The effect of bacteriospermia and leukocytospermia on conventional and nonconventional semen parameters in healthy young normozoospermic males

Frączek, Monika; Hryhorowicz, Magdalena; Gill, Kamil; Zarzycka, Marta; Gączarzewicz, Dariusz; Jȩdrzejczak, Piotr; Bilińska, Barbara; Piasecka, Małgorzata; Kurpisz, Maciej

doi:10.1016/j.jri.2016.08.006

Cited by 61 publications

(79 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Imamoğlu et al [ 46 ] demonstrated an increase in MDA concentration in the blood of cryptorchid boys. When interpreting the results obtained in this study, it is necessary to explain that only a small number of products of lipid sperm membrane peroxidation is shed to seminal plasma, while the remainder is bound to sperm membranes [ 47 ]. Therefore, it cannot be excluded that intracellular MDA levels, which were not determined in this or in other studies, were higher in the examined samples of men exposed to local heat stress.…”

Section: Discussionmentioning

confidence: 99%

Seminal Plasma Analysis of Oxidative Stress in Different Genitourinary Topographical Regions Involved in Reproductive Tract Disorders Associated with Genital Heat Stress

Frączek

Wojnar

Kamieniczna

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The pathophysiological mechanisms responsible for male subfertility/infertility caused by or complicated by genital heat stress remains unclear in many respects. Because seminal plasma creates the environment for the proper functioning of spermatozoa, in this study, we verified the associations among standard spermiograms, seminal biochemical parameters (neutral alpha-glucosidase, fructose, and citric acid) and oxidative stress markers (total antioxidant capacity, catalase activity, superoxide dismutase activity, and malondialdehyde concentration) in distinct entities associated with male infertility with and without long-time exposure to local hyperthermia. We demonstrated that men exposed to prolonged environmental or clinically recognized local heat stress in adulthood may suffer from dysregulation of seminal antioxidant components, which can be directly associated with epididymal and prostate function. The comparative analysis of the studied parameters showed numerous correlations among all biochemical parameters (particularly neutral alpha-glucosidase) with low standard semen quality in almost all the investigated infertile groups. In light of the data obtained in this originally designed study, we conclude that more attention should be paid to the epididymis and accessory gland function in subfertile and infertile men exposed to genital heat stress, especially in the context of novel treatment algorithms (targeted therapies).

show abstract

Section: Discussionmentioning

confidence: 99%

Seminal Plasma Analysis of Oxidative Stress in Different Genitourinary Topographical Regions Involved in Reproductive Tract Disorders Associated with Genital Heat Stress

Frączek

Wojnar

Kamieniczna

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the rst stage, bacterial infection can be induced by microorganisms, and pathological bacterial strains can be observed in semen, without attracting a large number of white blood cells; in the second stage, activated white blood cells appear in ejaculation; in the third stage, bacteria are removed, usually with isolated leukocytospermia as the representative [21]. Bacteria are mainly involved in the intrinsic and mitochondrial dependent apoptosis cell death, while oxidative stress may play a role in reducing the conventional sperm parameters in leukocytospermia, and the combined effect of bacteria and leukocytes accelerated the apoptosis and necrosis of sperm [22]. The increased seminal leukocytes may mediate reactive oxygen species (ROS) production by direct cell-cell contact or soluble products released by leukocytes [23].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Screening and Treatment of Ureaplasma Urealyticum Infection on Semen Parameters in Asymptomatic Leukocytospermia: A Case Control Study

Zhang¹,

Zhang

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Ureaplasma urealyticum (UU) infection, as well as asymptomatic leukocytospermia, whether it has effect on semen parameters and whether it needs screening and treatment is still a confusing and controversial topic for clinicians.Methods: Among 1,530 adult males who visited Guilin People's Hospital due to infertility, 295 were diagnosed with asymptomatic leukocytospermia, and 95 were further screened for UU-positive. 81 UU-positive asymptomatic leukocytospermia patients received 7-day or 14-day treatment plan with doxycycline, and 70 cases were cured. The semen parameters of non-leukocytospermia, leukocytospermia, UU-positive leukocytospermia and UU-negative leukocytospermia groups were compared, and the differences between the two treatment plans and the semen parameters before UU treatment and 1 month after UU-cured were compared.Results: Compared with non-leukocytospermia patients, the sperm concentration, progressive motility (PR), and normal morphology of patients with leukocytospermia decreased, while those with UU-positive leukocytospermia performed more significantly. The PR, total motility, and normal morphology of UU-positive leukocytospermia patients were significantly lower than those of UU-negative leukocytospermia patients (all p<0.001). The UU cure rates of the 7-day and 14-day treatment plan with doxcycline was 84.62% and 89.66% (p=0.738), respectively, and the sperm concentration, PR, total motility, and normal morphology of the cured UU-positive leukocytospermia patients were all increased after 1 month (p=0.001, p=0.022, p=0.004 and p=0.008, respectively).Conclusions: It is significant to screen and treat UU infection in asymptomatic leukocytospermia for improving sperm quality. Where appropriate, the 7-day treatment plan with doxycycline may be a good choice.

show abstract

“…Leucocytospermia is widely used as diagnostic marker in GTI. And, the overall number of seminal WBC is one of the screening indexes for genital tract infection and/or inflammation (Fraczek et al., ; Wang, Politch, & Anderson, ). In addition to the peroxidase test recommended by the WHO to detect polymorphonuclear (PMN) granulocytes, there are still other methods such as computer‐assisted semen analysis (CASA) and flow cytometery (Dearing et al., ; Fathy et al., ), which are employed for seminal WBC analysis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Sysmex UF-1000i system as an alternative for the screening of genital tract inflammation in male infertility patients

Wang

et al. 2018

Andrologia

View full text Add to dashboard Cite

The number of white blood cell (WBC) in semen is an important indicator of genital tract inflammation in male infertility. The peroxidase assay is the recommended reference method for seminal WBC counting. However, it is time-consuming and may cause relatively heavy workload in daily routine. Meanwhile, the main component in the reagent of peroxidase test is harmful to human and the environment. In this study, we evaluated the analytical performance of the Sysmex UF-1000i that is a urine flow cytometer as a screening tool for genital tract infection in male infertility patients through the counting of seminal WBC. We examined 143 semen samples and compared the results of UF-1000i and manual microscopy. The intra-assay variability, stability and linearity studies were performed. The intravariability (CV %) of seminal WBC count by Sysmex UF-1000i was 2.34%-9.65%. The method of UF-1000i displayed a good agreement with the reference assay of manual microscopy, and the r value for correlation of seminal WBC count between UF-1000i and manual microscopy was over 0.999 (p < 0.001). The Sysmex UF-1000i is capable of producing reliable seminal WBC count consistent with that obtained by manual microscopy. It is a suitable alternative to the manual microscopy, thus reduces the workload.

show abstract

The effect of bacteriospermia and leukocytospermia on conventional and nonconventional semen parameters in healthy young normozoospermic males

Cited by 61 publications

References 40 publications

Seminal Plasma Analysis of Oxidative Stress in Different Genitourinary Topographical Regions Involved in Reproductive Tract Disorders Associated with Genital Heat Stress

Seminal Plasma Analysis of Oxidative Stress in Different Genitourinary Topographical Regions Involved in Reproductive Tract Disorders Associated with Genital Heat Stress

The Effect of Screening and Treatment of Ureaplasma Urealyticum Infection on Semen Parameters in Asymptomatic Leukocytospermia: A Case Control Study

Evaluation of the Sysmex UF-1000i system as an alternative for the screening of genital tract inflammation in male infertility patients

Contact Info

Product

Resources

About