The Effect of Benzyl Isothiocyanate on the Expression of Genes Encoding NADH Oxidase and Fibronectin-Binding Protein in Oral Streptococcal Biofilms

Alhandal, Hawraa; Almesaileikh, Esraa; Bhardwaj, Radhika G.; Khabbaz, Areej Al; Karched, Maribasappa

doi:10.3389/froh.2022.863723

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…Sofrata et al [43] reported a strong antibacterial efficacy of S. persica root chewing sticks against gram-negative oral pathogens due to the presence of benzyl isothiocyanate. Benzyl isothiocyanate, an active component of S. persica, is a well-known bactericidal material that can restrict acid production and the growth of many oral pathogens [44]. The presence of essential oils, sulfur, potassium chloride, sodium chloride, and some organic compounds contribute to its antimicrobial properties [45].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Green Synthesis of Nickel Nanoparticles Using Salvadora persica and Their Application in Antimicrobial Activity against Oral Microbes

Balto,

Amina,

Bhat

et al. 2023

Microbiology Research

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The current study evaluated the biogenic synthesis of nickel oxide nanoparticles (SP-NiONPs) from the root extract of (Salvadora persica) S. persica and their biological properties. The nanoparticles were characterized using spectroscopic and microscopic techniques and then evaluated for their antimicrobial properties against 10 oral pathogens. The ultraviolet-visible (UV–Vis) spectra exhibited a distinctive resonance spectrum at 334 nm for the SP-NiONPs produced from S. persica. The fourier transform infrared (FTIR) analysis revealed the presence of functional groups of biomolecules of S. persica that served as reducing and capping agents of the SP-NiONPs. The scanning electron microscope (SEM) and transmission electron microscopy (TEM) analyses showed that the nanoparticles were spherical-shaped, tightly packed, and ranged in size from 18.20 nm to 45.12 nm. The energy dispersive x-ray (EDX) analysis confirmed 69.9% of the nickel (Ni) content by weight, and the X-ray diffraction (XRD) results showed the face-centered cubic (FCC) crystalline structure of the formed SP-NiONPs. The antioxidant activity of the SP-NiONPs exhibited a dose-dependent profile with an IC50 value of 51.45 ± 0.65 and a 54.13 ± 0.98 DPPH• and ABTS•+ radical scavenging activity, respectively. The SP-NiONPs showed an antibacterial activity against all the test strains; however, E. cloacae was found to be the most sensitive strain, with an inhibition zone of 31 ± 0.50 mm. The SEM image of the E. cloacae cells treated with SP-NiONPs showed irregular shapes and ruptured, destroyed cell membranes. Our findings revealed that SP-NiONPs could be used as excellent antibacterial agents against oral pathogens.

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Section: Antimicrobial Activitymentioning

confidence: 99%

Green Synthesis of Nickel Nanoparticles Using Salvadora persica and Their Application in Antimicrobial Activity against Oral Microbes

Balto,

Amina,

Bhat

et al. 2023

Microbiology Research

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Anti-microbial activity and biofilm inhibition of Streptococcus mutans by Coriandrum sativum essential oil and isothiocyanates

Ngalah

Hansen

Frank³

et al. 2023

Preprint

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Aims This study evaluated the antimicrobial effect of coriander oil (CO) and isothiocyanates (ITC; Allyl, Benzyl and Phenyl-A-, B-, P-ITC) on clinical oral isolate Streptococcus mutans. We evaluated inhibition of biofilm, metabolic activity and exopolysaccharide formation. Methods The composition of CO was determined by gas chromatography- mass spectrometry (GC-MS). CO and ITC were dissolved in 0.1% Tween 80. Different concentrations (0.0039-1%) of CO, ITC and CO-ITC were made in Tryptic soy broth (TSB) and with sucrose (TSBS). Broth dilution method was used to determine the minimum inhibitory concentration (MIC). Minimum biofilm inhibitory concentrations (MBIC) were established by staining biofilm with crystal violet. Checkerboard assay was used to evaluate combination effects. An XTT assay was used to determine the metabolic activity and a sulphuric acid-phenol assay for inhibition of S. mutans exopolysaccharide production. Results CO was dominated by Linalool (65.5%) followed by 2-bornanone (6.16%) and Gamma-terpinene (4.31%). The least was Terpinen-4-ol (0.13%). The MIC of CO was 0.00195% and < 0.00195% for ITCs. MBIC for B-ITC was at < 0.0039%, while with CO-ITC at 0.00195% (p < 0.05). The MBC of CO was 0.125% and for CO-B-ITC MBIC was < 0.00195%. The B-ITC MBC was > 0.25%. Strong S. mutans exopolysaccharide inhibition was observed with ITC and CO at 0.0039%. CO reduced S. mutans metabolic activity at 0.125% and ITC at 0.0078%. Conclusion and clinical relevance: CO and ITC are promising agents in prevention of both periodontal disease and dental caries. Further data is required to warrant development of products to help mitigate dental caries and periodontal disease.

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The Effect of Benzyl Isothiocyanate on the Expression of Genes Encoding NADH Oxidase and Fibronectin-Binding Protein in Oral Streptococcal Biofilms

Cited by 2 publications

References 34 publications

Green Synthesis of Nickel Nanoparticles Using Salvadora persica and Their Application in Antimicrobial Activity against Oral Microbes

Green Synthesis of Nickel Nanoparticles Using Salvadora persica and Their Application in Antimicrobial Activity against Oral Microbes

Anti-microbial activity and biofilm inhibition of Streptococcus mutans by Coriandrum sativum essential oil and isothiocyanates

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