The Effect of Antiretroviral Naïve HIV-1 Infection on the Ability of Natural Killer Cells to Produce IFN-&amp;#x03B3; upon Exposure to Plasmodium falciparum-Infected Erythrocytes

Sake, Carole Stéphanie; Ngu, Loveline N.; Ambada, Georgia; Chedjou, Jean Chamberlain; Nji, Nadesh; Tchadji, Jules Colince; Lissom, Abel; Tchouangueu, Thibau Flaurant; Djukouo, Larissa; Njambe, Ghislain; Garcia, Rosario; Gutiérrez, Anna; Waffo, Alain Bopda; Park, Chae Gyu; Mbacham, Wilfried; Etoa, François–Xavier; Nchinda, Godwin

doi:10.1159/000467386

Cited by 3 publications

(3 citation statements)

References 27 publications

(34 reference statements)

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“…HIV-1 positive participants were members of the CIRCB AFRODEC cohort established since 2012. The CIRCB AFRODEC (African HIV-1 dendritic cell targeted vaccine) cohort study was established in Cameroon in 2012 and enrolled antiretroviral therapy (ARV) naïve HIV-1 infected people [29][30][31]. HIV negative Participants were confirmed for their negative status using rapid tests including Determine™ HIV1/2 test kit (Alere™) and KHB Shanghai HIV 1+2 diagnostic test kits (Kehua Bio-engineering Co., Ltd. China).…”

Section: Methodsmentioning

confidence: 99%

“…This study was carried out in three site including; the vaccinology laboratory of the Chantal BIYA international reference Centre (CIRCB), the Catholic Health Centre of Bikop (at Bikop) and Catholic Health Centre of Mvog-Beti (Yaounde) where blood sample were collected from consented participants. All participants were members of the CIRCB AFRODEC cohort [29][30][31]. Participants were constituted into four groups consisting of dual HIV-malaria infected (HIV+/ Mal+), HIV mono-infected (HIV+/Mal-), malaria mono-infected (HIV-/Mal+) and double negative (HIV-/Mal-) individuals.…”

Section: Study Populationmentioning

confidence: 99%

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Comparative Analysis of Igg Responses to Recombinant Qβ Phage Displayed MSP3 and UB05 in Dual HIV-Malaria Infected Adults Living in areas differing in Malaria Transmission Intensities

2019

JCEI

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Immunoglobulin G (IgG) specific responses against Plasmodium falciparum merozoite antigens such as the merozoite surface protein 3 (MSP3) and UB05 are known to play critical roles in parasiteamia control and protection from symptomatic illness. However when there is intense perennial malaria transmission coupled with concurrent infection with the human immunodeficiency virus type 1 (HIV), knowledge of IgG antibody response profiles is limited. In this study we assessed the impact of dual HIV-Malaria infections on IgG subclass responses to MSP3 (QβMSP3) and UB05 (QβUB05) in individuals living in two areas of Cameroon differing in malaria transmission intensity. We observed differences in antigen specific IgG and IgG subclass responses which were dependent upon the antigen type, malaria transmission intensity, HIV infection, malaria infection and dual HIV-malaria infections. Individuals living in high malaria transmission areas irrespective of HIV or malaria status had significantly higher IgG responses to both antigens (P=0.0001 for QβMSP3, P=0.0001 for QβUB05) than their counterpart from low transmission areas. When dual HIV-Malaria infection is considered significantly higher QβMSP3 specific IgG1 (P=0.0001) and IgG3 (P=0.04) responses in double negative individuals was associated with protection against malaria in low transmission areas. Increased QβUB05 specific IgG1 responses (P=0.0001) in double negative individuals were associated with protection in high transmission areas in contrast to significantly higher IgG3 responses to QβUB05 (P=0.0001) which were more relevant to protection in low malaria transmission areas in the same population. These findings imply that QβMSP3 might not be suitable as a standalone vaccine in areas differing in transmission intensity. However, antigenicity of UB05 most likely predicts immunity in both low and high transmission areas and could be used either alone or in combination with other antigens for vaccine studies in areas differing in transmission intensities. Understanding immune responses to QβUB05 and QβMSP3 could thus enable the development of efficacious vaccines or commensurate immunotherapeutic strategies suitable for areas differing in malaria transmission intensity.

