The Effect of Antioxidant Vitamins Ε and C on Lipoperoxidation of Erythrocyte Membranes During Hyperbaric Oxygenation

Etlik, Özdal; Tomur, Ahmet; Dündar, Kadir; Erdem, Abdullah; Gündoğan, Nimet Ünay

doi:10.1515/jbcpp.1997.8.4.269

Cited by 19 publications

(9 citation statements)

References 17 publications

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“…The correlation between osmotic fragility and oxidative stress (produced in diabetes by the reactive oxygen-derived free radicals) relies on peroxidation of cholesterol and linoleic acid molecules of erythrocyte plasmalemma (Inouye et al 1999a(Inouye et al , 1999b(Inouye et al , 1999c. Moreover, antioxidants were reported to decrease the osmotic fragility of erythrocytes (Etlik et al 1997;Kraus et al 1997). In the hamster experimental model of simultaneous hyperlipemia-hyperglycemia, L-arginine administration resulted in a decrease in the osmotic fragility of erythrocyte plasmalemma, a consequence of decline in lipid peroxidation (Lubec et al 1997;Ozcelikay et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Beneficial effects of L -arginine supplementation in experimental hyperlipemia-hyperglycemia in the hamster

Popov¹,

Costache²,

Georgescu³

et al. 2002

Cell and Tissue Research

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The objective of this study was to evaluate whether administration of L-arginine, the substrate for nitric oxide synthesis, was able to ameliorate the endothelial dysfunction and the morphological changes induced by the combined insult of hyperlipemia and hyperglycemia. To this purpose, golden Syrian hamsters were rendered simultaneously hyperlipemic and diabetic (HD group) for 24 weeks, and then orally treated with 622.14 mg/kg per day L-arginine, for 12 weeks (HD + L-arg group). The following assays were carried out: (1) spectrophotometric: concentrations of circulating glucose, cholesterol, and creatinine, the activity of angiotensin-converting enzyme (ACE), and the osmotic fragility of erythrocyte plasmalemma; (2) myographic: the endothelium-dependent and -independent relaxation of the resistance arteries (i.d. 210-250 microm) to 10(-8) to 10(-4) M acetylcholine (ACh) or sodium nitroprusside (SNP); and (3) electron-microscopic: the ultrastructure of the resistance arteries, myocardium, and kidney glomeruli, which are main targets of hypertensive complications. The results showed that oral supplementation with L-arginine in simultaneous hyperlipemia-hyperglycemia induced in hamsters had favorable effects on: (1) homeostasis, i.e., diminished the concentration of circulating glucose (by ~63%) and cholesterol (by approximately 10%), reduced the ACE activity (by approximately 45%), and lowered the osmotic fragility of erythrocyte plasmalemma (as marker for the oxidative stress in plasma); (2) mesenteric resistance arteries, which showed (in 10(-4) M ACh) an improved endothelium-dependent relaxation (72.40+/-4.6% in the HD + L-arg group vs 61.90+/-1.45% in the HD group) and a reduced thickness (approximately 1.32-fold) of the smooth muscle cells' extracellular matrix; and (3) the heart, which displayed approximately 16% diminishing of the thickness of the left ventricular wall, and an apparently normal structure of the myocardium; the restoration of the thickness of the pericapillary extracellular matrix to almost normal dimensions was also observed. Administration of L-arginine did not modify the high level of plasma creatinine determined for the HD group (approximately 48% increased vs control group) and had no effect on the thickened, nodular basal lamina of the kidney capillaries. The results indicate that endothelial dysfunction established in combined hyperlipemia-diabetes is distinctive for each vascular bed (mesenteric arterioles, heart capillaries, kidney glomerular capillaries), and there is a reversible stage of the dysfunction in which L-arginine oral supplementation induced beneficial effects.

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Section: Discussionmentioning

confidence: 99%

Beneficial effects of L -arginine supplementation in experimental hyperlipemia-hyperglycemia in the hamster

Popov¹,

Costache²,

Georgescu³

et al. 2002

Cell and Tissue Research

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“…The inhalation of oxygen in greater than typical concentrations can stimulate ROS generation in several cell types; however, the results of studies are conflicting. Some studies of HBO treatments show that oxidative damage in several cell types increases, [12][13][14][15] while others conclude the opposite. [16][17][18][19] The effect of HBO treatment on renal function in sepsis is unknown.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Hyperbaric Oxygen Treatment on the Renal Functions in Septic Rats: Relation to Oxidative Damage

et al. 2005

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“…In summary, a sufficient level of oxygen is required for the killing effects of PMNs and macrophages to emerge, and in addition the production of O 2 and H 2 O 2 is parallel to the tissue oxygen tension (Park, 2002). It has also been reported that toxic oxygen species may also occur from other cells such as erythrocytes after the application of HBO (Etlik et al, 1997).…”

Section: Discussionmentioning

confidence: 98%

The effect of hyperbaric oxygenation on thein vitro growth ofEscherichia coli in environments with and without blood cells

et al. 2006

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The aim of this study was to investigate whether the in vitro presence of blood cells influences the anti-microbial activity of hyperbaric oxygen (HBO) against Escherichia coli. Fifty E. coli isolates from clinical samples were used in the study. A small number of colonies belonging to each isolate from the nutrient media were transferred into two K 3 EDTA tubes (the blood group) and two Mueller-Hinton broth tubes (the broth group). Then, both groups were divided into subgroups according to whether HBO was administered (HBO subgroup) or not (non-HBO subgroup). HBO treatment was applied for one hour at 2.5 absolute atmospheres. The tubes in the non-HBO subgroup were left at room temperature during this period. Subsequently, all the tubes were cultured on Mueller-Hinton and Eosin Methylene Blue agar using the quantitative counting technique. After 18 to 24 h incubation at 37 ºC, the colonies formed in the plates were counted. In the blood group, compared with non-HBO subgroup samples, the number of colonies decreased in 56% of samples, increased in 32% of samples and did not change in 12% of samples in the HBO subgroup. Whereas, in the broth group the number of colonies decreased in only 32% of samples increased in 38% of samples and did not change in 30% of samples in the HBO subgroup compared with the non-HBO subgroup. The difference between the blood and the broth groups revealed a statistical significance using Pearson's Chi-square test (P = 0.025). We concluded that the antibacterial effect of HBO on E. coli increases in the cellular environment belonging to the host organism.

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The Effect of Antioxidant Vitamins Ε and C on Lipoperoxidation of Erythrocyte Membranes During Hyperbaric Oxygenation

Cited by 19 publications

References 17 publications

Beneficial effects of L -arginine supplementation in experimental hyperlipemia-hyperglycemia in the hamster

Beneficial effects of L -arginine supplementation in experimental hyperlipemia-hyperglycemia in the hamster

The Effect of Hyperbaric Oxygen Treatment on the Renal Functions in Septic Rats: Relation to Oxidative Damage

The effect of hyperbaric oxygenation on thein vitro growth ofEscherichia coli in environments with and without blood cells

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