The effect of benzo(a)pyrene on porcine urinary bladder epithelial cells analyzed for the expression of selected genes and cellular toxicological endpoints

Wolf, Alexander; Kutz, Alexandra; Plöttner, Sabine; Behm, Claudia; Bolt, Herrmann; Föllmann, Wolfram; Kuhlmann, Jürgen

doi:10.1016/j.tox.2004.09.006

Cited by 29 publications

(18 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1a). As known from previous studies (Wolf et al 2005;Plöttner et al 2008a) 1 M B[a]P caused a clear induction of CYP1A1 mRNA expression. Interestingly, 4-ABP caused a further increase of B[a]Pinduced enzyme induction (Fig.…”

Section: Resultssupporting

confidence: 74%

“…The cells were cultured under serum-free conditions according to Guhe and Föllmann (1994), with minor modiWcations as described earlier (Wolf et al 2005;Plöttner et al 2008a). Urinary bladders were obtained from slaughtered pigs immediately after opening of the abdominal wall about 15-20 min after slaughter at the local slaughterhouse of Lünen, Germany.…”

Section: Methodsmentioning

confidence: 99%

“…PUBEC were seeded in 96-well microtiter plates in serum-free medium, and evaluated as described previously (Föllmann et al 2000b(Föllmann et al , 2007Wolf et al 2005). In brief, 20,000 PUBEC in 200 l culture medium were seeded per well in 96-well culture plates.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

“…Real-time RT-PCR with on-line detection of Xuorescence was performed with a Lightcycler 3 instrument (Roche, Mannheim, Germany). QuantiWcation of CYP1A1, UGT1 and COX-2 was carried out by one-step RT-PCR as previously described in detail (Wolf et al 2005). Three hundred nanograms of total RNA and 6 nmol of gene speciWc primers were used in a 20 l reaction (DNA Master SybrGreen I Mix, Roche) in glass vials (Roche, Mannheim, Germany).…”

Section: Real-time Reverse Transcription Pcr (Rt-rt-pcr)mentioning

confidence: 99%

See 3 more Smart Citations

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

et al. 2008

Self Cite

View full text Add to dashboard Cite

Aromatic amines have been shown to cause bladder cancer. However, epithelial cells of the urinary bladder, cells of origin of bladder cancer, may be exposed to numerous substances besides aromatic amines. In the present study, we analysed possible interactions between the aromatic amines 4-aminobiphenyl (4-ABP) as well as 2-naphthylamine (2-NA) and the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P). For this purpose we incubated primary porcine urinary bladder epithelial cells (PUBEC) with concentrations of 1 to 50 M 4-ABP with and without coexposure to B[a]P. As expected B[a]P increased mRNA expression of cytochrome P450 1A1 (CYP1A1), whereas 4-ABP had no eVect. However, when co-exposed 4-ABP enhanced the induction of CYP1A1 by B[a]P. This result was conWrmed by Western blot analysis of CYP1A1 protein expression. A similar eVect as for CYP1A1 was also observed for cyclooxygenase-2 (COX-2) and UDP-glucuronosyltransferase 1 (UGT1). Next, we studied co-exposures of 2-NA and B[a]P. Similar as for 4-ABP also 2-NA enhanced B[a]P-mediated induction of CYP1A1. Our results demonstrate that some aromatic amines may enhance the inXuence of B[a]P on Ah receptor-dependent genes.

show abstract

Section: Resultssupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Cytotoxicity Assaymentioning

confidence: 99%

Section: Real-time Reverse Transcription Pcr (Rt-rt-pcr)mentioning

confidence: 99%

See 2 more Smart Citations

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…A study conducted in human BC by Murai et al (Murray et al, 1995) demonstrated an increased expression of CYP1A1 using immunohistochemistry. An in vitro investigation carried out by Wolf et al (Wolf et al, 2005) found that cells treated with benzo [a] pyrene showed a dramatic increase in the expression of CYP1A1. Similar conclusions were also found in other experimental animals whose bladder cells were exposed to tobacco containing compounds such as benzo[a]pyrene and arylamines (Kogevinas et al, 2003;Dorrenhaus et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Lack of Association Between CYP1A1 Polymorphisms and Risk of Bladder Cancer: a Meta-analysis

Zhang²,

Xie³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: The effects of CYP1A1 gene polymorphisms on the risk of bladder cancer (BC) remain controversial. We carried out a meta-analysis to clarify the role of CYP1A1 gene polymorphisms in BC. Material and Methods: A comprehensive literature search was conducted up to November 20, 2013. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to estimate the strength of the association. Meta-regression, subgroup analysis, sensitivity analysis and publication bias were also performed. Results: Eight studies involving 1,059 BC cases and 1,061 controls were included. The meta-analysis showed that there was no significant association between the two common mutations of CYP1A1 and BC risk. For the I1e462Val A/G polymorphism with GG vs. AA the OR was 1.47 (95 % CI= 0.70-3.07, P =0.308). For the MspI T/C polymorphism, though a slight trend was found this was not statistically nonsignificant (CC vs.TT, OR = 1.24, 95 % CI= 0.98-1.58, P =0.078). Subgroup analyses by ethnicity also found no obvious association between CYP1A1 and BC risk. Conclusion: The present meta-analysis suggests that CYP1A1 polymorphism is not associated with bladder cancer risk.

show abstract

Chronic and permanent injury

Coleman

2014

Expert Report Writing in Toxicology

View full text Add to dashboard Cite

The effect of benzo(a)pyrene on porcine urinary bladder epithelial cells analyzed for the expression of selected genes and cellular toxicological endpoints

Cited by 29 publications

References 42 publications

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

Lack of Association Between CYP1A1 Polymorphisms and Risk of Bladder Cancer: a Meta-analysis

Chronic and permanent injury

Contact Info

Product

Resources

About