The Effect of Addition of Moringa Leaf Extract in Lactose Extender on Frozen Semen Quality of Boer Crossbreed Goat

Abstract

Moringa leaves contain various nutrients that can protect spermatozoa during semen cryopreservation. This study aimed to examine the effect of moringa leaf extract in tris extender on the quality of frozen semen of Boer crossbreed goat. Semen was collected with an artificial vagina. Fresh semen was divided into four tubes and diluted with 73% tris extender + 20% egg yolk + 7% glycerol (control), 71% tris extender + 20% egg yolk + 7% glycerol + 2% moringa leaf extract (MLE-2), 69% tris extender + 20% egg yolk + 7% glycerol + 4% moringa leaf extract (MLE-4), and 67% tris extender + 20% egg yolk + 7% glycerol + 6% moringa leaf extract (MLE-6), respectively. Diluted-semen was loaded in a mini straw and then stored in a liquid nitrogen container for seven days. The quality of spermatozoa including motility, viability, and intact plasma membrane (IPM) were observed after diluting and thawing. The result showed that the addition of moringa leaf extract into the tris extender could improve the spermatozoa quality of frozen semen of Boer crossbreed goat. At the stage after thawing, the percentage of motility, percentage of viability, and percentage of IPM of MLE-4 (52.2, 65.2, and 65.0%) were significantly higher (p<0.05) compared to the control (43.7, 53.5, and 53.7%). It can be concluded that the addition of 4% moringa leaf extract is the appropriate concentration in producing frozen semen of Boer crossbreed.