Students and the public have a negative perception or dislike of herpetofauna. The solution to this problem is to introduce herpetofauna through mobile learning. The research aimed to determine the effect of mobile based learning towards attitudes and knowledge about herpetofauna in prospective biology teachers. The research method used Pre-experimental Design with the form of research one-group Pretest Posttest Design. Attitude data was collected using questionnaires and knowledge using tests. The data obtained were analyzed using the Wilcoxon Signed Rank Test. The research results obtained asymp. Sig 0.00 < 0.05. There are differences in attitudes between men and women. It can be concluded that mobile based learning to enhance attitudes and knowledge about herpetofauna in prospective biology teachers.