The Effect of Adding Blood on the Virulence Genes Expression of Staphylococcus aureus in Exponential and Stationary Growth Phase

Koohsari, Hadi; Ghaemi, Ezzat Allah; Mozaffari, N. Amir; Moradi, A; Sadegh-Sheshpoli, Maryam; Javid, Seyyed-Naemeh

doi:10.5812/jjm.14380

Cited by 4 publications

(3 citation statements)

References 26 publications

(21 reference statements)

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“…In contrast, Gram‐negative bacteria have a more complex cell wall structure, which includes an outer membrane composed of a double layer of phospholipids and lipopolysaccharides (Koohsari et al., 2015; Vikram et al., 2007). This outer membrane acts as a barrier, preventing the entry of many antimicrobial agents, including EOs.…”

Section: Resultsmentioning

confidence: 99%

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

Ezzaky,

Elmoslih,

Silva

et al. 2023

Comp Rev Food Sci Food Safe

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Essential oils (EOs) are a class of natural products that exhibit potent antimicrobial properties against a broad spectrum of bacteria. Inhibition diameters (IDs) and minimum inhibitory concentrations (MICs) are the typical measures of antimicrobial activity for extracts and EOs obtained from Cinnamomum, Salvia, and Mentha species. This study used a meta‐analytical regression analysis to investigate the correlation between ID and MIC measurements and the variability in antimicrobial susceptibility tests. By utilizing pooled ID models, this study revealed significant differences in foodborne pathogens’ susceptibility to extracts, which were dependent on both the plant species and the methodology employed (p < .05). Cassia showed the highest efficacy against Salmonella spp., exhibiting a pooled ID of 26.24 mm, while cinnamon demonstrated the highest efficacy against Bacillus cereus, with a pooled ID of 23.35 mm. Mint extract showed the greatest efficacy against Escherichia coli and Staphylococcus aureus. Interestingly, cinnamon extract demonstrated the lowest effect against Shiga toxin‐producing E. coli, with a pooled ID of only 8.07 mm, whereas its EOs were the most effective against this bacterial strain. The study found that plant species influenced the MIC, while the methodology did not affect MIC measurements (p > .05). An inverse correlation between ID and MIC measurements was identified (p < .0001). These findings suggest that extracts and EOs obtained from Cinnamomum, Salvia, and Mentha spp. have the potential to inhibit bacterial growth. The study highlights the importance of considering various factors that may influence ID and MIC measurements when assessing the effectiveness of antimicrobial agents.

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Section: Resultsmentioning

confidence: 99%

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

Ezzaky,

Elmoslih,

Silva

et al. 2023

Comp Rev Food Sci Food Safe

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“…Virulence genes can be classified into three types including (1) true virulence genes which encode factors or enzymes producing factors that are involved in bacteria-host interaction and directly cause the pathological damage during infection; (2) virulence-associated genes which encode factors or enzymes producing factors that control virulence gene expression or stimulate virulence factors or are essential for the activity of true virulence factors; and (3) virulence life-style genes which encode factors or enzymes producing factors that promote host colonization or enable evasion of the host immune system or enable interaction survival or employ host-factors for the advantages of survival [ 27 ]. Previous reports demonstrated that the expression of virulence genes was affected by several factors such as (1) nutrient starvation [ 28 , 29 , 30 ]; (2) osmotic agents (NaCl, sucrose, and glucose) [ 29 , 31 , 32 , 33 , 34 , 35 , 36 ]; (3) oxygen [ 37 ]; (4) temperature [ 31 , 38 ]; (5) growth phase [ 29 , 39 , 40 ]; (6) kind of carbon source [ 41 , 42 ]; (7) pH [ 43 ]; (8) sodium glycocholate [ 44 ]; and (9) L-glutamine [ 45 ]. Besides being a major problem in public health concern, enterobacteria are also highlighted for their application potential.…”

Section: Introductionmentioning

confidence: 99%

Identification, Characterization, and Virulence Gene Expression of Marine Enterobacteria in the Upper Gulf of Thailand

Nimnoi

Pongsilp

2022

Microorganisms

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Enterobacteria that commonly inhabit marine environments have a great impact on human health. In this study, enterobacteria isolated from seawater in the Upper Gulf of Thailand were identified and characterized. Seawater from nine sampling sites along the Upper Gulf of Thailand contained presumptive enterobacteria that ranged from 0.22 ± 0.44 to 17.00 ± 3.97 CFU/mL. The 101 strains belonged to seven species in which Klebsiella pneumoniae was the majority (47.5% of strains). The highest prevalence was resistant to ampicillin (76.2%) and ticarcillin (72.3%), respectively, whereas none was resistant to imipenem. Forty-five antibiotic resistance patterns were observed and 33.7% exhibited multidrug resistance, emphasizing the concern about public health. Three β-lactamase genes, including ampC, blaSHV, and blaTEM, were detected at the frequencies of 47.5%, 21.8%, and 11.9%, respectively. Six virulence genes, including csgD, uge, kfu, eaeA, magA, and LTI, were detected at the frequencies of 37.6%, 31.7%, 19.8%, 16.8%, 12.9%, and 5.9%, respectively. The condition of 4% NaCl downregulated the expression of the kfu and uge genes. The 67.3% and 63.4% of strains synthesized silver nanoparticles ranging between 3.04 ± 0.64 and 20.64 ± 0.95 μg/mL and gold nanoparticles ranging between 7.77 ± 0.45 and 57.57 ± 8.00 μg/mL, respectively.

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“…Virulence genes are consequently important for bacterial infection and pathogenesis. The expression of virulence genes has been found to be affected by several factors such as nutrient starvation (Chandra et al, 2017;Paytubi et al, 2017); concentrations of NaCl (Larsen & Jespersen, 2015), oxygen (Melson & Kendall, 2019), and glucose (Valdes et al, 2018); temperature (Guijarro et al, 2015); growth phase (Koohsari et al, 2017); kind of carbon source (Ferrando et al, 2014;Kentache et al, 2016); pH (Do et al, 2019); sodium glycocholate (Joffre et al, 2019); and L-glutamine (Haber et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Distribution and expression of virulence genes in potentially pathogenic bacteria isolated from seafood in Thailand

Nimnoi

Pongsilp

2020

CyTA - Journal of Food

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Since seafood can be a reservoir for diverse pathogenic bacteria, the risk to human health associated with seafood merits evaluation. The potentially pathogenic bacteria were isolated from seafood in Thailand. The 40 obtained strains were distinguished by ERIC-PCR fingerprints and identified to the species level by the VITEK 2 system. Their 16S rRNA gene had 97.74-99.79% nucleotide sequence identities to those of bacteria in 11 genera including Aeromonas, Citrobacter,

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The Effect of Adding Blood on the Virulence Genes Expression of Staphylococcus aureus in Exponential and Stationary Growth Phase

Cited by 4 publications

References 26 publications

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

Identification, Characterization, and Virulence Gene Expression of Marine Enterobacteria in the Upper Gulf of Thailand

Distribution and expression of virulence genes in potentially pathogenic bacteria isolated from seafood in Thailand

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