The Effect of Al3+ and Hg2+ on Glucose 6-Phosphate Dehydrogenase From Capoeta Umbla Kidney

Abstract

Abstract. Glucose 6-phosphate dehydrogenase (EC 1.1.1.49; G6PD) is an important enzyme found in all mammal tissues, and produces NADPH in the metabolism. NADPH provides a reductive potential to maintain a balanced redox state within the cell. The aim of this study was to purify G6PD from Capoeta umbla kidney and determination of inhibition or activation effects of aluminium and mercury on enzyme activity. In this purpose, glucose 6-phosphate dehydrogenase was purified from Capoeta umbla kidney by using preparation of homogenate, ammonium sulphate precipitation and 2',5'-ADP Sepharose 4B affinity chromatography. Molecular weight of the enzyme was determined on sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE) and the purified enzyme showed a single band on the gel with a molecular weight of 75 kDa. Moreover, K i constants of Al 3+ and Hg 2+ were found as 0.98 ± 0.084 and 0.57 ± 0.019 mM, respectively. In conclusion, affinity of the Hg 2+ to the enzyme was higher than Al 3+

Determination of Some Metal Levels in Water, Sediment and Fish Species of Tercan Dam Lake, Turkey

“…It was determined that the metal accumulation in the muscle tissues of Additionally, it was determined that the accumulation of As, Cr, and Ni metals in the muscle tissues of the common carp were lower than the analysis limitations (Table 9). There have been numerous research studies conducted in various parts of the world on the accumulation of metals in fish tissues and their detrimental effects on the tissues (Moiseenko and Kudryavtseva, 2001;Uysal et al, 2008;Kirici et al, 2013bKirici et al, , 2016bEl-Moselhy et al, 2014;Baharom and Ishak, 2015;Bosch et al, 2016;La Colla et al, 2017. For instance, in a study conducted on Avşar Lake (Turkey), Öztürk et al (2009) examined the metal accumulation concentrations in the muscle, liver, and gill tissues of the Carp, and as the conclusion of the study, it was determined that the Fe metal had the highest accumulation level while Cd had the least.…”

Toxic effects of copper sulphate pentahydrate on antioxidant enzyme activities and lipid peroxidation of freshwater fish Capoeta umbla (Heckel, 1843) tissues

“…Murat River is one of the most important large and long (722 km) tributary of the Euphrates River in South East Anatolia of Turkey. The distribution area of Murat River is upper basins systems of the Euphrates and Tigris River (Koyun, 2011;Kirici et al, 2016). After capturing, fish were transferred to at the Aquarium Fish Rearing Facility of the Department of Fishery Science, Agricultural Faculty at Bingöl University and fish were held in a 600 L water valum fiber glass tank.…”

Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity

“…It is well known that almost all metals show their effects on various enzymes in the metabolism. Particularly, some enzymes are target for this substances such as carbonic anhydrase, glutathione reductase, G6PD and paraoxonase (Ekinci et al, 2007;Alici et al, 2008;Ekinci and Beydemir, 2009;Şentürk et al, 2011;Söyüt et al, 2012;Türkeş et al, 2015;Kirici et al, 2016a).…”

“…Also, G6PD-metal interactions have been investigated on a variety of fishes in vitro for a long time (Cankaya et al, 2011;Hu et al, 2013;Comakli et al, 2015;Kirici et al, 2016a). In our study, we investigated the toxicological effects of some metal ions, including Ag + , Cd 2+ , Cu 2+ , Fe 2+ , Ni 2+ and Pb 2+ on the G6PD enzyme purified from the liver and gill of freshwater fish Capoeta umbla using the affinity chromatography method under in vitro conditions.…”