The effect of administration of copper nanoparticles to chickens in drinking water on estimated intestinal absorption of iron, zinc, and calcium

Abstract

Copper nanoparticles used as a dietary supplement for poultry could affect the absorption of mineral elements. Hence the aim of the study was to determine the effect of administration of copper nanoparticles to chickens in drinking water on intestinal absorption of iron, zinc, and calcium. The experiment was carried out on 126 chicks assigned to seven experimental groups of 18 birds each (3 replications of 6 individuals each). The control group (G-C) did not receive copper nanoparticles. Groups: Cu-5(7), Cu-10(7), and Cu-15(7) received gold nanoparticles in their drinking water in the amounts of 5 mg/L for group Cu-5(7), 10 mg/L for group Cu-10(7), and 15 mg/L for group Cu-15(7) during 8 to 14, 22 to 28, and 36 of 42 days of the life of the chicks. The birds in groups Cu-5(3), Cu-10(3), and Cu-15(3) received copper nanoparticles in the same amounts, but only during 8 to 10, 22 to 24, and 36 to 38 days of life. Blood for analysis was collected from the wing vein of all chicks at the age of 42 days. After the rearing period (day 42), six birds from each experimental group with body weight similar to the group average were slaughtered. The carcasses were dissected and samples of the jejunum were collected for analysis of absorption of selected minerals. Mineral absorption was tested using the in vitro gastrointestinal sac technique. Oral administration of copper nanoparticles to chickens in the amount of 5, 10, and 15 mg/L led to accumulation of this element in the intestinal walls. The highest level of copper nanoparticles applied increased Cu content in the blood plasma of the birds. The in vitro study suggests that copper accumulated in the intestines reduces absorption of calcium and zinc, but does not affect iron absorption.

Effect of nanoparticles of silver on redox status and the accumulation of Ag in chicken tissues

“…However, it is known that intestinal lymphoid tissue (Peyer's patches) can absorb nanoparticles. [43][44][45] Intestinal fluid transported through the lymphatic vessels to the abdominal lymph nodes has been shown to play a significant role in the translocation of silver nanoparticles.…”

The effect of administration of copper nanoparticles to chickens in their drinking water on the immune and antioxidant status of the blood

“…Similarly, specialized proteins are involved in intracellular transport of Cu and incorporation of this element into enzyme molecules (Ognik et al . ).…”

Effects of copper nanoparticles on the morphological and physiological changes in chicken

“…Tương tự, thể trọng của gà, hàm lượng Cu có trong huyết tương (A) và gan (B) ở gà trong TN1 so với lô ĐC không có sự khác biệt (hình 2). Các nghiên cứu trước đây cũng đã chỉ ra khi cho gà uống nước chứa Cu có hàm lượng từ 5 đến 15 mg/L sẽ không gây ảnh hưởng tới nồng độ Cu có trong huyết tương gà (Ognik et al, 2016). Tuy nhiên, ở TN2, khi cho gà uống nước chứa nCu nồng độ 1.000 mg/L, hàm lượng Cu tích lũy trong huyết tương (A) và gan (B) tăng đáng kể so với nhóm đối chứng (P < 0,05), sự khác biệt này có ý nghĩa thống kê.…”