The Effect of Antioxidants on Micropropagation of Avocado by Nodal Segments

Güler, Gizem; Gübbük, Hamide; Arslan, Mahmut Alper

doi:10.16882/hortis.900936

Cited by 1 publication

(2 citation statements)

References 18 publications

(19 reference statements)

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“…Vásquez-Hernández et al (2021) obtuvieron 43 % de explantes viables de Pimienta dioica utilizando concentraciones de 50 -200 mg L -1 de cisteína agregada en medio de cultivo MS. Al esterilizar el medio de cultivo los componentes de este se degradan, existiendo cambios en su composición química (García-González et al, 2010), probablemente el efecto de la cisteína en los explantes de aguacate fue debido a estas reacciones en el medio de cultivo. Para aguacate además del uso de cisteína, también se ha experimentado con antioxidantes como: ácido ascórbico, ácido cítrico, carbón activado y polivinilpirrolidona (PVP), obteniendo hasta 83 % de explantes viables (Güler et al, 2021).…”

Section: Uso De L-cisteína Como Antioxidanteunclassified

“…Otro problema, es la oxidación que causa muerte de los explantes. Para solucionarlo se han utilizado antioxidantes aplicándolos al medio de cultivo o exponiendo el material vegetal por minutos en solución acuosa (Güler et al, 2021). Para poder multiplicar aguacate in vitro es importante desarrollar un protocolo eficiente para la desinfección de explantes y evitar oxidación, además que los productos utilizados no sean tóxicos para explantes, usuario y ambiente.…”

Section: Introductionunclassified

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Productos Alternativos en La Desinfestación in Vitro en Explantes De Portainjerto Duke-7 E Identificación De Microorganismos Endófitos

Castro Garibay,

Monter,

Hernández Nava

et al. 2023

Trop Subtrop Agroecosyst

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Background. The vegetative material for in vitro establishment of avocado explants is from the field, for this reason, the proportion of contamination, oxidation and mortality of explants is elevated, therefore we are looking for alternatives to decreased. Objective. To evaluate concentrations of alternative products for disinfestation in avocado explants cv Duke 7, and decrease oxidation with the addition of L-cysteine in the in vitro establishment. Methodology. The products used were: peroxyacetic acid (AP), chlorine dioxide (DC), and quaternary ammonium salts (ACS), in three concentrations. 54 explants were used in each treatment, and placed in culture medium for establishment, evaluating contamination (%) and oxidation (%). Two weeks later, 50% of viable explants from each treatment were immersed in L-cysteine (100 mgL-1) and placed in a culture medium for multiplication, the rest was placed a in multiplication medium containing L-cysteine; only oxidation was evaluated (%). Contaminated explants were isolated, identified, and characterized fungi and bacteria. Results. The best results of the disinfestation were with ACS with 3.7% contamination, without oxidation. Regarding the use of L-cysteine (100 mgL-1), the lowest percentage of oxidation (47%) was obtained when immersed in a cysteine solution. Three genera of fungi were identified: Aspergillus (3), Penicillium (1) and Cladosporium (1), and Agrobacterium as a bacterium. Implications. With the proposed methodology, we can continue with the following stages of in vitro culture, to establish methodologies for budding, growth, and rooting of avocado clonal rootstocks. Conclusions. The use of ACS and cysteine in solution reduces both contamination and oxidation of explants, but it is necessary to modify concentrations and exposure times in the disinfestation protocol, in addition to identifying even the genus the microorganisms found.

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Section: Uso De L-cisteína Como Antioxidanteunclassified

Section: Introductionunclassified