The effect of antioxidant treatment on human spermatozoa and fertilization rate in an in vitro fertilization program

The role of antioxidant therapy in the treatment of male infertility: an overview

“…Only four of these studies [28][29][30][31] found little or no effect on semen parameters, whereas beneficial effects were reported in the remaining 18 studies. 8,19,[32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47] In vitro studies.…”

“…In 1995, Kessopoulou et al 28 and Moilanen and Hovatta 29 administered vitamin E 600 mg day 21 for 3 months and 600, 800 or 1200 mg day 21 for 3 weeks, respectively, finding increased concentrations of vitamin E in plasma and semen but no changes in semen parameters. In 1996, Geva et al 30 conducted a prospective study on 15 antiretroviral therapy patients, in which the administration of 200 mg day 21 for 3 months produced no changes in sperm morphology. Similarly, Rolf et al 31 found no positive effects after the administration of a combination of vitamins C and E (1000 and 800 mg daily) for 56 days, with no increase in motility, viability, concentration and morphology in any of the 33 asthenozoospermic patients who participated in the study.…”

“…Kessopoulou et al, 1995 28 No effects (600 mg day 21 for 3 months, in vivo) Moilanen and Hovatta, 1995 29 No effects (600-1200 mg day 21 for 3 weeks, in vivo) Geva et al, 1996 30 No effects (200 mg day 21 for 3 months, in vivo) Vezina et al, 1996 35 Increased motility, morphology, viability; no effects on concentration (400 mg day 21 1 100-200 mg day 21 Se for 6 months, in vivo) Suleiman et al, 1996 36 Increased pregnancy rate, increased motility, reduced lipid peroxidation (300 mg day 21 for 6 months, in vivo) Kodama et al, 1997 37 Increased concentration, reduced DNA damage (200 mg vitamin C1200 mg vitamin E1GSH 400 mg every day for 2 months, in vivo) Hughes et al, 1998 38 Reduced DNA damage after Percoll preparation (30-60 mmol l 21 vitamin E, in vitro) Hughes et al, 1998 38 Increased DNA damage after Percoll preparation (300-600 mmol l 21 vitamin C1 30-60 mmol l 21 vitamin E, in vitro) Twigg et al, 1998 19 No effects on NADPH-induced lipid peroxidation (1 mmol l 21 , in vitro) Donnelly et al, 1999 39,40 Reduced H 2 O 2 -induced ROS production and DNA damage (40-60 mmol l 21 , in vitro) Rolf et al, 1999 31 No effects (800 mg vitamin E11000 mg vitamin C daily for 56 days, in vivo) Comhaire et al, 2000 41 Reduced ROS quantity; no effects on semen parameters (30 b-carotene1180 mg vitamin E daily for 3 months, in vivo) 38 Increased DNA damage after Percoll preparation (300-600 mmol l 21 vitamin C1 30-60 mmol l 21 vitamin E, in vitro) Verma and Kanwar, 1998 34 Preserved motility (800 mmol l 21 , in vitro) Donnelly et al, 1999 39,40 Reduced H 2 O 2 -induced ROS production and DNA damage (300-600 mmol l 21 , in vitro) Rolf et al, 1999 31 No effects (800 mg day 21 vitamin E11000 mg day 21 To be continued…”

Role of Antioxidants in the Treatment of Male Infertility

“…In a prospective study, 15 normozoospermic men with low fertilization rates in their earlier ART cycles were treated with 200mg/day of vitamin E for three months. The high MDA concentration declined to normal rate and the fertilization rate per cycle significantly improved after one month of treatment [56]. Finally, dietary habits seem also to play a role in semen quality, since a positive correlation has been found between vitamin E dietary intake and progressive and total motility Surg Med Open Acc J Volume 1 -Issue -2 [50].…”

“…The administration resulted in improved sperm motility and ability to bind the hamster oocyte in the hamster egg penetration test [53]. In other studies, lower doses (200 or 300mg/ day) have been used [56].…”