The Effect of Blood Flow-Restricted Low Resistance Training on Microvascular Circulation of Myocardium in Spontaneously Hypertensive Rats

Tan, Zhaowen; Zhao, Yan; Zheng, Yuchan; Pan, Ying

doi:10.3389/fphys.2022.829718

Cited by 4 publications

(8 citation statements)

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“…The resistance training mode was a gradually increasing load (the exercise groups received exercise intervention for 8 weeks starting at the age of 7 weeks) ( 17 ). The specific exercise plan is detailed in a previous study of the project team ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

“…At 1-min intervals, blood flow restriction was lifted, and blood flow was reperfused. After rest, the rubber band was used to bind the site again ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

“…The caudal artery blood pressure (BP) and heart rate (HR) of the rats at rest were measured using an intelligent non-invasive blood pressure tester BP-2000 (Ruanlong Biological Co., Beijing, China). Each rat was measured continuously three times, and the average value was used ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

“…After applying a 2–3% isoflurane anesthesia to the rats, a small animal high-frequency color ultrasound (Visual Sonics, Canada) was used to evaluate cardiac function in the five groups, including ejection fraction (EF), fractional shortening (FS), E peak/A peak of the mitral valve (MV E/A), interventricular septal thickness at end-diastole (IVSd), interventricular septal thickness at end-systole (IVSs), left ventricular internal diameter at end-diastole (LVIDd), left ventricular internal diameter at end-systole (LVIDs), left ventricular posterior wall end-diastolic thickness (LVPWd), left ventricular posterior wall end-systolic thickness (LVPWs), and left ventricular mass (LV mass). All rats were measured three times ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

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Effect of blood flow–restricted resistance training on myocardial fibrosis in early spontaneously hypertensive rats

Tan

Pei-you

Zheng

et al. 2023

Front. Cardiovasc. Med.

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ObjectiveThe purpose of this study was to explore the effect of blood flow–restricted resistance training on myocardial fibrosis in early spontaneously hypertensive rats (SHRs).MethodsFour-week-old male Wistar-Kyoto rats and SHRs were randomly divided into the following groups: normal group (WKY), SHR control (SHR-SED) group, high-intensity resistance training (HIRT) group, low- and medium-intensity resistance training (LMIRT) group, and blood flow–restricted low- and medium-resistance training (BFRT) group. Body weight, hemodynamics, cardiac function, myocardial morphology and fibrosis, and the expression levels of transforming growth factor-beta1-Smad (TGFβ-1-Smad) pathway-related proteins in the myocardium were assessed.Results(1) BFRT lowered blood pressure significantly, decreased left ventricular wall thickness, and improved cardiac function. At the same time, BFRT was superior to traditional resistance training in lowering diastolic blood pressure, and was superior to HIRT in improving left ventricular compliance, reducing heart rate, and reducing left ventricular posterior wall and left ventricular mass (P < 0.05). (2) BFRT decreased collagen I and collagen fiber area in the myocardium, increased the collagen III area, and decreased the collagen I/III ratio (P < 0.05). BFRT produced a better proportion of myocardial collagen fibers than did traditional resistance training (P < 0.05). (3) In the myocardium of the BFRT group compared to the traditional resistance training group, the expression of TGFβ-1, Smad2/3/4, p-Smad2/3, CTGF, and TIMP1 was significantly downregulated, MMP2 and TIMP2 were significantly upregulated, the MMP/TIMP ratio significantly increased, and TGFβ-1 expression significantly decreased (P < 0.05).ConclusionBFRT inhibited the TGFβ-1-Smad pathway in the myocardium, downregulated the expression of CTGF, and regulated the balance between MMPs and TIMPs, thereby reducing myocardial fibrosis in SHR, and improving cardiac morphology and function. BFRT also lowered blood pressure, and achieved an effect of early prevention and treatment of hypertension. At the same time, BFRT was superior to traditional resistance training in reducing diastolic blood pressure and adjusting the proportion of myocardial collagen fibers.

