The effect of analgesia with omnopon and dexketoprofen on the endocytic activity of phagocytes of different localization on the surgical tumor resection model

Abstract

We aimed to compare the effect of anesthesia with opioid analgesics omnopon and non-selective COX-2 inhibitor dexketoprofen on the endocytic activity of phagocytes of different localization sites on the model of surgical tumor removal. The study used 50 C57/black mice, which were transplanted with Lewis lung carcinoma in the hind paw pad. After 22 days the tumor paw was amputated. Analgesics (omnopon 10 mg/kg, dexketoprofen – 20 mg/kg) was administered 30 minutes before the operation and once per day for 3 days after surgery. Assessment of endocytic activity of phagocytes was performed by flow cytometry before the surgery, at days 1 and 3 after the surgery. It was found that dexketoprofen analgesia maintain the endocytic activity of blood and spleen phagocytes in the postoperative period. At day 3 postsurgery in dexketoprofen- treated animals phagocytic activities of blood and spleen granulocytes were higher compared to the group receiving opioid analgesia by 70% and 86% respectively. Phagocytic indices of blood and spleen monocytes were also 2 times higher at dexketoprofen-treated mice. Thus, dexketoprofen analgesia maintains the activity of blood and spleen phagocytes in mice after the surgical tumor removal at a much higher level as compared with the omnopon analgesia.