The Effect of Alternating Current Iontophoresis on Rats with the Chronic Constriction Injury to the Infraorbital Nerve

Yamazaki, Yoshio; Umino, Masahiro; Fukayama, Haruhisa; Shimada, Masahiko

doi:10.1155/2012/405292

Cited by 2 publications

(2 citation statements)

References 21 publications

(22 reference statements)

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“…Potential side effects from the long-term use of electrical fields on living tissue are also an unknown variable. 22,23 Recently micrometer-sized sharp structures called microneedles have been developed to precisely target the skin for localized, painless, and minimally invasive delivery of drugs and vaccines. 24,25 Among the different approaches that exist to deliver drugs using microneedles, the coated microneedle approach is attractive because of its simple implementation and potential thermal stability offered because the drug is in a solid dry state on the microneedle.…”

Section: ■ Introductionmentioning

confidence: 99%

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Microprecision Delivery of Oligonucleotides in a 3D Tissue Model and Its Characterization Using Optical Imaging

Luo

et al. 2013

Mol. Pharmaceutics

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Despite significant potential of oligonucleotides (ONs) for therapeutic and diagnostic applications, rapid and widespread intracellular delivery of ONs in cells situated in tissues such as skin, head and neck cavity, and eye has not been achieved. This study was aimed at evaluating the synergistic combination of microneedle (MN) arrays and biochemical approaches for localized intratissue delivery of oligonucleotides in living cells in 3D tissue models. This synergistic combination was based on the ability of MNs to precisely deliver ONs into tissues to achieve widespread distribution, and the ability of biochemical agents (streptolysin O (SLO) and cholesterol conjugation to ONs) to enhance intracellular ON delivery. The results of this study demonstrate that ON probes were uniformly coated on microneedle arrays and were efficiently released from the microneedle surface upon insertion in tissue phantoms. Co-insertion of microneedles coated with ONs and SLO into 3D tissue models resulted in delivery of ONs into both the cytoplasm and nucleus of cells. Within a short incubation time (35 min), ONs were observed both laterally and along the depth of a 3D tissue up to a distance of 500 μm from the microneedle insertion point. Similar widespread intratissue distribution of ONs was achieved upon delivery of ON-cholesterol conjugates. Uniformity of ON delivery in tissues improved with longer incubation times (24 h) postinsertion. Using cholesterol-conjugated ONs, delivery of ON probes was limited to the cytoplasm of cells within a tissue. Finally, delivery of cholesterol-conjugated anti-GFP ON resulted in reduction of GFP expression in HeLa cells. In summary, the results of this study provide a novel approach for efficient intracellular delivery of ONs in tissues.

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Section: ■ Introductionmentioning

confidence: 99%

“…Although iontophoresis and electrophoresis are attractive approaches, their clinical application has yet to be realized. Potential side effects from the long-term use of electrical fields on living tissue are also an unknown variable. , …”

Section: Introductionmentioning

confidence: 99%

Microprecision Delivery of Oligonucleotides in a 3D Tissue Model and Its Characterization Using Optical Imaging

Luo

et al. 2013

Mol. Pharmaceutics

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The Immediate Effects of Lidocaine Iontophoresis using Interferential Current on Pressure Sense Threshold and Tactile Sensation

2016

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Iontophoresis is the noninvasive delivery of ions using direct current. The direct current has some disadvantages such as skin burning. Interferential current is a kind of alternating current without limitations of direct current; so the purpose of this study is to investigate and compare the effects of lidocaine, interferential current and lidocaine iontophoresis using interferential current. 30 healthy women aged 20-24 years participated in this randomized clinical trial study. Pressure, tactile and pain thresholds were evaluated before and after the application of treatment methods. Pressure, tactile and pain sensitivity increased significantly after the application of lidocaine alone (p < 0.005) and lidocaine iontophoresis using interferential current (p < 0.0001). Lidocaine iontophoresis using interferential current can increase perception threshold of pain, tactile stimulus and pressure sense more significantly than lidocaine and interferential current alone.

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The Effect of Alternating Current Iontophoresis on Rats with the Chronic Constriction Injury to the Infraorbital Nerve

Cited by 2 publications

References 21 publications

Microprecision Delivery of Oligonucleotides in a 3D Tissue Model and Its Characterization Using Optical Imaging

Microprecision Delivery of Oligonucleotides in a 3D Tissue Model and Its Characterization Using Optical Imaging

The Immediate Effects of Lidocaine Iontophoresis using Interferential Current on Pressure Sense Threshold and Tactile Sensation

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