The effect of alternative membrane system, sucrose, and culture methods under photosynthetic photon flux on growth and volatile compounds of mint in vitro

Oliveira, Thainá de; Balduino, Mayra Cristina Manoel; Carvalho, Alexandre Alves de; Bertolucci, Suzan Kelly Vilela; Cossa, Melvis Celeste Vilanculos; Coelho, Adriane Duarte; Leite, Jeremias José Ferreira; Pinto, José Eduardo Brasil Pereira

doi:10.1007/s11627-020-10147-z

Cited by 15 publications

(10 citation statements)

References 39 publications

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“…Several authors report that high sucrose concentrations in the medium are deleterious for photosynthetic activity [5,26]. However, sucrose has also been reported to stimulate photosynthesis in in vitro plantlets [27,28]. ROS formation can also be stimulated [29] or decreased [30] by sugar.…”

Section: Discussionmentioning

confidence: 99%

Low CO2 Levels Are Detrimental for In Vitro Plantlets through Disturbance of Photosynthetic Functionality and Accumulation of Reactive Oxygen Species

2022

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Photosynthesis of plantlets in tissue culture containers is not considered important, compared to photosynthesis of ex vitro plants, due to the exogenous source of carbohydrates present in tissue culture media. However, CO2 starvation can generate a burst of reactive oxygen species (ROS). We examined this phenomenon in tissue culture, since CO2 levels may become very low during the light period. The research was carried out with lily scales, regenerating adventitious bulblets, and with Arabidopsis seedlings. CO2 starvation was achieved by placing a small vial of concentrated KOH solution in the culture container. CO2 removal reduced the growth of regenerated lily bulblets by 33% or 23%, with or without sucrose in the medium, respectively. In Arabidopsis seedlings, CO2 removal decreased growth by 50% or 78% in the presence or absence of sucrose in the medium, respectively. Therefore, the addition of sucrose as a replacement for photosynthesis resulted in only partial recovery of growth. Staining with nitroblue tetrazolium (NBT) showed little to no ROS in ex vitro growing seedlings, while abundant ROS were detected in seedlings grown under in vitro CO2 starvation. Seedlings grown under normal tissue culture conditions (no CO2 withdrawal) showed low levels of ROS. In lily tissue culture, CO2 starvation decreased the maximum quantum efficiency of photosystem II (Fv/Fm) from 0.69 to 0.60, and in Arabidopsis from 0.76 to 0.62. Fv/Fm of ex vitro lily and Arabidopsis seedlings was 0.77 and 0.79, respectively. This is indicative of a disturbance in photosynthesis functionality and the occurrence of in vitro stress under reduced CO2 concentrations. We conclude that poor growth, in the absence of CO2, was partly due to strongly reduced photosynthesis, while the detrimental effects were most likely due to a burst of ROS.

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Section: Discussionmentioning

confidence: 99%

Low CO2 Levels Are Detrimental for In Vitro Plantlets through Disturbance of Photosynthetic Functionality and Accumulation of Reactive Oxygen Species

2022

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“…Quantitative analysis of the EO was performed by gas chromatography coupled with a flame ionization detector (GC-FID) in an Agilent ® 7890A system equipped with DB-Wax capillary column (30 m length x 0.25 mm internal diameter × film thickness of 0.25 μm) (Agilent J&W). The technical parameters of the chemical analysis were adjusted according to SILVA et al (2019) andOLIVEIRA et al (2021). Helium was used as carrier gas at 1.0 mL/min, temperatures of the injector and detector were maintained at 220 °C and 240 °C, respectively.…”

Section: Methodsmentioning

confidence: 99%

Seasonal variation in essential oil content and chemical profile of mint in southeast of Brazil

et al. 2021

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This study evaluated the influence of seasonality in essential oil (EO) chemical composition of Mentha x piperita L. and Mentha viridis L. grown in southeast of Brazil. Leaves were collected from November 2011 until August 2013, in the middle of the following seasons: Spring (November), Summer (February), Autumn (May) and Winter (August). EO extraction was carried out by hydrodistillation in Clevenger apparatus for 1 hour. Chemical composition of EO was analyzed by GC-FID and GC-MS. The EO content and its chemical composition were influenced by seasonality in both species. The highest EO content was obtained for M. x piperita during spring (4.26%) and for M. viridis during spring (3.30%) and summer (3.70%). Mentha x piperita increased menthol (16.31 to 41.26%), neomenthol (3.02 to 6.39%) and menthone (5.56 to 41.58%) contents during spring and summer, respectively. Whereas, M. viridis EO content did not show a quality response pattern in relation to seasonality. Therefore, harvest time for M. x piperita is recommended during spring, and for M. viridis is recommended during spring and summer.

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“…Lazzarini et al (2019) reported that an alternative system with 4 membranes and the type of explant significantly influenced the growth and volatile fraction of Lippia gracilis. Rocha et al (2022) arvensis and M. viridis ((de Oliveira et al, 2021).…”

Section: In Vitro Growthmentioning

confidence: 99%

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

Coelho

Leite²,

Santos³

et al. 2022

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The objective of this study was to evaluate the effect of the use of different natural ventilation systems on the growth and accumulation of total phenolic compounds in Aloysia gratissima. Nodal and apical segments with 1 pair of leaves were inoculated in flasks containing ½ MS culture medium without sucrose and supplemented with 0.1 mg L-1 indole-butyric acid. The cultivation systems included a conventional system (NMS) and alternative membrane systems with 1 (AMS1), 2 (AMS2) and 4 (AMS4) low-cost membranes. After 30 days, the in vitro growth, leaf area, dry weight and accumulation of phenolic compounds were evaluated. Plantlets from apical segments showed superior results in all variables analyzed. The use of the NMS compromised plantlet growth. However, improved results were obtained with the use of porous membranes, with the best growth observed in AMS4. The leaf area of plantlets originating from apical segments was 3.03 times greater than that of plantlets from nodal segments. Plantlets in the NMS had the lowest values of leaf area, root length, number of leaves and dry weight. However, the use of membranes allowed higher growth. The AMS4 treatment increased the leaf dry weight accumulation by 6.13-fold compared to that obtained with the NMS treatment. The accumulation of total phenolic compounds increased with the use of greater numbers of porous membranes. The use of apical segments and lids with 4 porous membranes is recommended for micropropagation of the species. The use of an alternative membrane system positively influences the accumulation of total phenolic compounds.

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The effect of alternative membrane system, sucrose, and culture methods under photosynthetic photon flux on growth and volatile compounds of mint in vitro

Cited by 15 publications

References 39 publications

Low CO2 Levels Are Detrimental for In Vitro Plantlets through Disturbance of Photosynthetic Functionality and Accumulation of Reactive Oxygen Species

Low CO2 Levels Are Detrimental for In Vitro Plantlets through Disturbance of Photosynthetic Functionality and Accumulation of Reactive Oxygen Species

Seasonal variation in essential oil content and chemical profile of mint in southeast of Brazil

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

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