The effect of albumin on astrocyte energy metabolism is not brought about through the control of cytosolic Ca2+ concentrations but by free-fatty acid sequestration

Tabernero, Arantxa; Medina, Ana Maria; Sánchez‐Abarca, Luis Ignacio; Lavado, Eva; Medina, José M.

doi:10.1002/(sici)1098-1136(19990101)25:1<1::aid-glia1>3.0.co;2-2

Cited by 43 publications

(18 citation statements)

References 44 publications

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“…Cells were plated onto poly-L-lysine (10 g/ml)-coated Petri dishes at a density of 1.0 ϫ 10 5 cells/cm 2 . Albumin Loading and Unloading from Astrocytes-14 DIV astrocytes were loaded at 37°C in Elliot buffer (21) (11 mM sodium phosphate, 122 mM NaCl, 4.8 mM KCl, 0.4 mM KH 2 PO 4 , 1.2 mM MgSO 4 , and 1.3 mM CaCl 2 , pH 7.4) containing 5 mM glucose and 2% (w/v) bovine serum albumin conjugated to fluorescein isothiocyanate (FITC-BSA; Sigma) as described (22). After 10 min, cells were washed three times at 4°C with Elliot buffer and washed an additional three times with Elliot buffer plus 2% (w/v) BSA.…”

Section: Methodsmentioning

confidence: 99%

“…Statistical analyses were carried out using ANOVA followed by the Scheffe F-test. (22) have previously shown that astrocytes internalize BSA by a temperature-dependent process. To gain insight into the mechanism by which albumin is taken up by astrocytes, cells were exposed to FITC-BSA in the absence or in the presence of PAO, an inhibitor of receptor-mediated endocytosis (28).…”

Section: Electrophoretic Analysis Of Fitc-bsa and Oleic Acid Releasedmentioning

confidence: 99%

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Transcytosis of Albumin in Astrocytes Activates the Sterol Regulatory Element-binding Protein-1, Which Promotes the Synthesis of the Neurotrophic Factor Oleic Acid

Tabernero

Velasco

Granda

et al. 2002

Journal of Biological Chemistry

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We have recently reported that albumin, a serum protein present in the developing brain, stimulates the synthesis of oleic acid by astrocytes, which promotes neuronal differentiation. In this work, we gain insight into the mechanism by which albumin induces the synthesis of this neurotrophic factor. Our results show that astrocytes internalize albumin in vesicle-like structures by receptor-mediated endocytosis. Albumin uptake was followed by transcytosis, including passage through the endoplasmic reticulum, which was required to induce the synthesis of oleic acid. Oleic acid synthesis is feedback-regulated by the sterol regulatory element-binding protein-1, which induces the transcription of stearoylCoA 9-desaturase, the key rate-limiting enzyme for oleic acid synthesis. In our research, the presence of albumin activated the sterol regulatory element-binding protein-1 and increased stearoyl-CoA 9-desaturase mRNA. Moreover, when the activity of sterol regulatory element-binding protein-1 was inhibited by overexpression of a truncated form of this protein, albumin did not affect stearoyl-CoA 9-desaturase mRNA, indicating that the effect of albumin is mediated by this transcription factor. The effect of albumin was abolished when traffic to the endoplasmic reticulum was prevented or when albumin was accompanied with oleic acid. In conclusion, our results suggest that the transcytosis of albumin includes passage through the endoplasmic reticulum, where oleic acid is sequestrated, initiating the signal cascade leading to an increase in its own synthesis.Astrocytes, the main glial cell population in the central nervous system, play a major role in supporting the development of neurons. In fact, astrocytes synthesize and release extracellular matrix proteins and adhesion molecules, which participate not only in the migration of neurons but also in the formation of neuronal aggregates. In addition, astrocytes produce a broad spectrum of growth factors and cytokines, which can regulate the morphology, proliferation, differentiation, and survival of neurons (for a review, see Ref.

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Section: Methodsmentioning

confidence: 99%

Section: Electrophoretic Analysis Of Fitc-bsa and Oleic Acid Releasedmentioning

confidence: 99%

Transcytosis of Albumin in Astrocytes Activates the Sterol Regulatory Element-binding Protein-1, Which Promotes the Synthesis of the Neurotrophic Factor Oleic Acid

Tabernero

Velasco

Granda

et al. 2002

Journal of Biological Chemistry

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show abstract

“…Albumin has been reported to play an important role in astrocyte functions. It is shown that albumin affects metabolism of cultured astrocytes (Tabernero et al, 1999). Albumin up on transcytosis into cultured astrocytes stimulates the synthesis of neurotrophic factor oleic acid which promotes neuronal differentiation (Tabernero et al, 2002).…”

Section: Direct Neuroprotection By Albumin: Mechanismsmentioning

confidence: 99%

Endogenous Agents That Contribute to Generate or Prevent Ischemic Damage

Piazza¹,

Scarpati²

2012

Advances in the Preclinical Study of Ischemic Stroke

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“…Tabernero and et al in a study showed that in pathological condition, albumin enter brain cells and may help maintain neuronal metabolism by increasing mechanisms. 8 Belayev and et al in a study showed that treatment with moderate dose of albumin cause to recovery in neurologic condition, decreasing infarct volume and brain edema. 9 Albumin is a protein soluble in water and one of the most important proteins in the plasma, which accounts for 65-55% of total protein in the body.…”

Section: Introductionmentioning

confidence: 97%

Albumin impact on clinical practice and complications of ischemic stroke in patients with stroke

Abbasi

Fattahzadeh-Ardalani

Safarnejad

et al. 2016

Int J Basic Clin Pharmacol

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The effect of albumin on astrocyte energy metabolism is not brought about through the control of cytosolic Ca2+ concentrations but by free-fatty acid sequestration

Cited by 43 publications

References 44 publications

Transcytosis of Albumin in Astrocytes Activates the Sterol Regulatory Element-binding Protein-1, Which Promotes the Synthesis of the Neurotrophic Factor Oleic Acid

Transcytosis of Albumin in Astrocytes Activates the Sterol Regulatory Element-binding Protein-1, Which Promotes the Synthesis of the Neurotrophic Factor Oleic Acid

Endogenous Agents That Contribute to Generate or Prevent Ischemic Damage

Albumin impact on clinical practice and complications of ischemic stroke in patients with stroke

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