The effect of artichoke (<i>Cynara scolymus</i> L.) extract on ROS generation in HUVEC cells

Juzyszyn, Zygmunt; Czerny, Bogusław; Pawlik, Andrzej; Droździk, Marek

doi:10.1002/ptr.2385

Cited by 26 publications

(19 citation statements)

References 9 publications

(8 reference statements)

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“…Some researchers have isolated and identified several bioactive components of artichoke, i.e., caffeic acid, chlorogenic acid, cynarin and luteolin. These bioactive components decrease the production of reactive oxygen species (ROS), lipid peroxidation and the oxidation of low-density lipoproteins in vitro experiments (Juzyszyn et al, 2008;Zapolska-Downar et al, 2002). On the other hand, several studies have reported that vitamin C has some protective activity against Pb intoxication (Patra Shalan et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat

Heidarian

Rafieian-Kopaei

2013

Pharmaceutical Biology

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Context: Artichoke, Cynara scolymus L. (Asteraceae), has many natural antioxidants and multiple pharmacological actions. Recent studies have shown that it has antitoxic activity. Objective: Lead (Pb) is a dangerous environmental toxicant that induces a broad range of dysfunctions in human. This study evaluated the protective effect of the hydroethanolic extract of artichoke against altered biochemical parameters in rats fed with lead-containing diet. Materials and methods: Thirty-two rats were randomly divided into four groups. The first (control) group received standard diet. The second, third and fourth groups received 500 mg lead/kg diet, 500 mg lead/kg diet plus 300 mg/kg b.w. artichoke extract daily, and 500 mg lead/ kg diet plus 1 mg vitamin C/100 g b.w. daily for 6 weeks, respectively. Serum lead, lipoprotein profile, ALT (alanine transaminase), AST (aspartate transaminase), ALP (alkaline phosphatase), malondialdehyde (MDA) and liver histopathology assessments were conducted. Results: Serum lead, triglyceride (TG), VLDL, ALT, AST, ALP and MDA levels decreased significantly (p50.05) in the artichoke-treated group (35.85, 38.26, 38.38, 21.90, 12.81, 26.86 and 46.91%, respectively) compared to lead-intoxicated rats without treatment. No significant change was observed in serum lead, ALP and ALT between artichoke and vitamin C-treated groups (p40.05). Furthermore, the liver histopathology in rats treated with artichoke showed a mild degree of lymphocyte infiltration that was relatively comparable to the control and vitamin C-treated groups. Discussion and conclusion: These results clearly show that the artichoke extract in lead-poisoned rats has suitable chelating properties for the reduction of blood lead levels.

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Section: Introductionmentioning

confidence: 99%

Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat

Heidarian

Rafieian-Kopaei

2013

Pharmaceutical Biology

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“…Artichoke leaf extract (ALE) is also reported to have a cholesterol-reducing effect in hypercholesterolemic subjects [21,22]. In addition, ALE has been found to decrease the production of reactive oxygen species, the oxidation of low density lipoproteins (LDL), and lipid peroxidation in in vitro experiments [23][24][25][26][27]. However, there is no study investigating the effect of ALE on serum lipid levels and tissue pro-oxidant and antioxidant status in experimental hypercholesterolemic conditions.…”

mentioning

confidence: 96%

Effect of Artichoke Leaf Extract on Hepatic and Cardiac Oxidative Stress in Rats Fed on High Cholesterol Diet

Küçükgergin

Aydın

Özdemirler-Erata

et al. 2009

Biol Trace Elem Res

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Hypercholesterolemia and lipid peroxidation play complementary roles in atherosclerosis. Artichoke (Cynara scolymus L., Asteraceae) leaf extract (ALE), rich in antioxidants, has cholesterol-reducing effect. We investigated the effect of ALE on serum and hepatic lipid levels and pro-oxidant-antioxidant balance in the liver and heart of hypercholesterolemic rats. Rats were fed on 4% (w/w) cholesterol and 1% cholic acid (w/w) supplemented diet for 1 month. ALE (1.5 g/kg/day) was given by gavage during the last 2 weeks. High cholesterol (HC) diet caused significant increases in serum and liver cholesterol and triglyceride levels. It increased malondialdehyde (MDA) and diene conjugate (DC) levels in both tissues. Hepatic vitamin E levels and hepatic and cardiac glutathione peroxidase (GSH-Px) activities decreased, but superoxide dismutase and glutathione transferase activities, glutathione, and vitamin C levels remained unchanged due to HC diet. Serum cholesterol and triglyceride levels and ratio of cholesterol to high-density lipoprotein (HDL)-cholesterol decreased in ALE plus HC-treated rats, but liver cholesterol and triglyceride levels remained unchanged. Significant decreases in hepatic and cardiac MDA and DC levels and increases in hepatic vitamin E and GSH-Px activities were observed in ALE-treated hypercholesterolemic rats. Our results indicate that ALE decreases serum lipids and hypercholesterolemia-induced pro-oxidant state in both tissues.

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“…These mechanisms contribute to endothelial dysfunction and may evoke endothelial cell-associated diseases. A number of studies have attempted to identify methods to protect against the inflammatory response and endothelial cell death (9)(10)(11). In the current study, the role of arazyme in the LPS-mediated inflammatory response in HUVECs was investigated.…”

Section: Introductionmentioning

confidence: 99%

Effect of arazyme on the lipopolysaccharide-induced inflammatory response in human endothelial cells

Kim

Yang

Shin

et al. 2014

Molecular Medicine Reports

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Abstract. Arazyme is a novel extracellular metalloprotease secreted by Aranicola proteolyticus. Endothelial cells are involved in the pathogenesis of a number of inflammatory diseases, induce uncontrolled cell viability and express various inflammatory mediators, including cytokines, chemokines, adhesion molecules and reactive oxygen species (ROS). In the current study, human umbilical vein endothelilal cells (HUVECs) were used to investigate the anti-inflammatory effects of arazyme following lipopolysaccharide (LPS) stimulation. Apoptosis of HUVECs due to LPS was inhibited by arazyme. In various inflammatory responses induced by LPS, arazyme inhibited the secretion of the monocyte chemoattractant protein-1 and interleukin-6, and the expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1. Arazyme also suppressed ROS production in HUVECs. The action of arazyme was not associated with NF-κB activity in HUVECs. These results indicate that arazyme has anti-inflammatory properties in inflamed endothelial cells and may be useful as a therapeutic agent for inflammatory diseases associated with endothelial cells.

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The effect of artichoke (Cynara scolymus L.) extract on ROS generation in HUVEC cells

Cited by 26 publications

References 9 publications

Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat

Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat

Effect of Artichoke Leaf Extract on Hepatic and Cardiac Oxidative Stress in Rats Fed on High Cholesterol Diet

Effect of arazyme on the lipopolysaccharide-induced inflammatory response in human endothelial cells

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