The effect of Astragalus polysaccharide on the Epstein-Barr virus lytic cycle

Abstract

Summary. -Effects of a polysacharide from Chinese herbal plant Astragalus membranaceus (APS) on the expression of Epstein-Barr virus (EBV) immediate early proteins Zta, Rta and EA-D in Raji cells were examined. EBV switch from latent to lytic cycle in Raji cells was induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) and sorbol butyrate (SB) and the effects of APS were examined by immunofluorescence, western blotting and flow cytometry. APS in a non-cytotoxic concentration of 30 μg/ml significantly suppressed the expression of Zta, Rta and EA-D during the EBV lytic cycle. Our observations indicate that APS is potentially useful as an anti-EBV drug.

Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway

“…It has been reported that APS in a non-cytotoxic concentration of 30 g/mL significantly suppressed the expression of immediate early proteins during the EBV lytic cycle, indicating that APS is potentially useful as an anti-EBV drug [30]. APS protects astrocytes from being infected by HSV-1, indicating that APS inhibit viral infections.…”

A Review of the Pharmacological Action of Astragalus Polysaccharide

“…In addition, it effectively inhibited the cytopathic changes induced by coxsackievirus B3 and the proliferation of the virus in Vero cells and cardiomyocytes (Liu et al, 2003). It was also found that APS at a non-cytotoxic concentration of 30 mg/ml significantly inhibited the expression of zipper transcription factor, transcription activator, and the diffuse component of early antigen in the Epstein-Barr virus cleavage cycle, showing that APS carries potential as an anti-Epstein-Barr virus drug (Guo et al, 2014). Moreover, APS inhibited the replication of infectious bronchitis virus in vitro.…”

RETRACTED ARTICLE: Astragalus polysaccharide alleviates H 2 O 2 -triggered oxidative injury in human umbilical vein endothelial cells via promoting KLF2

“…APS is a sort of effective components of Astragalus membranaceus, which has a wide range of pharmacological effects [29]. At present, most researches about APS focus on the protection of cardiac myocytes, and the anti-oxidant effect of APS has been reported.…”