The effect of anionic surfactant on poliovirus particles during capillary electrophoresis

Oita, Iuliana; Halewyck, Hadewych; Thys, Bert; Rombaut, Bartholomeus; Heyden, Yvan Vander

doi:10.1016/j.jpba.2012.07.033

Cited by 5 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the same manner as for Qβ, the presence of SDS leads to the increase of the absolute electrophoretic mobility of heat‐formed subviral particles of MS2. Interestingly, similar observations were reported for subviral particles of poliovirus also formed by heat treatment , and also characterized by a higher hydrophobicity compared to the native poliovirus . In another study , it was reported closed mobility of two serotypes of human rhinovirus (HRV) in CZE performed with the nonionic surfactant Thesit as BGE additive, suggesting closed surface charge of both HRV serotypes, while only one exhibited an increase of its absolute electrophoretic mobility when Thesit was substituted by SDS.…”

Section: Resultssupporting

confidence: 84%

“…In papers concerning poliovirus, reproducible electropherograms were obtained with untreated capillary even without any BGE additives by using highly purified viral samples . Efficient CZE analyses were also reported by Liang et al .…”

Section: Introductionmentioning

confidence: 73%

“…The use of the surfactant SDS as BGE additive allows to overcome the common troubles of irreproducibility encountered in the CZE analyses of non‐enveloped icosahedral viruses . Although BGE additives may not be necessary in some cases , when the presence of SDS is required, it becomes problematic to understand the behavior of non‐enveloped icosahedral viruses since the way by which SDS interacts with the surface of such viruses is poorly known. For this purpose, we examined the impact of SDS on the electrophoretic mobility of MS2 and Qβ phages, two related and structurally well‐defined bacteriophages , used here as model of non‐enveloped icosahedral viruses.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that SDS concentration in the BGE is a key parameter for such analyses, which must be high enough to solve problems of irreproducibility, but not too much to preserve the integrity of viral particles . The optimal compromise was generally found around the critical micellar concentration (CMC) of SDS.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, Oita et al . reported a study on the effect of SDS on CZE of poliovirus and its subviral particles obtained by thermal conversion . They showed that SDS, also above the CMC, is actively involved in the electrophoretic behavior of poliovirus.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

et al. 2017

View full text Add to dashboard Cite

SDS is commonly employed as BGE additive in CZE analysis of non-enveloped icosahedral viruses. But the way by which SDS interacts with the surface of such viruses remains to date poorly known, making complicate to understand their behavior during a run. In this article, two related bacteriophages, MS2 and Qβ, are used as model to investigate the migration mechanism of non-enveloped icosahedral viruses in SDS-based CZE. Both phages are characterized by similar size and surface charge but significantly different surface hydrophobicity (Qβ > MS2, where '>' means 'more hydrophobic than'). By comparing their electrophoretic mobility in the presence or not of SDS on both sides of the CMC, we show that surface hydrophobicity of phages is a key factor influencing their mobility and that SDS-virus association is driven by hydrophobic interactions at the surface of virions. The CZE analyses of heated MS2 particles, which over-express hydrophobic domains at their surface, confirm this finding. The correlations between the present results and others from the literature suggest that the proposed mechanism might not be exclusive to the bacteriophages examined here.

show abstract

Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

et al. 2017

View full text Add to dashboard Cite

show abstract

Sixteen capillary electrophoresis applications for viral vaccine analysis

et al. 2021

View full text Add to dashboard Cite

A broad range of CE applications from our organization is reviewed to give a flavor of the use of CE within the field of vaccine analyses. Applicability of CE for viral vaccine characterization, and release and stability testing of seasonal influenza virosomal vaccines, universal subunit influenza vaccines, Sabin inactivated polio vaccines (sIPV), and adenovirus vector vaccines were demonstrated. Diverse CZE, CE‐SDS, CGE, and cIEF methods were developed, validated, and applied for virus, protein, posttranslational modifications, DNA, and excipient concentration determinations, as well as for the integrity and composition verifications, and identity testing (e.g., CZE for intact virus particles, CE‐SDS application for hemagglutinin quantification and influenza strain identification, chloride or bromide determination in process samples). Results were supported by other methods such as RP‐HPLC, dynamic light scattering (DLS), and zeta potential measurements. Overall, 16 CE methods are presented that were developed and applied, comprising six adenovirus methods, five viral protein methods, and methods for antibodies determination of glycans, host cell‐DNA, excipient chloride, and process impurity bromide. These methods were applied to support in‐process control, release, stability, process‐ and product characterization and development, and critical reagent testing. Thirteen methods were validated. Intact virus particles were analyzed at concentrations as low as 0.8 pmol/L. Overall, CE took viral vaccine testing beyond what was previously possible, improved process and product understanding, and, in total, safety, efficacy, and quality.

show abstract

Affinity capillary electrophoresis to evaluate the complex formation between poliovirus and nanobodies

Halewyck

Schotte

Oita

et al. 2014

J of Separation Science

Self Cite

View full text Add to dashboard Cite

It was demonstrated that nanobodies with an in vitro neutralizing activity against poliovirus type 1 interact with native virions. Here, the use of capillary electrophoresis was investigated as an alternative technique for the evaluation of the formation of nanobody-poliovirus complexes, and therefore predicting the in vitro neutralizing activity of the nanobodies. The macromolecules are preincubated offline in a specific nanobody-to-virus ratio and analyzed by capillary electrophoresis with UV detection. At low nanobody-to-virus ratios, a clear shift in migration time of the viral peak was observed. A broad peak was obtained, indicating the presence of a heterogeneous population of nanobody-virion complexes, caused by the binding of different numbers of nanobodies to the virus particle. At elevated nanobody-to-virus ratios, a cluster of peaks appeared, showing an additional increase in migration times. It was shown that, at these high molar excesses, aggregates were formed. The developed capillary electrophoresis method can be used as a rapid, qualitative screening for the affinity between poliovirus and nanobodies, based on a clearly visible and measurable shift in migration time. The advantages of this technique include that there is no need for antigen immobilization as in enzyme-linked immunosorbent assays or surface plasmon resonance for the use of radiolabeled virus or for the performance of labor- and time-intensive plaque-forming neutralization assays.

show abstract

The effect of anionic surfactant on poliovirus particles during capillary electrophoresis

Cited by 5 publications

References 46 publications

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

Sixteen capillary electrophoresis applications for viral vaccine analysis

Affinity capillary electrophoresis to evaluate the complex formation between poliovirus and nanobodies

Contact Info

Product

Resources

About