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Section: Methodsmentioning

confidence: 99%

“…At 1-min intervals, blood flow restriction was lifted, and blood flow was reperfused. After rest, the rubber band was used to bind the site again ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of blood flow–restricted resistance training on myocardial fibrosis in early spontaneously hypertensive rats

Tan

Pei-you

Zheng

et al. 2023

Front. Cardiovasc. Med.

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“…Resistance training models in rats most commonly involve tail-weighted ladder climbing or incline treadmill walking [17][18][19][20][21][22]51 . These options employ similar muscle activation to traditional strength training modalities (eg.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Model of Blood Flow Restriction in the Post-surgical Rat

Fosam,

Ramos Nakandakari,

Ohashi

et al. 2024

Preprint

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Blood flow restriction (BFR) with low-load exercise (BFR-exercise) is an increasingly popular tool used to increase muscle strength and attenuate muscle atrophy, especially after injury or surgery. However, the mechanisms underlying BFR-mediated muscle growth are not well understood. Likely contributing to the mechanistic knowledge gap, rodent models of BFR-exercise have not been well described. In this methods paper, we demonstrate a comprehensive, clinically relevant protocol to establish BFR-exercise in awake rats. This protocol includes generating a muscle loss state via bilateral ACL-R, determining targeted blood flow occlusion pressures, and performing weighted hind-limb knee extension exercises with BFR. These methods can be used for further application in mechanistic and physiologic studies of BFR-exercise.

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Impact of Blood Flow Restriction Exercise on Central Hemodynamics and Fluid Regulating Hormones

THOMPSON,

GAMBLE,

COATES

et al. 2023

Medicine &Amp; Science in Sports &Amp; Exercise

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Purpose To characterize both the efficacy of altered restriction pressures and consequences of optimized blood flow restriction (BFR) for altering the cardiovascular and fluid regulating response in humans. Methods This work comprised a series of related trials. Phase 1: during semi-recumbent cycling (5 min, 60 W) with BFR at 0, 50, 75, 100 and 125% of participants’ lowest arterial occlusion pressure (LOP) echocardiographic images were collected alongside continuous heart rate (HR) and blood pressure (BP). In phase 2, 24 h fluid balance (intake-output) and fluid-regulating hormone responses were measured after a practical BFR exercise session (treadmill walking: 5·3 min, 1 min rest, 5 km/h, 5% incline, 100% LOP). Phase 3 examined the magnitude and effect of blood volume distribution following BFR treadmill walking using a modified CO-rebreathe technique. Results Cardiac ejection fraction remained stable irrespective of cuff pressure and despite a reduction in end-diastolic volume (Δ of 11 ± 9 mL, p = 0.02). HR and BP were highest at 100% LOP (ΔHR = 18 ± 19 bpm, ΔSBP = 51 ± 30 mmHg, ΔDBP = 33 ± 15 mmHg, ΔRate Pressure Product = 76 ± 32 bpm·mmHg·100-1). BFR treadmill walking stimulated a greater release of fluid-regulating hormones than normal walking (Δrenin = BFR: 25.3 ± 24.2 vs. CON: 9.1 ± 11.8 ng/L; Δcopeptin = BFR: 50.1 ± 97.9 vs. CON: 0.43 ± 0.8 pmol/L), but no difference in fluid retention was observed (p = 0.2). Approximately 27% of total blood volume was confined to the legs during BFR, but the relative volume trapped in the legs was not related to fluid-regulating hormone release (renin r = -0.04, p = 0.9; copeptin r = 0.27, p = 0.3). Conclusions BFR exercise elicits the largest cardiovascular effect using 100% LOP, with evidence of appreciable fluid regulating hormonal response during a typical BFR exercise session. The relative volume of blood sequestered in the limb does not appear to drive this response.

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The Effect of Blood Flow-Restricted Low Resistance Training on Microvascular Circulation of Myocardium in Spontaneously Hypertensive Rats

Cited by 4 publications

References 53 publications

Effect of blood flow–restricted resistance training on myocardial fibrosis in early spontaneously hypertensive rats

Effect of blood flow–restricted resistance training on myocardial fibrosis in early spontaneously hypertensive rats

A Comprehensive Model of Blood Flow Restriction in the Post-surgical Rat

Impact of Blood Flow Restriction Exercise on Central Hemodynamics and Fluid Regulating Hormones

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