The effect of applications of eCG and PGF2? at different times with FGA containing vaginal sponges on reproductive parameters in ewes at anestrous season

Abstract

ÖzAmaç: Çalışmada FGA içeren süngerlerle östrüsleri uyarılan anöstrüsteki İvesi koyunlarında, eCG ve PGF2α enjeksiyonlarının süngerlerin çıkarıldığı anda veya 48 saat öncesinde uygulanmasının fertilite üzerindeki etkilerinin incelenmesi amaçlandı. Gereç ve Yöntem:Çalışma 80 İvesi koyun üzerinde yürütül-dü. Süngerler vaginada 12 gün tutuldu. Grup 1'deki (n=40) koyunlara süngerlerin çıkarıldığı anda, Grup 2'deki (n=40) koyunlara ise süngerlerin çıkarılmasından 48 saat önce 400 IU eCG ve 125 mcg kloprostenol kas içi yolla enjekte edildi. Ayrıca her iki gruptaki koyunlara süngerlerin çıkarılmasın-dan 40 saat sonra 5 mcg buserelin asetat kas içi yolla uygulandı.Bulgular: Östrüs başlangıç zamanı Grup 1 ve 2'de sırasıyla 35.08±1.75 ve 32.12±1.41 saat olarak saptandı. Östrüs, gebelik ve konsepsiyon oranları Grup 1'de sırasıyla %68.4, %55.2, %80.7 olarak hesaplanırken; Grup 2'de %89.4, %76, %85.2 olarak belirlendi. Kuzulama oranları Grup 1 ve 2'de sırasıy-la %95.2 ve %100 olarak belirlendi. İkizlik oranı ve kuzu verimleri sırasıyla Grup 1'de %50 ve %150 iken, Grup 2'de %31 ve %131 olarak tespit edildi. Grup 2'nin östrüs oranı Grup 1'den istatistik olarak yüksek (P<0.05) bulunmasına rağmen Gruplar arasında diğer fertilite parametrelerinde fark tespit edilmedi (P>0.05).Öneri: Koyunlarda süngerlerin çıkarıldığı anda eCG ve kloprostenolün enjekte edilmesiyle işgücü yönünden avantaj sağ-layacağı ifade edilebilir. Anahtar kelimeler: Koyun, anöstrüs, FGA, eCG, kloprostenol AbstractAim: The aim of this study was to compare the fertility obtained with eCG and PGF2α treatment, at time of FGA impregnated sponge withdrawal or 48 h before, in ewes during anoestrous season. Materials and Methods:Research was conducted on 80 ewes. The FGA containing sponges were maintained in the vagina for 12 days. Ewes received an intramuscular injection of 400 IU eCG and 125 mcg cloprostenol at the time of sponge withdrawal in the Group1 (n=40) and 48 hours before sponge removal in the Group 2 (n=40). Additionally, all of the ewes in the study received 5 mcg buserelin acetate intramuscularly 40 hours after the sponge withdrawal.Results: Intervals from sponge removal and the onset of estrus were 35.08±1.75 and 32.12±1.41 hours in Group 1 and Group 2, respectively. The estrus, pregnancy and conception rates were 68. 4%, 55.2%, 80.7% and 89.4%, 76%, 85.2% in the Groups 1 and 2, respectively. The twinning rate and litter sizes were 50% and 31%, 150% and 131% in the Groups 1 and 2, respectively. Estrus rate of Group 2 was statistically higher than Group 1 (P<0.05). No statistical difference was found in other fertility parameters between the groups (P>0.05). Conclusion:It may be stated that the injections of eCG and cloprostenol at the time of sponge removal is supposed an advantage for less requirement of visit to the farms.

Post‐mating diclofenac vs. carprofen treatment on serum progesterone levels and reproductive outcomes in Hungarian‐Merino ewes during the non‐breeding season

“…Similarly, in experiment 2, there was no difference in pregnancy rates as a result of post‐mating carprofen treatment ( p > .05) (Table 1). Although an increase in pregnancy rates was observed with post‐mating double‐dose diclofenac application in goats (Aksu, 2019; Doğruer et al, 2012), no increase was observed in pregnancy rates in our study in ewes. Previous studies of carprofen treatment in dairy cows have reported varying degrees of positive responses differing from 12% (Özkök, 2019) to 17% (Alkan et al, 2021) in pregnancy rate.…”

“…Conversely, Allabban and Erdem (2020) reported that administration of FM (double dose days 9 and 10 after mating) increased the conception rate by 7.6% in Awassi ewes during the breeding season. Furthermore, in Damascus goats, it has been reported that administration of double‐dose diclofenac sodium on post‐mating day 14–16 increases the pregnancy rates by 25.9% (Doğruer et al, 2012) and 20.8% (Aksu, 2019).…”

“…In farm animals, the use of NSAIDs in inhibiting luteolytic PGF 2α secretion as a result of insufficient IFN‐τ secretion is a commonly used strategy in management of embryonic losses in early pregnancy (Binelli et al, 2001; Erdem & Guzeloglu, 2010). It has been reported that NSAIDs can be injected after mating to prevent embryonic losses and increase fertility (Doğruer et al, 2012). In cattle, the efficacy of several NSAIDs including flunixin meglumine, carprofen, meloxicam, ibuprofen and aspirin has been investigated to increase pregnancy rates and prevent embryonic deaths (Damarany & Ghanem, 2020; Jaskowski et al, 2021).…”

“…In farm animals, the use of NSAIDs in inhibiting luteolytic PGF 2α secretion as a result of insufficient IFNτ secretion is a commonly used strategy in management of embryonic losses in early pregnancy (Binelli et al, 2001;Erdem & Guzeloglu, 2010). It has been reported that NSAIDs can be injected after mating to prevent embryonic losses and increase fertility (Doğruer et al, 2012).…”

Oestrus synchronisation with progesterone-containing sponge and equine chorionic gonadotropin in Pirlak ewes during the non-breeding season: can Toryum improve fertility parameters?

“…After synchronisation with progestogens during the non-breeding season, the onset of oestrus occurred after 22.5–35.1 h in Kıvırcık ewes ( 6 ) and 32.1–35.1 h in Awassi ewes ( 7 ). Segureña ewes were synchronised with controlled internal drug release (CIDR) and eCG treatment for 5, 6, 7, and 14 days.…”