The effect of B vitamin supplementation on wound healing in type 2 diabetic mice

Mochizuki, Saeka; Takano, M.; Sugano, Naoyuki; Ohtsu, Mariko; Tsunoda, Kou; Koshi, Ryosuke; Yoshinuma, Naoto

doi:10.3164/jcbn.14-122

Cited by 12 publications

(6 citation statements)

References 32 publications

(28 reference statements)

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“…All mice were fed NIH-31 open formula diet containing 2 mg FA per kg diet (Xietong Pharmaceutical Biotechnology Co., Ltd., Jiangsu, China), which is generally recommended as the basal dietary requirement for mice (Reeves, 1997). At 8 weeks of age, 6 male (2 male per cage) and 12 female (4 female per cage) mice were randomly divided into three groups and given with de-ionized water (Qipshidze et al, 2011; Mochizuki et al, 2016) containing no FA (control), 3.75 mg/L FA (F7876, Sigma-Aldrich, St. Louis, MO, United States) or 22.5 mg/L FA for 1 week. Then 2 females were caged with 1 male and allowed to mate naturally.…”

Section: Methodsmentioning

confidence: 99%

Excess Folic Acid Supplementation Before and During Pregnancy and Lactation Activates Fos Gene Expression and Alters Behaviors in Male Mouse Offspring

Chu

Jiang

et al. 2019

Front. Neurosci.

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Periconceptional folic acid (FA) supplementation is recommended to prevent neural tube defects and other birth defects. After 20 years mandate food fortification with FA, serum concentration of folate and unmetabolized FA increased significantly in the North American population. But whether excess FA intake impairs neurodevelopment and behavior is still controversial. Here, we treated mice with approximately 2.5-fold (moderate dose of FA, MFA) or 10-fold (high dose of FA, HFA) the dietary requirement of FA 1 week before mating and throughout pregnancy and lactation, and examined behaviors in adult male offspring using open field test, three-chamber sociability and social novelty test, elevated plus maze, rotarod and Morris water maze. We found that early life MFA supplementation increased long-term body weight gain in adults, elevated anxiety-like behavior, and impaired social preference, motor learning and spatial learning ability without modifying motor ability and spatial memory. In contrast, HFA supplementation only induced mild behavioral abnormality. RNA sequencing revealed that FA supplementation altered the expression of brain genes at weaning, among which Fos and related genes were significantly up-regulated in MFA mice compared with control and HFA mice. Quantitative real time-PCR (qRT-PCR) and western blots confirmed the increase of these genes. Our results suggested that FA supplementation during early life stage affected gene expression in weaning mice, and exhibited long-term impairments in adult behaviors in a dose-sensitive manner.

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Section: Methodsmentioning

confidence: 99%

Excess Folic Acid Supplementation Before and During Pregnancy and Lactation Activates Fos Gene Expression and Alters Behaviors in Male Mouse Offspring

Chu

Jiang

et al. 2019

Front. Neurosci.

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“…The relationship between B12, folic acid levels and RT side effects is also a current research topic. These vitamins are important factors in DNA metabolism and wound healing (27). It is possible that there is a relationship between acute and chronic tissue damage due to radiation and vitamin values.…”

Section: Discussionmentioning

confidence: 99%

Factors on development and severity of acute radiodermatitis: prospective single-center study

İnan

Arslan

Aral

et al. 2022

Anatolian Current Medical Journal

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Aim: Although prior literature has examined the treatment and patient-associated factors affecting the development and severity of acute radiodermatitis, there are relatively few prospective studies evaluating both. This study was prospectively designed to evaluate factors affecting the development and extent of radiation-induced acute skin toxicity called radiodermatitis (RD). Material and Method: A total of 63 patients who underwent radiotherapy (RT) in Ankara Atatürk Research and Education Hospital between July 2017 and October 2018 were evaluated. Patients’ demographic status, disease/treatment details, hemoglobin, ferritin, folic acid, Vit B12, and hemoglobin A1c values were recorded. The development and grade of RD were evaluated weekly by the same radiation oncologist using the Radiation Therapy Oncology Group (RTOG) radiation toxicity guideline. Results: There was no significant relationship between the development of any degree of RD and gender, concomitant chemotherapy (CT), pre-RT CT, comorbid disease, RT technique and blood parameters (Hb, Hba1c, ferritin, folic acid and B12). The development of grade 2-3 RD was significantly affected by the number of operations (p=0.032) and total dose of RT (p=0.008). In patients with grade 2/3 RD, the RT dose at which RD first appeared was 20 Gy (range, 14-36); in patients with grade 1 RD, this value was 32 Gy (range, 16-56) (p=0.018). Conclusion: There is no significant relationship between the development of acute radiodermatitis and Hba1c, hemoglobin, ferritin, B12 and folic acid levels. There was a significant correlation between grade of RD and repeated surgery, increase in total RT dose and early onset of RD.

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“…Formula of the diet is supplied in Supplementary Table S1 . At 8 weeks of age, 4 males and 8 females were randomly divided into two groups and supplied with no (control) or 3.75 mg/L FA (Sigma-Aldrich, St. Louis, MO, USA) in their drinking water for one week [ 18 , 21 , 22 ]. Then, the mice were allowed to mate naturally by introducing 2 females in the cage of 1 male.…”

Section: Methodsmentioning

confidence: 99%

Excess Folic Acid Supplementation before and during Pregnancy and Lactation Alters Behaviors and Brain Gene Expression in Female Mouse Offspring

Yang

Sun

et al. 2021

Nutrients

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Use of folic acid (FA) during early pregnancy protects against birth defects. However, excess FA has shown gender-specific neurodevelopmental toxicity. Previously, we fed the mice with 2.5 times the recommended amount of FA one week prior to mating and during the pregnancy and lactation periods, and detected the activated expression of Fos and related genes in the brains of weaning male offspring, as well as behavioral abnormalities in the adults. Here, we studied whether female offspring were affected by the same dosage of FA. An open field test, three-chamber social approach and social novelty test, an elevated plus-maze, rotarod test and the Morris water maze task were used to evaluate their behaviors. RNA sequencing was performed to identify differentially expressed genes in the brains. Quantitative real time-PCR (qRT-PCR) and Western blots were applied to verify the changes in gene expression. We found increased anxiety and impaired exploratory behavior, motor coordination and spatial memory in FA-exposed females. The brain transcriptome revealed 36 up-regulated and 79 down-regulated genes in their brains at weaning. The increase of Tlr1; Sult1a1; Tph2; Acacb; Etnppl; Angptl4 and Apold1, as well as a decrease of Ppara mRNA were confirmed by qRT-PCR. Among these genes; the mRNA levels of Etnppl; Angptl4andApold1 were increased in the both FA-exposed female and male brains. The elevation of Sult1a1 protein was confirmed by Western blots. Our data suggest that excess FA alteres brain gene expression and behaviors in female offspring, of which certain genes show apparent gender specificity.

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The effect of B vitamin supplementation on wound healing in type 2 diabetic mice

Cited by 12 publications

References 32 publications

Excess Folic Acid Supplementation Before and During Pregnancy and Lactation Activates Fos Gene Expression and Alters Behaviors in Male Mouse Offspring

Excess Folic Acid Supplementation Before and During Pregnancy and Lactation Activates Fos Gene Expression and Alters Behaviors in Male Mouse Offspring

Factors on development and severity of acute radiodermatitis: prospective single-center study

Excess Folic Acid Supplementation before and during Pregnancy and Lactation Alters Behaviors and Brain Gene Expression in Female Mouse Offspring

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