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Section: Methodsmentioning

confidence: 99%

Section: Study Populationmentioning

confidence: 99%

Comparative Analysis of Igg Responses to Recombinant Qβ Phage Displayed MSP3 and UB05 in Dual HIV-Malaria Infected Adults Living in areas differing in Malaria Transmission Intensities

2019

JCEI

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“…Participants with other infection (including microfilaria, dengue, TB, and hepatitis B and C) and pregnant women were excluded from the study. All participants were members of the CIRCB AFRODEC cohort (28–30)…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of IgG Responses to recombinant Qβ phage displayed MSP3 and UB05 in Dual HIV-malaria infected adults living in areas differing in Malaria transmission intensities

Lissom

Ouambo

Megnekou

et al. 2018

Preprint

Self Cite

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Immunoglobulin G specific responses againstPlasmodium falciparummerozoite antigens such as the merozoite surface protein 3 (MSP3) and UB05 are known to play critical roles in parasitemia control and protection from symptomatic illness. However when there is intense perennial malaria transmission coupled with concurrent infection with the human immunodeficiency virus type 1 (HIV), knowledge of IgG antibody response profiles is limited. In this study we assessed the impact of dual HIV-Malaria infections on IgG subclass responses to MSP3 (QβMSP3) and UB05 (QβUB05) in individuals living in two areas of Cameroon differing in transmission intensity. We observed differences in antigen specific IgG and IgG subclass responses which was dependent upon the antigen type, malaria transmission intensity, HIV infection, malaria infection and dual HIV-malaria infections. Individuals living in high malaria transmission areas irrespective of HIV or malaria status had significantly higher IgG responses to both antigens (P=0.0001 for QβMSP3, P=0.0001 for QβUB05) than their counterpart from low transmission areas. When dual HIV-Malaria infection is considered significantly higher QβMSP3 specific IgG1 (P=0.0001) and IgG3 (P=0.04) responses in double negative individuals was associated with protection against malaria in low transmission areas. Superior QβUBO5 specific IgG1 responses (P=0.0001) in double negative individuals were associated with protection in high transmission areas in contrast to significantly higher IgG3 responses to QβUB05 (P=0.0001) which were more relevant to protection in low malaria transmission areas in the same population. Thus, understanding immune responses to QβUB05 and QβMSP3 could facilitate the development of immunotherapeutic strategies suitable for areas differing in malaria transmission intensity.

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Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing

Ntemafack

Dzelamonyuy

Nchinda

et al. 2023

Viruses

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We selected a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nucleotides. From a 15-mer library displayed on the RNA coliphage Qβ, a 15-amino acid long peptide (HGHGWQIPVWPWGQG) hereby referred to as a nanotag was identified to selectively bind biotin. The target selection was achieved through panning with elution by infection. The selected peptide was tested as a transducer for an immunogenic epitope of the foot-and-mouth disease virus (FMDV) on Qβ phage platform separated by a linker. The biotin-tag showed no significant influence on the affinity of the epitope to its cognate antibody (SD6). The nanotag-bound biotin selectively fused either to the C- or N-terminus of the epitope. The epitope would not bind or recognize SD6 while positioned at the N-terminus of the nanotag. Additionally, the biotin competed linearly with the SD6 antibody in a competitive ELISA. Competition assays using the selected recombinant phage itself as a probe or transducer enable the operationalization of this technology as a biosensor toolkit to sense and quantify SD6 analyte. Herein, the published Strep II nanotag (DVEWLDERVPLVET) was used as a control and has similar functionalities to our proposed novel biotin-tag thereby providing a new platform for developing devices for diagnostic purposes.

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The Effect of Antiretroviral Naïve HIV-1 Infection on the Ability of Natural Killer Cells to Produce IFN-γ upon Exposure to Plasmodium falciparum-Infected Erythrocytes

Abstract: Background: In sub-Saharan Africa, intense perennial Plasmodium species transmission coincides with areas of high prevalence of the human immunodeficiency virus type 1 (HIV) infection. This implies that antiretroviral naïve HIV-infected people living within these regions

Cited by 3 publications

References 27 publications

Comparative Analysis of Igg Responses to Recombinant Qβ Phage Displayed MSP3 and UB05 in Dual HIV-Malaria Infected Adults Living in areas differing in Malaria Transmission Intensities

Comparative Analysis of Igg Responses to Recombinant Qβ Phage Displayed MSP3 and UB05 in Dual HIV-Malaria Infected Adults Living in areas differing in Malaria Transmission Intensities

Comparative analysis of IgG Responses to recombinant Qβ phage displayed MSP3 and UB05 in Dual HIV-malaria infected adults living in areas differing in Malaria transmission intensities

Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing

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