The effect of biomimetic calcium deficient hydroxyapatite and sintered β-tricalcium phosphate on osteoimmune reaction and osteogenesis

Sadowska, Joanna M.; Wei, Fei; Guo, Jia; Guillem-Marti, Jordi; Zhao, Lin; Ginebra, Maria‐Pau; Xiao, Yin

doi:10.1016/j.actbio.2019.06.057

Cited by 107 publications

(83 citation statements)

References 79 publications

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“…Pro-regenerative M2 cells are usually characterized by slender shapes. 14 In this study, the SEM images showed that on day 1, macrophages seeded on the Ti surface ( Figure 8A) showed a spherical and clustered morphology, which indicated the MΦ phenotype. Almost all of the LPS group's cells ( Figure 8B) were activated, and showed significantly rounded-like shapes, with enlargement of the spreading areas and signally stretched filopodia.…”

Section: Cell Morphology and Cytoskeleton Actin Stainingsupporting

confidence: 48%

“…In addition, immune cells including macrophages attracted to the site of implantation also play a crucial role in implant success by supplying a suitable immune microenvironment for bone regeneration around the implant. [13][14][15] Therefore, the effects of implant material on cell behaviors of macrophages, the important immune cell, have gradually attracted researchers' attention. [16][17][18][19] After the implant surgery, blood clots form around the implant through activation of the complement system.…”

Section: Introductionmentioning

confidence: 99%

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<p>Zn-Incorporated TiO2 Nanotube Surface Improves Osteogenesis Ability Through Influencing Immunomodulatory Function of Macrophages</p>

Chen¹,

You²,

Ma³

et al. 2020

IJN

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Purpose: Zinc (Zn), an essential trace element in the body, has stable chemical properties, excellent osteogenic ability and moderate immunomodulatory property. In the present study, a Zn-incorporated TiO 2 nanotube (TNT) was fabricated on titanium (Ti) implant material. We aimed to evaluate the influence of nano-scale topography and Zn on behaviors of murine RAW 264.7 macrophages. Moreover, the effects of Zn-incorporated TNT surface-regulated macrophages on the behaviors and osteogenic differentiation of murine MC3T3-E1 osteoblasts were also investigated. Methods: TNT coatings were firstly fabricated on a pure Ti surface using anodic oxidation, and then nano-scale Zn particles were incorporated onto TNTs by the hydrothermal method. Surface topography, chemical composition, roughness, hydrophilicity, Zn release pattern and protein adsorption ability of the Zn-incorporated TiO 2 nanotube surface were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), surface profiler, contact angle test, Zn release test and protein adsorption test. The cell behaviors and both pro-inflammatory (M1) and pro-regenerative (M2) marker gene and protein levels in macrophages cultured on Zn-incorporated TNTs surfaces with different TNT diameters were detected. The supernatants of macrophages were extracted and preserved as conditioned medium (CM). Furthermore, the behaviors and osteogenic properties of osteoblasts cultured in CM on various surfaces were evaluated. Results: The release profile of Zn on Zn-incorporated TNT surfaces revealed a controlled release pattern. Macrophages cultured on Zn-incorporated TNT surfaces displayed enhanced gene and protein expression of M2 markers, and M1 markers were moderately inhibited, compared with the LPS group (the inflammation model). When cultured in CM, osteoblasts cultured on Zn-incorporated TNTs showed strengthened cell proliferation, adhesion, osteogenesis-related gene expression, alkaline phosphatase activity and extracellular mineralization, compared with their TNT counterparts and the Ti group. Conclusion: This study suggests that the application of Zn-incorporated TNT surfaces may establish an osteogenic microenvironment and accelerate bone formation. It provided a promising strategy of Ti surface modification for a better applicable prospect.

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Section: Cell Morphology and Cytoskeleton Actin Stainingsupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

<p>Zn-Incorporated TiO2 Nanotube Surface Improves Osteogenesis Ability Through Influencing Immunomodulatory Function of Macrophages</p>

Chen¹,

You²,

Ma³

et al. 2020

IJN

View full text Add to dashboard Cite

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“…Mineral composites are particularly critical for the osteogenesis process in vivo. Specifically, calcium deficient hydroxyapatite (dHA) induces a superior calcium and phosphate decrease, triggering a cascading osteoinductive effect that involves a fine-tuned immunoresponse [156]. Mineral/biomaterials composites having the benefit of the osteoinductive potential (from the mineral component) have superior biocompatibility (induced by the biopolymer); this double feature favours the cross-talk between the substrate and the mammalian cell.…”

Section: Bioscaffolds For Cell Cultivation In Vitromentioning

confidence: 99%

Recent Advances in Bioplastics: Application and Biodegradation

et al. 2020

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The success of oil-based plastics and the continued growth of production and utilisation can be attributed to their cost, durability, strength to weight ratio, and eight contributions to the ease of everyday life. However, their mainly single use, durability and recalcitrant nature have led to a substantial increase of plastics as a fraction of municipal solid waste. The need to substitute single use products that are not easy to collect has inspired a lot of research towards finding sustainable replacements for oil-based plastics. In addition, specific physicochemical, biological, and degradation properties of biodegradable polymers have made them attractive materials for biomedical applications. This review summarises the advances in drug delivery systems, specifically design of nanoparticles based on the biodegradable polymers. We also discuss the research performed in the area of biophotonics and challenges and opportunities brought by the design and application of biodegradable polymers in tissue engineering. We then discuss state-of-the-art research in the design and application of biodegradable polymers in packaging and emphasise the advances in smart packaging development. Finally, we provide an overview of the biodegradation of these polymers and composites in managed and unmanaged environments.

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“…The release of IL-6, an inflammatory cytokine, significantly increased with OCP treatment for 12 h compared to that of HL. The chemical composition of Ca-P also affects the osteoimmune response and osteogenesis 127) . Macrophages (Raw264.7) were cultured on CDHA and β-TCP to assess the release of inflammatory cytokines and osteogenic factors.…”

Section: Macrophagesmentioning

confidence: 99%

Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials

2020

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Octacalcium phosphate (OCP) is a material that can be converted to hydroxyapatite (HA) under physiological environments and is considered a mineral precursor to bone apatite crystals. The structure of OCP consists of apatite layers stacked alternately with hydrated layers, and closely resembles the structure of HA. The performance of OCP as a bone substitute differs from that of HA materials in terms of their osteoconductivity and biodegradability. OCP manifests a cellular phagocytic response through osteoclastlike cells similar to that exhibited by the biodegradable material β-tricalcium phosphate (β-TCP). The use of OCP for human cranial bone defects involves using its granule or composite form with one of the natural polymers, viz., the reconstituted collagen. This review article discusses the differences and similarities in these calcium phosphate (Ca-P)-based materials from the viewpoint of the structure and their material chemistry, and attempts to elucidate why CaP materials, particularly OCP, display unique osteoconductive property.

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The effect of biomimetic calcium deficient hydroxyapatite and sintered β-tricalcium phosphate on osteoimmune reaction and osteogenesis

Cited by 107 publications

References 79 publications

<p>Zn-Incorporated TiO2 Nanotube Surface Improves Osteogenesis Ability Through Influencing Immunomodulatory Function of Macrophages</p>

<p>Zn-Incorporated TiO2 Nanotube Surface Improves Osteogenesis Ability Through Influencing Immunomodulatory Function of Macrophages</p>

Recent Advances in Bioplastics: Application and Biodegradation

Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials

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