The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Abstract

INTRODUCTION: The aim of this study is to investigate the effects of obstructive jaundice on the liver and effectivity of alpha-lipoic acid on liver damage and oxidative stress. MATERIALS AND METHODS: Thirty-six male Sprague-Dawley rats were divided into 3 groups per 12 animals, namely into Group I (control group): the bile duct was only mobilized by laparotomy, Group II (bile duct ligation group-BDL): the common bile duct was closed with clips and OJ was caused after laparotomy, and Group III (bile duct ligation and alpha-lipoic acid group-BDL+LA): after closing the common bile duct, LA was administered in an intramuscular dose of 50 mg/kg for 10 days. On the 10th day, malondialdehyde, glutathione and superoxide dismutase levels were measured in liver and histopathological evaluation was performed. RESULTS: AST (U/L)/ALT(U/L) in groups I, II and III were 155.33/51.83, 445.28/165.89, 380.78/173.33, respectively (p < 0.005). Superoxide dismutase and glutathione levels were lower in patient groups than in the control group (0.31 μl/g vs 0.36 μl/g; p < 0.05). After the lipoic acid treatment, none of the biochemical markers of liver improved. Only the increase in superoxide dismutase (0.31 μl/g and 0.34 μl/g in groups II and III, respectively) and glutathione levels (0.16 μl/g and 0.22 μl/g in groups II and III, respectively) was statistically signifi cant (p < 0.05). CONCLUSIONS: Histopathological damage was statistically signifi cantly decreased and antioxidant levels were statistically signifi cantly increased after LA treatment (Tab. 1, Fig. 6, Ref. 23).

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

“…Furthermore, we found that ALA significantly enhanced GPx, SOD, and CAT activity, whereas it inhibited MDA production under heat stress. This may partly explain the anti-oxidative action of ALA in vivo reported in previous studies [32,33]. Based on these results, we speculated that HS caused a decrease in anti-oxidative capacity and disrupted the balance between oxidative and antioxidative status.…”

Comparative Study of the Results of Operations in Patients with Tumor and Non-Tumor Obstructive Jaundice Who Received and Did Not Receive Antioxidant Therapy for the Correction of Endotoxemia, Glycolysis, and Oxidative Stress

“…Thus, in the present study, we analyzed the treatment outcomes of patients with surgical jaundice from the standpoint of the dynamic characteristics of the free radical processes involved. The relationship between free radical processes indicators and the severity of OJ was confirmed not only by our study but also by other studies conducted in different countries [ 21 , 22 , 34 , 35 , 36 , 42 , 43 , 44 , 45 , 46 , 47 ]. In animal models and in patients with OC, a marked increase in plasma levels of oxidative stress markers was recorded, while the level of antiperoxidase activity paradoxically decreased.…”

Oxidative Stress and Free Radical Processes in Tumor and Non-Tumor Obstructive Jaundice: Influence of Disease Duration, Severity and Surgical Treatment on Outcomes

“…The analysis of the samples obtained proved that FRP indices differ significantly between healthy individuals and patients with obstructive jaundice. Therefore, it is not surprising that, based on the earlier studies, other researchers are trying to find drugs to normalize free radical processes in patients with jaundice [ 31 , 32 , 33 , 34 ].…”