The effect of amino plasma‐enhanced chemical vapor deposition‐treated titanium surface on Schwann cells

Abstract

The surface modification of titanium and its alloys with amino group plasma-enhanced chemical vapor deposition has been proven to enhance the performance of implants on initial osteoblast bioactivity in vitro. However, scarce information on the effect of this kind of surface modification on nerve regeneration exists. In this study, the surface chemistry of pure Ti disks and surface-modified disks was examined using X-ray photoelectron spectroscopy. Cell counting kit 8 assay, 4,6-diamidino-2-phenylindole staining, flow cytometry, and scanning electron microscopy showed that either the p30% or cw 1 p30% mode-mediated surface significantly promote Schwann cell adhesion without any cytotoxicity compared with the pure Ti surface, and the cw 1 p30% group showed the best performance on cell adhesion. However, results of polymerase chain reaction and Western blot analyses showed that the mRNA and protein levels of glial cell-derived neurotrophic factor and nerve growth factor of the p30% and cw 1 p30% groups were lower than those of the Ti group at some time points. Generally, the results indicate that amino-functionalized Ti surfaces can promote Schwann cell adhesion without cytotoxicity, but this modification, in fact, inhibited the expression of the key growth factors GDNF and NGF of Schwann cells.

Surface Modification Techniques of Titanium and its Alloys to Functionally Optimize Their Biomedical Properties: Thematic Review

“…Scarce information exists on the effect of amino group (Du et al, 2016;Youn et al, 2019) Good controlling on composition and characteristics of the film, flexibility plasma-enhanced CVD on nerve regeneration. Hence, Zhao et al (2018) introduced the amino group onto the titanium disk. Although, it exhibited the best cell attachment performance, it inhibited the expression of the key growth factors like glial cellderived neurotrophic factor (GDNF) and neurotrophin nerve growth factor (NGF) in vitro, at least within a week.…”

Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells

“…In the present study, we synthesized an anodized oxide surface of TiO 2 nanotubes at the voltage of 30–50 V. The average tube diameter was found to increase with increasing anodizing voltage. There is a precise correlation between the anodization voltage and pore size, thus by varying the voltage substrates with different size scales can be fabricated [ 3 , 34 ]. The formation of the surface not only increased the surface area and roughness, but also provided a better surface for adhesion.…”

“…After culturing for 1, 4 and 7 days, total RNA was extracted from treated cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed into cDNA using a Revert Aid kit (Takara Bio, Otsu, Japan) according to the manufacturer’s instructions. PCR reactions were setup using SYBR Premix Ex Taq (Takara Bio) and performed with an Exicycler 96 real-time PCR system (Bioneer, Daejeon, Korea) [ 33 , 34 ]. The mRNA level of cells cultured on pure titanium foils was set as the baseline.…”

“…A0208, Biyuntian, China) and visualized with an ECL reagent (Qihai Biotech, China). The intensity of the bands, which were representative of protein levels, were assessed using Gel-Pro-Analyzer 3.0 (Media Cybernetics, Rockville, MD, USA).β-actin was used to normalize target proteins [ 33 , 34 ].…”

Preparation and properties of composite manganese/fluorine coatings on metallic titanium

“…Common methods include plasma spraying, chemical vapor deposition, ion implantation, the sol gel process, anodic oxidation and microarc oxidation. [2][3][4][5][6] Plasma spraying is the most widely used surface modication technology in the clinic. It uses a plasma arc as a heat source to heat some materials, such as alloys, ceramics and metals, to a molten or semimolten state and spray them at a high speed to the pretreated substrate surface to form a rmly attached coating.…”