The Effect of Ascorbic Acid Oxidation on the Incorporation of Sulfate by Slices of Calf Costal Cartilage

Abstract

A marked inhibition of the incorporation of S35-sulfate by normal calf costal cartilage was produced by potassium ascorbate in the presence of catalytic amounts of cupric ions. The effect of the various components of the ascorbic acid oxidizing system (potassium ascorbate, cupric ions, cuprous ions, hydrogen peroxide, dehydroascorbie acid) was investigated. The results of experiments in which hydrogen peroxide, catalase, or sodium azide were used singly or in combination suggest that the inhibition produced by the aseorbic acid oxidizing system is due, to a considerable extent, to the production of hydrogen peroxide. Dehydroaseorbie acid was also found to inhibit the incorporation of S35-sul/ate by cartilage slices. However, the gradual fall in pH which resulted from the addition of dehydroaseorbic acid could account to a large extent for the inhibitory effect observed because the incorporation of S35-sulfate by cartilage slices decreases sharply as the pH is lowered. The incorporation of SSS-sulfate by cartilage slices is inhibited also by increasing the concentration of phosphate.The state of ascorbic acid deficiency is associated with a defect in the production of intracellular ground substance. The component of ground substance primarily affected, and the mechanisms involved, have not, as yet, been clearly elucidated. A decrease in the incorporation, of S35-sulfate into granulation tissue (1-3), cartilage (4), and a number of other tissues (5) has been observed in scorbutic guinea pigs. Earlier work (6, 7) has established that most of the S36-sulfate retained by an animal beyond 24 hours is found in sulfated mucopolysaccharides. Bostr6m and M~.nsson (8) have studied the incorporation of S36-sulfate into the chondroitin sulfate of costal cartilage slices prepared from normal calves and have indicated a number of factors which influence this reaction. The influence of ascorbic acid on the incorporation of SSS-sulfate by slices of costal cartilage from normal calves is considered in the present communication.* Arthritis and Rheumatism Foundation Fellow.

Autoradiographic Studies with 35S‐Sulfate on Somatotrophin and Estrogen Sensitivite Growth Zones in Rat and Mouse Costal Cartilage

“…I n spite of the frequent utilization of costal cartilage from different animal species in experimental metabolic research, the literature contains only sporadic data about differences in the sulfation pattern between different parts of rib cartilage (Klebanoff et al 1958, Yde 1968). I n most of the studies which have been performed by techniques using isotope incorporation into costal cartilage, the anatomical regional origin of the cartilage samples is not exactly defined.…”

“…To avoid mixing of more or less active cartilage pieces Klebanoff (1958) and Yde (1968) selected the samples by discarding the lateral part of the rib cartilage. By daing this they have eliminated the metabolically most active part of the tissue.…”

Nano/Mesoporous Carbon from Rice Starch for Voltammetric Detection of Ascorbic Acid

“…In this study, we describe a sustainable and cost-effective one-pot synthesis of rice starch-derived nano/mesoporous carbon material (RSNMC) and its application as an electrode material for the detection of ascorbic acid (AA). Ascorbic acid (vitamin C) is an essential nutrient for living cells and plays an important role in metabolic and cell development processes [ 10 , 11 , 12 , 13 ]. It helps develop cells and heal injuries, ulcers, and burns, and is involved in the synthesis of collagen, blood vessels, cartilage, bones, and tendons [ 10 , 11 , 12 , 13 ].…”

“…Ascorbic acid (vitamin C) is an essential nutrient for living cells and plays an important role in metabolic and cell development processes [ 10 , 11 , 12 , 13 ]. It helps develop cells and heal injuries, ulcers, and burns, and is involved in the synthesis of collagen, blood vessels, cartilage, bones, and tendons [ 10 , 11 , 12 , 13 ]. It is also an active component in a variety of pharmaceutical dosage forms such as high potency multivitamin supplements for the prevention and treatment of vitamin C deficiency, common colds, mental illnesses, infertility, cancer and AIDS [ 10 , 11 , 12 , 13 ].…”

Background-free in-vivo Imaging of Vitamin C using Time-gateable Responsive Probe

“…Ascorbic acid (vitamin C), an essential nutrient for humans, has been well known for a long time because of its essential role in the prevention of deficiency diseases, for example, scurvy 1 . It helps also in healthy cell development, calcium absorption, the healing of injuries and burns, and the synthesis of collagen, blood vessels, cartilage, bone and tendons 2 3 4 . In recent years vitamin C has attracted considerable attentions for its role in the promotion of healthy brain aging and potential in cancer treatment 5 6 .…”