated with purified anti-FBP IgG or normal rabbit serum IgG to localize sFBP. Folate binding did not differ between early pregnancy and the estrous cycle in uterine flushings, was greatest from Day 50 to 70 of pregnancy in allantoic fluid, and was greatest from Day 50 of pregnancy onward in placental microsomal membranes. Staining for sFBP was present in the endometrial glands from Day 10 to 15 in cyclic gilts and from Day 10 to 20 in pregnant gilts. The pattern of folate binding and sFBP staining supports the concept that sFBP transports folate to the developing conceptus until placentation and then the placental form takes over folate transport.

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Section: Introductionmentioning

confidence: 99%

Secreted and Placental Membrane Forms of Folate-Binding Protein Occur Sequentially During Pregnancy in Swine1

Kim

Vallet

2004

Biology of Reproduction

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“…Furthermore, Vallet et al (2003) suggested that fetal growth rate is less sensitive to intrauterine crowding than placental growth rate and that, as in the prolific Meishan female (Ford & Youngs 1993), an increase in placental efficiency may initially protect the developing fetus from a limitation in placental size. However, conclusions based only on a consideration of fetal weight may overlook critical effects on fetal development established early in gestation.…”

Section: Introductionmentioning

confidence: 99%

Number of conceptuses in utero affects porcine fetal muscle development

Town¹,

Putman²,

Turchinsky³

et al. 2004

Reproduction

123

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Unmodified, third parity, control sows (CTR; n 5 30) or sows subjected to unilateral oviduct ligation before breeding (LIG; n 5 30), were slaughtered at either day 30 or day 90 of gestation and used to determine the effects of numbers of conceptuses in utero on prenatal, and particularly muscle fibre, development. Ovulation rate, number of conceptuses in utero, placental and fetal size, and (day 90 sows) fetal organ and semitendinosus muscle development were recorded. Tubal ligation reduced (P < 0.05) the number of viable embryos at day 30 and fetuses at day 90. Placental weight at day 30 and day 90, and fetal weight at day 90, were lower (P < 0.05) in CTR sows. All body organs except the brain were lighter, and the brain:liver weight ratio was higher in CTR fetuses (P < 0.05), indicative of brain sparing and intrauterine growth restriction in fetuses from CTR sows. Muscle weight, muscle cross-sectional area and the total number of secondary fibres were also lower (P < 0.05) in CTR fetuses. The number of primary fibres, the secondary:primary muscle fibre ratio, and the distribution of myosin heavy chainIb, -IIa, fetal and embryonic isoforms did not differ between groups. Thus, even the relatively modest uterine crowding occurring naturally in CTR sows negatively affected placental and fetal development and the number of secondary muscle fibres. Consequences of more extreme crowding in utero on fetal and postnatal development, resulting from changing patterns of early embryonic survival, merit further investigation.

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“…1998). Furthermore, fetal erythropoiesis in Meishan gilts was accelerated compared to White crossbred gilts, suggesting that faster development of mature blood cells could be beneficial to fetal survival in swine (Vallet et al . 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Reports have suggested that uterine capacity may be influenced by fetal erythropoiesis (Pearson et al . 1998; Vallet et al . 2002, 2003).…”

Section: Introductionmentioning

confidence: 99%

A substitution within erythropoietin receptor gene D1 domain associated with litter size in Beijing Black pig, Sus scrofa

Zhang

Wang

et al. 2011

Animal Science Journal

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Studies of uterine capacity and litter size in swine have suggested that erythropoietin receptor (EPOR) plays an important role in fetal survival through maturation of red blood cells. In this study, we screened the porcine EPOR gene for mutations and identified three single nucleotide polymorphisms (SNPs): two missense mutations and one synonymous mutation. We then genotyped 272 Beijing Black sows, Sus scrofa, and compared this data with litter sizes from a total of 1523 parities among the sows. The G allele of the nonsynonymous SNP, EPOR c.434A>G, was associated with greater litter size at both first parity (P < 0.05) and at later parities (P < 0.01). This SNP causes His92Arg adjacent to the fourth conserved cysteine residue in the mature protein and is in the D1 domain of the protein. Additionally, we determined the allele frequencies for this SNP among six Chinese indigenous pig breeds (Bamei, Erhualian, Laiwu Black, Mashen, Meishan and Min) and three Western commercial pig breeds (Duroc, Landrace and Large White). The c.434G allele was significantly more common among the more prolific Chinese breeds than the Western breeds, implying that EPOR c.434A>G could be a useful genetic marker to improve litter size in swine.

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The effect of breed and intrauterine crowding on fetal erythropoiesis on day 35 of gestation in swine1

Cited by 17 publications

References 13 publications

Secreted and Placental Membrane Forms of Folate-Binding Protein Occur Sequentially During Pregnancy in Swine1

Secreted and Placental Membrane Forms of Folate-Binding Protein Occur Sequentially During Pregnancy in Swine1

Number of conceptuses in utero affects porcine fetal muscle development

A substitution within erythropoietin receptor gene D1 domain associated with litter size in Beijing Black pig, Sus scrofa

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