The Effect of Age and Occupation on the Seroprevalence of <i>Helicobacter pylori</i> Infection

Perez-Perez, Guillermo I.; Marrie, Thomas J.; Inouye, H; Shimoyama, Takashi; Marshall, Gary S.; Meiklejohn, Gordon; Blaser, M. J.

doi:10.1155/1992/831523

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…Results for IgA and IgG were expressed as OD ratios (ODRs) (11,42), and for IgM, the results were expressed as OD values (35). The sensitivity and specificity of testing with this antigen were Ͼ95% (11,64), and the intra-and interassay variations have been shown to be Ͻ5% (11,47). The presence of antibodies to the H. pylori CagA antigen was determined by an ELISA to detect serum IgG antibodies against orv220, a molecular weight 65,000 recombinant CagA protein purified from Escherichia coli (Acambis, Cambridge, Mass.…”

Section: Methodsmentioning

confidence: 99%

“…Test sera were diluted 1:100 and assayed in duplicate. This ELISA has a sensitivity of 94.4% and a specificity of 92.5% (47) and also has been validated in multiple diverse populations (23,43,68). H. pylori culture-positive persons may be seropositive in the CagA assay in the absence of response to the WC antigen (54).…”

Section: Methodsmentioning

confidence: 99%

“…Seroreversion was defined as the loss of positivity from previously positive values. For studies of the women of childbearing age, the definition of serostatus was based on prior definitions for adults (42,47). Serum from 2-month-old infants in the birth cohort also was used as a proxy for maternal serum IgG levels.…”

Section: Methodsmentioning

confidence: 99%

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Transient and PersistentHelicobacter pyloriColonization in Native American Children

et al. 2003

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Helicobacter pylori is chiefly acquired in childhood, but the exact timing of acquisition is not well understood. The main goal of this study was to assess H. pylori acquisition in a pediatric population. We studied two cohorts of Native American children: a birth cohort of 50 children and 58 older children (mean age, 53 months). We measured serum immunoglobulin G (IgG), IgM, and IgA antibodies to H. pylori whole-cell antigen and IgG antibodies to CagA. Among 44 birth cohort children monitored for more than 12 months, 24 (54.5%) had seroconversions, 7 (15.9%) were transient, and 17 (38.6%) were persistent. Among the older children, 49 (84.5%) of the 58 children were monitored for 1 year; 34 (69.4%) had H. pylori antibodies at study entry. During the next year, 7 (20.6%) children seroreverted, and of 15 initially negative children, 5 (33.3%) seroconverted. In both groups, evaluation of CagA antibodies increased the sensitivity of H. pylori detection. Serum pepsinogen I (PGI) levels in H. pylori-negative children rose significantly until age 6 months and remained constant for the next 19 months. At the time of H. pylori seroconversion, PGI peaked to levels significantly higher than in the never-seroconverted (P ‫؍‬ 0.02) and the pre-seroconverted (P ‫؍‬ 0.03) children, but then declined to levels paralleling those of H. pylori-negative children. Thus, H. pylori acquisition, accompanied by a transient PGI increase, was frequent in this population, especially in the second and third years of life, but often was brief.Gastric colonization by Helicobacter pylori is highly prevalent throughout the world (3,15,41), and the organism is predominantly acquired during childhood (19,34). In developing countries, more than 80% of adults are colonized with H. pylori, and more than 50% of children become colonized before the age of 10 years compared to 30% of the adults and 10% of children in developed countries like the United States (15-17, 50, 66). With socioeconomic development, the prevalence of H. pylori has declined (26,48,50). H. pylori colonization generally persists throughout life, except in persons treated with antibiotics or those who develop atrophic gastritis, usually late in life (18,70). Although most adults have no clinical consequences of H. pylori colonization, it is a risk factor for peptic ulcer disease (7, 15, 37) and noncardia gastric adenocarcinoma (22,38). While colonization of adults with H. pylori almost always persists (26,42,48), there has been evidence for both transient and persistent colonization in children (19, 65) and in experimentally challenged nonhuman primates (12). The relative frequency of transient H. pylori acquisition among children in developing countries has been only partially characterized (29,58,65), and there is little information about gastric physiology in children in relation to H. pylori acquisition (58). An important question is whether pepsinogen production, reflecting gastric acidity (71), is affected by the H. pylori status of the child.The Native American Apache people...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Transient and PersistentHelicobacter pyloriColonization in Native American Children

et al. 2003

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“…This single high dilution was selected to dilute out nonspecific antibodies; its accuracy for screening has been previously described (18,21). Appropriate blanks and positive and negative controls were included in each assay as previously described (23,24).…”

