The effect of an isoflavonid-rich liquorice extract on fermentation, methanogenesis and the microbiome in the rumen simulation technique

Ramos-Morales, E.; Rossi, Giulia; Cattin, M; Jones, Eleanor; Bragança, Radek; Newbold, C. J.

doi:10.1093/femsec/fiy009

Cited by 26 publications

(11 citation statements)

References 47 publications

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“…Regarding the community composition, garlic oil treated samples of rumen fluid exhibited a unique archaeal community composition in DGGE analysis ( Patra and Yu, 2015 ). To our knowledge effects of citrus extract on the rumen archaeal community have not been investigated yet, however, other flavonoid-containing plant compounds may reduce archaeal richness and evenness ( Oh et al, 2017 ) or affect the major groups of methanogens ( Ramos-Morales et al, 2018 ). The high accordance of the results of both methods, which are also based on different primer pairs underline the reliability of the obtained results in the present study and the amplicon sequencing approach offers a detailed investigation on the effect of the experimental compound on the archaeal community.…”

Section: Discussionmentioning

confidence: 99%

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Eger

Graz²,

Riede

et al. 2018

Front. Microbiol.

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The reduction of methane emissions by ruminants is a highly desirable goal to mitigate greenhouse gas emissions. Various feed additives have already been tested for their ability to decrease methane production; however, practical use is often limited due to negative effects on rumen fermentation or high costs. Organosulphur compounds from garlic (Allium sativum) and flavonoids have been identified as promising plant-derived compounds which are able to reduce methane production. Here, we evaluated the effects of a combination of garlic powder and bitter orange (Citrus aurantium) extracts, Mootral, on ruminal methane production, ruminal fermentation and the community of methanogenic Archaea by using the rumen simulation technique as ex vivo model. The experiment consisted of an equilibration period of 7 days, an experimental period of 8 days and a withdrawal period of 4 days. During the experimental period three fermenters each were either treated as controls (CON), received a low dose of Mootral (LD), a high dose of Mootral (HD), or monensin (MON) as positive control. Application of Mootral strongly reduced the proportion of methane in the fermentation gas and the production rate of methane. Moreover, the experimental mixture induced a dose-dependent increase in the production rate of short chain fatty acids and in the molar proportion of butyrate. Some effects persisted during the withdrawal period. Both, single strand conformation polymorphism and Illumina MiSeq 16S rRNA amplicon sequencing indicated an archaeal community distinct from CON and MON samples in the LD and HD samples. Among archaeal families the percentage of Methanobacteriaceae was reduced during application of both doses of Mootral. Moreover, several significant differences were observed on OTU level among treatment groups and after withdrawal of the additives for LD and HD group. At day 14, 4 OTUs were positively correlated with methane production. In conclusion this mixture of garlic and citrus compounds appears to effectively reduce methane production by alteration of the archaeal community without exhibiting negative side effects on rumen fermentation.

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Section: Discussionmentioning

confidence: 99%

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Eger

Graz²,

Riede

et al. 2018

Front. Microbiol.

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“…Effluent was collected every day from days 6 to 8 in a 1.0-L container immersed in an ice water bath and filtered through a nylon cloth (Guangda Hengyi Co., Beijing, China) with an inner size of 8 cm × 12 cm and a pore size of 40 µm, as described in previous studies [15,31]. The residues were used to determine DM, organic matter (OM), NDF and CP as described in other studies [32,33], and filtrate was used to detect MPS.…”

Section: Samplingmentioning

confidence: 99%

Synchrony Degree of Dietary Energy and Nitrogen Release Influences Microbial Community, Fermentation, and Protein Synthesis in a Rumen Simulation System

et al. 2020

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Synchrony of energy and nitrogen release in rumen has been proposed to maximize ruminal microbial fermentation. However, the information regarding bacterial community composition and its metabolism under a higher or lower degree of synchronization is limited. In our study, a 0 to 6 h post-feeding infusion (first half infusion, FHI), 6 to 12 h post-feeding infusion (second half infusion, SHI), and 0 to 12 h post-feeding infusion (continuous infusion, CI) of maltodextrin were used to simulate varying degrees of synchronization of energy and nitrogen release in a rumen simulation system. In addition, the bacterial community, metabolite, enzyme activity, and microbial protein synthesis (MPS) were evaluated. Compared with the FHI and CI, the relative abundance of Fibrobacter, Ruminobacter, BF311, and CF231 decreased in the SHI, but that of Klebsiella and Succinivibrio increased in the SHI. The NH3-N and branched-chain volatile fatty acids were significantly higher, but propionate content and activities of glutamate dehydrogenase (GDH) and alanine dehydrogenase were significantly lower in the SHI than those in the FHI and CI. The SHI had lower MPS and less efficiency of MPS than the FHI and CI, which indicated that the SHI had a lower degree of synchronization. Correlation analysis showed that MPS was positively related to GDH activity and relative abundance of Fibrobacter but negatively related to NH3-N and relative abundance of Klebsiella. Therefore, a higher degree of synchronization of energy and nitrogen release increased MPS partly via influencing the bacterial community, metabolism, and enzyme activities of ammonia assimilation in the in vitro fermenters.

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“…Some studies have shown that variation in CH 4 emissions are not necessarily due to the changes in methanogen cell density, but rather the community structure of the methanogens [ 14 , 15 ]. Phytochemicals such as saponins, tannins and essential oils, have been used to manipulate rumen microbial populations and reduce CH 4 emissions from ruminants [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

The community structure and microbial linkage of rumen protozoa and methanogens in response to the addition of tea seed saponins in the diet of beef cattle

Tan

Ramírez-Restrepo

Shah

et al. 2020

J Animal Sci Biotechnol

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Background This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane (CH 4 ) production of six Belmont Red Composite beef steers fed tea seed saponins (TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d (BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d (TSS period), followed by the basal diet for 13 d without TSS (BDP post-control period). Results The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha . The addition of TSS was found to change the structure of methanogen community at the sub-genus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha , and Isotricha genus and SGMT clade methanogens were positively correlated with CH 4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH 4 emission. Conclusions These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.

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The effect of an isoflavonid-rich liquorice extract on fermentation, methanogenesis and the microbiome in the rumen simulation technique

Cited by 26 publications

References 47 publications

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Synchrony Degree of Dietary Energy and Nitrogen Release Influences Microbial Community, Fermentation, and Protein Synthesis in a Rumen Simulation System

The community structure and microbial linkage of rumen protozoa and methanogens in response to the addition of tea seed saponins in the diet of beef cattle

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