The effect of ascorbic acid during biopsy and cryopreservation on viability of bovine embryos produced in vivo

Korhonen, Kati; Julkunen, Heikki; Kananen, Kirsi; Bredbacka, Peter; Tiirikka, Timo; Räty, Mervi; Vartia, K.; Kaimio, Iris; Kontinen, A.; Halmekytö, Maria; Vilkki, Johanna; Peippo, Jaana; Lindeberg, Heli

doi:10.1016/j.theriogenology.2011.07.034

Cited by 6 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, this is the first report evaluating the effect of the addition of ascorbate to freezing medium on cryosurvival of bovine IVP embryos. However, the finding that ascorbate treatment increased post-thaw cryotolerance is consistent with previous reports involving in vivo derived bovine embryos (Korhonen et al, 2011) and murine (Lane et al, 2002) and porcine (Castillo-Martín et al, 2014a, b, 2015 embryos produced by IVP. Results of the present study indicate that ascorbate improves post-thaw cryosurvival, at least in part, by ameliorating the impact of oxidative stress induced by the freezing and thawing process.…”

Section: Discussionsupporting

confidence: 92%

“…Furthermore, the supplementation of vitrification and warming solutions with ascorbic acid improved the cryosurvival of porcine IVP embryos (Castillo‐Martín et al, 2014a, b, 2015). Studies with ascorbate and bovine embryo cryopreservation are limited; yet, treatment of in vivo derived embryos with ascorbate during biopsy and cryopreservation improved calving rates following embryo transfer (Korhonen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the supplementation of vitrification and warming solutions with ascorbic acid improved the cryosurvival of porcine IVP embryos (Castillo-Martín et al, 2014a, b, 2015. Studies with ascorbate and bovine embryo cryopreservation are limited; yet, treatment of in vivo derived embryos with ascorbate during biopsy and cryopreservation improved calving rates following embryo transfer (Korhonen et al, 2011). Dithiothreitol (DTT), an antioxidant that acts as a strong reducing agent and contains two thiol groups for sequential thioldisulfide exchange reactions for direct scavenging of ROS (Rothwarf & Scheraga, 1992), can also protect cells against oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of addition of ascorbate, dithiothreitol or a caspase‐3 inhibitor to cryopreservation medium on post‐thaw survival of bovine embryos produced in vitro

Carrascal-Triana

Zolini

King

et al. 2022

Reprod Domestic Animals

View full text Add to dashboard Cite

Experiments were conducted to investigate whether supplementation of cryopreservation medium with ascorbate, dithiothreitol (DTT) or an inhibitor of caspase-3 (z-DEVD-fmk) could improve post-thaw survival of bovine embryos produced in vitro (IVP). For all experiments, embryos were harvested on day 7 after insemination and subjected to controlled-rate freezing in medium containing 1.5 M ethylene glycol and treatments as described below. In experiments 1-3, embryos were cryopreserved in freezing medium with ascorbate (0, 0.1, 0.3 or 0.5 mM), DTT (0, 50, 100 or 200 μM) and z-DEVD-fmk (0, 50, 100 or 200 μM), respectively. Post-thaw survival was assessed at 24, 48 and 72 h. For experiments 4-5, embryos were cryopreserved in freezing medium with or without 0.1 mM ascorbate. At 24 h post-thaw, embryo total cell number, DNA fragmentation and levels of reactive oxygen species (ROS) were evaluated.Embryos subjected to freezing and thawing in medium supplemented with 0.1 mM ascorbate had greater (p < .05) re-expansion rates at 24, 48 and 72 h and hatching rate at 72 h as compared to embryos not treated with ascorbate. Post-thaw cryosurvival was not affected by the addition of either DTT or z-DEVD-fmk to medium used for cryopreservation. Embryos cryopreserved in medium supplemented with 0.1 mM ascorbate had reduced (p < .001) levels of intracellular ROS and fewer (p < .001) cells with DNA fragmentation. In conclusion, post-thaw survival of bovine IVP embryos is enhanced by supplementation of freezing medium with ascorbate.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of addition of ascorbate, dithiothreitol or a caspase‐3 inhibitor to cryopreservation medium on post‐thaw survival of bovine embryos produced in vitro

Carrascal-Triana

Zolini

King

et al. 2022

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The increase in the parameters of quality used in the present study was likely insufficient to overcome other stressors involved in the process of freezing-warming blastocysts, including lipid peroxidation, disruption of the plasma membrane and cell death (Korhonen et al 2012). Currently, several studies (Vajta et al 1999;Fuchinoue et al 2004;Kuwayama et al 2005) have developed improved protocols of mammalian embryo cryopreservation.…”

Section: Discussionmentioning

confidence: 90%

Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants

Rocha-Frigoni

Leão

Nogueira

et al. 2014

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

The effects of intracellular (cysteine and β-mercaptoethanol) and extracellular (catalase) antioxidant supplementation at different times during in vitro production (IVM and/or in vitro culture (IVC)) on bovine embryo development, intracellular reactive oxygen species (ROS) levels, apoptosis and re-expansion rates after a vitrification-thawing process were examined. Blastocyst frequencies were not affected by either antioxidant supplementation (40.5%-56.4%) or the timing of supplementation (41.7%-55.4%) compared with control (48.7%; P>0.05). Similarly, antioxidants and the moment of supplementation did not affect (P>0.05) the total number of blastomeres (86.2-90.5 and 84.4-90.5, respectively) compared with control (85.7). However, the percentage of apoptotic cells was reduced (P<0.05) in groups supplemented during IVM (1.7%), IVC (2.0%) or both (1.8%) compared with control (4.3%). Intracellular ROS levels measured in Day 7 blastocysts were reduced (P<0.05) in all groups (0.60-0.78), with the exception of the group supplemented with β-mercaptoethanol during IVC (0.88), which did not differ (P>0.05) from that in the control group (1.00). Re-expansion rates were not affected (P>0.05) by the treatments (50.0%-93.0%). In conclusion, antioxidant supplementation during IVM and/or IVC reduces intracellular ROS and the rate of apoptosis; however, supplementation does not increase embryonic development and survival after vitrification.

show abstract

Supplementing culture and vitrification-warming media with l-ascorbic acid enhances survival rates and redox status of IVP porcine blastocysts via induction of GPX1 and SOD1 expression

et al. 2014

View full text Add to dashboard Cite

The effect of ascorbic acid during biopsy and cryopreservation on viability of bovine embryos produced in vivo

Cited by 6 publications

References 9 publications

Effect of addition of ascorbate, dithiothreitol or a caspase‐3 inhibitor to cryopreservation medium on post‐thaw survival of bovine embryos produced in vitro

Effect of addition of ascorbate, dithiothreitol or a caspase‐3 inhibitor to cryopreservation medium on post‐thaw survival of bovine embryos produced in vitro

Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants

Supplementing culture and vitrification-warming media with l-ascorbic acid enhances survival rates and redox status of IVP porcine blastocysts via induction of GPX1 and SOD1 expression

Contact Info

Product

Resources

About