Section: Methodsmentioning

confidence: 99%

Antigenic characterization of Helicobacter pylori strains from different parts of the world

Höök‐Nikanne

Pérez-Pérez

Blaser

1997

Clin Diagn Lab Immunol

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Although Helicobacter pylori is considered to be relatively homogeneous at the phenotypic level, our aim was to describe its antigenic heterogeneity and to examine differences in host response. Whole-cell lysates of H. pylori strains originally isolated from persons from Africa, the People's Republic of China, Japan, Peru, Thailand, or the United States or from monkeys were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoblots were performed by using sera from H. pylori-infected persons from different areas of the world and rabbit immune sera against H. pylori antigens. Specific H. pylori antibody responses in persons from the United States and the People's Republic of China were analyzed by enzyme-linked immunosorbent assay with antigens prepared from U.S. or Chinese strains. Despite diverse origins, the strains showed conserved major bands of 84, 60, 56, 31, and 25 kDa. Although there were clear differences in minor bands, there was no obvious geographic pattern. The anti-CagA serum recognized 120-to 140-kDa bands in cagA ؉ strains from around the world. Although antigenic preparations from individual U.S. or Chinese strains were not optimally sensitive for serologic detection of infection in the heterologous country, use of pools of strains largely overcame this phenomenon. We conclude that conserved H. pylori antigens exist and are recognized by sera from persons from many parts of the world. The heterogeneity of H. pylori antigens and the serological responses of infected hosts is not fully explained by geographic differences. Use of pools may allow development of antigens for serologic testing in any country.

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“…Serum samples were collected from healthy persons (who were not endoscopy subjects) in 8 populations (representing 5 continents) and were selected from stored sera previously collected. The 8 populations (and numbers of subjects) were from: Soongnern District, Thailand (n 5 130) (Pérez-Pérez et al, 1990); Lima, Peru (n 5 178); Shandong province, China (n 5 177) (Zhang et al, 1996); Manitoba, Canada (n 5 469) (Pérez-Pérez et al, 1992); Amsterdam, The Netherlands (n 5 105) (Kuipers et al, 1995); and New Zealand (n 5 579) (Fraser et al, 1996). From New Zealand, 3 different ethnic groups were examined: Caucasian (n 5 190), Pacific Islander (n 5 194) and Maori (n 5 195).…”

Section: Populationsmentioning

confidence: 99%

Country-specific constancy by age incagA+ proportion ofHelicobacter pylori infections

et al. 1997

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Helicobacter pylori strains may be either cagA 1 or cagA 2 , and in logitudinal studies, infection with a cagA 1 strain has been associated with increased risk for the development of atrophic gastritis and cancer of the distal stomach. We sought to determine the relative proportion of strains producing CagA in different geographic locales, and the extent to which CagA seroprevalence varied in countries with different gastric and esophageal cancer rates. Using an enzyme-linked immunosorbent assay (ELISA) to detect serum IgG to CagA, we examined sera from 468 asymptomatic H. pylori-infected adults from Canada, Peru, China, Thailand, The Netherlands and 3 different ethnic groups in New Zealand. The CagA seroprevalence in Peru and Thailand (82.2% and 78.8%, respectively) were each substantially higher than for the Chinese (37.9%), Canadian (41.9%), Dutch (39.0%) and New Zealand (28.2%) subjects, but within each population, rates were relatively constant across gender and age groups. Reported gastric but not esophageal cancer rates for the 8 studied populations were significantly associated with H. pylori seroprevalence. Variation in CagA positivity rates was not significantly associated with variation in either gastric or esophageal cancer rates. Our data suggest that CagA seroprevalence is not the major factor influencing gastric cancer rates. Int.

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The Effect of Age and Occupation on the Seroprevalence of Helicobacter pylori Infection

Cited by 5 publications

References 17 publications

Transient and PersistentHelicobacter pyloriColonization in Native American Children

Transient and PersistentHelicobacter pyloriColonization in Native American Children

Antigenic characterization of Helicobacter pylori strains from different parts of the world

Country-specific constancy by age incagA+ proportion ofHelicobacter pylori infections

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