The Effect of Antimicrobial Agents on Leukocyte Chemotaxis

Esterly, Nancy B.; Furey, Nancy; Flanagan, Lillian E.

doi:10.1111/1523-1747.ep12543487

Cited by 244 publications

(80 citation statements)

References 27 publications

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“…Other researchers, however, have reported significant inhibition with the same range of concentrations (8,10,23) or enhancement of neutrophil migration with high concentrations (50 to 500 ,ugIml) of erythromycin (2). It must be noted that in these experiments (2, 8, 10) a Boyden chamber was used for the chemotaxis assay, whereas the under-agarose technique was used here.…”

Section: Discussionmentioning

confidence: 87%

Synergy between RU 28965 (roxithromycin) and human neutrophils for bactericidal activity in vitro

Labro¹,

Amit²,

Babin-Chevaye³

et al. 1986

Antimicrob Agents Chemother

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The in vitro effects of RU 28965 (roxithromycin), a new semisynthetic macrolide, on human neutrophil activity were compared with those of erythromycin. RU 28965, at a concentration as low as 0.1 ,ig/ml, Formnyl-methionyl-leucyl-phenylalanine (FMLP), 10-7 M, and zymosan-activated serum were used as chemoattractants. Antibiotics or DMSO alone, diluted as previously indicated, were used as follows: (i) as direct chemoattractants; (ii) after incubation with PMN for 30 min at 37°C under 5% CO2; and (iii) directly incorporated in agarose as previously described (28).(ii) MPO activity. Activity of the myeloperoxidase (MPO) system of PMN was tested by the iodination ability of PMN (13,29) in the presence of antibiotics, DMSO, or buffer. An iodination test was also performed after incubation of PMN with these reagents for 30 min at 37°C.(iii) Ingestion. Ingestion was tested by two different assays: (i) with killed, radiolabeled K. pneumoniae as previously described (32), using 100 bacteria per PMN in the assay medium; and (ii) by the method used to test bactericidal activity (see below). These two techniques measure PMN-associated bacteria, i.e., ingested plus membraneadherent bacteria.

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Section: Discussionmentioning

confidence: 87%

Synergy between RU 28965 (roxithromycin) and human neutrophils for bactericidal activity in vitro

Labro¹,

Amit²,

Babin-Chevaye³

et al. 1986

Antimicrob Agents Chemother

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“…Clindamycin, erythromycin and tetracycline inhibit chemotaxis of human leukocytes at concentrations below therapeutic blood levels (Esterly, Furey and Flanagan, 1978). There is very little information on the antibiotic concentrations attained in comedonal material although therapeutic concentrations of clindamycin are achieved in some people using topical application of clindamycin (Guin et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Resistance of propionibacteria to antibiotics used in the treatment of acne

Brown

Poston

1983

Journal of Medical Microbiology

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“…In addition, it is hypothesized that this high concentration of EM within the neutrophil cytoplasm may modulate neutrophil functions. In fact, EM-mediated inhibition of neutrophil chemotaxis (9,27,29,31,32), oxidant generation (1,13,27), and phagocytosis (27) have been reported. In addition, EM also shortens neutrophil survival by accelerating apoptosis (2).…”

Section: Methodsmentioning

confidence: 99%

Effect of Macrolide Antibiotics on Macrophage Functions

Fujita

Negayama

et al. 1996

Microbiology and Immunology

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Macrolide antibiotics have a variety of actions other than antimicrobial activities. Recently, it has been suggested that macrolide antibiotics act as immunomodulators.In this study, we evaluated the effects of macrolide antibiotics on macrophage functions.For the macrophage, we used the mouse macrophage cell line J774.1. The following effects of macrolide antibiotics on macrophage functions were evaluated: the effect of macrolide antibiotics on macrophage growth; the phagocytosis of beads; cytocidal activity against Candida albicans; and chemotaxis to lipopolysaccharide (LPS). Macrolide antibiotics except for azithromycin significantly stimulated the growth of the macrophage. In addition, pretreatment with macrolide antibiotics except for roxithromycin significantly stimulated the macrophage phagocytosis of beads, macrophage chemotaxis to LPS, and macrophage cytocidal activity against Candida albicans. These results suggest that macrolide antibiotics stimulate macrophage functions. There is increasing evidence that long-term administration of the macrolide antibiotic erythromycin (EM) is effective in the treatment of chronic respiratory infections, probably through actions other than its antimicrobial properties (19). Although the mechanism of the efficacy is uncertain, several hypotheses have been proposed, such as the immunomodulatory action of macrolide antibiotics. Macrolide antibiotics modulate the functions of inflammatory cells such as polymorphonuclear leukocytes, lymphocytes and macrophages (1, 33). In addition, macrolide antibiotics directly affect airway secretory cell (12) and epithelial cell (35) functions. These effects are proposed to explain the efficacy of macrolides in the treatment of inflammatory airway diseases. Several studies have been conducted concerning the interaction between phagocytic cells and macrolide antibiotics. However, there are few reports that evaluate the interaction between macrophage functions and several macrolide antibiotics. With this background, we evaluated various aspects of the interaction between four macrolide antibiotics and macrophages. These studies included the effect on growth, effect on phagocytic activity, effect on chemotaxis to lipopolysaccharide (LPS), and effect of cytotoxicity against Candida albicans. Materials and MethodsMacrophage. We used a murine macrophage cell line, J774.1. This cell line was obtained from the RIKEN Cell Bank (Ibaraki, Japan). This cell line was cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS).Antibiotics. The EM (Japan Dynabot Co., Tokyo), clarithromycin (CAM, Japan Dynabot Co.), roxithromycin (RXM, Roussel Uclaf, Paris, France), and azithromycin (AZM, Pfizer Pharmaceuticals Inc., Japan) were provided by the respective manufacturers. These 4 drugs were dissolved in 90% methanol at a concentration of 2 mg/ml as a stock solution; then diluted with RPMI-1640 medium with 10% FBS. Several final concentrations of macrolide antibiotics (4 concentrations of each antibiotic) were used in the following experimen...

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The Effect of Antimicrobial Agents on Leukocyte Chemotaxis

Cited by 244 publications

References 27 publications

Synergy between RU 28965 (roxithromycin) and human neutrophils for bactericidal activity in vitro

Synergy between RU 28965 (roxithromycin) and human neutrophils for bactericidal activity in vitro

Resistance of propionibacteria to antibiotics used in the treatment of acne

Effect of Macrolide Antibiotics on Macrophage Functions

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