The Effect of Acth and Pathological Increases in Adrenal Cortical Function on Urinary Alphaketolic Steroid Metabolites 1

Dohan, F. C.; Touchstone, Joseph C.; Richardson, E.; Bulaschenko, Helen; Landolt, Irmgard; Applin, Walter J.

doi:10.1172/jci103098

Cited by 13 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although a considerable amount of work has been done in isolating and identifying such urinary metabolites of cortisol as THF8 and THE (17)(18)(19)(20)(21)(22) and their interconversions (23)(24)(25), much less has been done to relate the quantitative transformations of these urinary metabolites from cortisol under normal and pathological conditions. In 1953, deCourcy, Bush, Gray, and Lunnon (26) reported daily excretion values for two chromatographically separated urinary steroids corresponding in migration rates to THF and THE, in 10 1 This work was suported in part by a grant from the Atomic Energy Commission.…”

mentioning

confidence: 99%

Quantitative Determination of the Urinary Cortisolc Metabolites, “Tetrahydro F,” “Allo-Tetrahydro F” and “Tetrahydro E”: Effects of Adrenocorticotropin and Complex Trauma in the Human1

Gold¹,

Singleton²,

Macfarlane³

et al. 1958

J. Clin. Invest.

View full text Add to dashboard Cite

Methods for the quantitative estimation of plasma cortisol concentration have been devised by Nelson and Samuels (1), Silber and Porter (2), and Peterson and Wyngaarden (3) who used the sulfuric acid-induced fluorescence developed by Sweat (4). Together with urinary procedures (5, 6) these techniques have been employed to determine the secretion of cortisol by the adrenal in normal and pathological conditions (7-9) and to evaluate the factors involved in the "removal" of cortisol from the plasma in surgery (10-13) and liver disease (14-16).Although a considerable amount of work has been done in isolating and identifying such urinary metabolites of cortisol as THF8 and THE (17-22) and their interconversions (23-25), much less has been done to relate the quantitative transformations of these urinary metabolites from cortisol under normal and pathological conditions. In 1953, deCourcy, Bush, Gray, and Lunnon (26) reported daily excretion values for two chromatographically separated urinary steroids corresponding in migration rates to THF and THE, in 10 1 This work was suported in part by a grant from the Atomic Energy Commission. It was sponsored and supported in part by the Subcommittee on Metabolism in Trauma, Advisory Committee on Metabolism, Office of the Surgeon General, Department of the Army, through a contract (DA-49-MD472) with Harvard University.The assistance of Winthrop Laboratories, Inc., and The Upjohn Co. is gratefully acknowledged.2 Present address: The Medical College of St. Bartholomew's Hospital, London. 3 Compounds referred to are the following: THF, tetrahydrocortisol (3a,11p,17a,21-tetrahydroxypregnan-20-one ); allo-THF (3a,1 1,l17a,21-tetrahydroxyallopregnan-20-one); THE, tetrahydrocortisone (3a,17a,21-trihydroxypregnan-11,20-dione); F, cortisol (llfi,17a,21-trihydroxypregnan-3,20-dione); E, cortisone (17a,21-dihydroxypregnan-3,11,20-trione).normal men. The mean values were 212 tg. per 24 hours for THF and 1.5 mg. per 24 hours for THE, giving a ratio for THF: THE of 0.14. Romanoff, Seelye, Rodriquez, and Pincus (27) have recently published quantitative data on the excretion of THF and THE combined with allo-THF (28, 29) in one group of normal men and in a group of schizophrenic men. There was no essential difference in the ratio of THF:(THE plus allo-THF) between the two groups of men, and the ratio was reported as approximately 1: 2. Cope and Hurlock (30) published data on the excretion of urinary THF and THE in several normal and surgical cases. Their results indicated increased excretion of these metabolites after surgery but did not show any other definite pattern of alteration of the metabolism of cortisol, although they state that "a tendency can be observed for tetrahydro-compound F excretion to rise more than does tetrahydrocortisone." In a preliminary report, Gold, Macfarlane, and Moore (31) showed that the relative proportions of the two urinary cortisol metabolites, THF and THE, were related to situations of "stress" and adrenocorticotropin (ACTH) administration. It is the purpo...

show abstract

mentioning

confidence: 99%

Quantitative Determination of the Urinary Cortisolc Metabolites, “Tetrahydro F,” “Allo-Tetrahydro F” and “Tetrahydro E”: Effects of Adrenocorticotropin and Complex Trauma in the Human1

Gold¹,

Singleton²,

Macfarlane³

et al. 1958

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The reversal of the ratio of tetrahydro E:tetrahydro F in Cushing's syndrome and after ACTH has been amply discussed by Gold et al (1959). The occurrence of tetrahydro S and its increase in response to ACTH has been observed (Dohan et al 1955) and as with pregnanetriol probably represents only an overflow of their respective precursors during the synthesis of cortisol.…”

Section: Discussionmentioning

confidence: 99%

Urinary Steroids in Post-Natal Adrenal Hyperplasia With Virilism

Lipsett

Riter

1961

Acta Endocrinologica

View full text Add to dashboard Cite

S The urinary steroids have been analyzed in a 12-year-old girl with adrenal hyperplasia in whom virilization started at age 6. Androsterone and etiocholanolone levels were elevated and increased normally in response to adrenocorticotrophin (ACTH). The excretion of 11β-hydroxy-androsterone was high and of 11β-hydroxy and 11-ketoetiocholanolone low. She excreted 16 mg of pregnanetriol daily before ACTH, and 48 mg with ACTH. The excretion of tetrahydrocortisol and tetrahydrocortisone was normal and increased with ACTH. This steroid pattern was compared with that of 3 patients with adrenal hyperplasia and Cushing's syndrome. These patients excreted only slightly increased amounts of pregnanetriol and large amounts of all of the 11-oxyketosteroids. The occurrence of post-natal virilizing adrenal hyperplasia has been reviewed and the hypothesis offered that there is a congenital partial block of 21-hydroxylation that becomes clinically apparent only some years later.

show abstract

“…The adrenal cortex secretes many different hormones, and those that produce Cushing's syndrome belong to the glucocorticoid group having a hydroxyl group at the C-1 7 and usually a hydroxyl or ketone group at the C-lI positions. Large amounts of hydrocortisone were first isolated from a patient with Cushing's syndrome by Mason and Sprague (1948), and subsequently excess of cortisone and compound S (17-hydroxy-11desoxycorticosterone) has been found in the urine of other patients (Dohan et al, 1955). Theoretically excessive formation of corticosterone might cause Cushing's syndrome but no instance of this has yet been reported although increased amounts may arise in parallel with overproduction of hydrocortisone (Sweat, 1955).…”

Section: Section Of Endocrinologymentioning

confidence: 99%

Discussion on Cushing's Syndrome

1957

Proceedings of the Royal Society of Medicine

View full text Add to dashboard Cite

The Effect of Acth and Pathological Increases in Adrenal Cortical Function on Urinary Alphaketolic Steroid Metabolites 1

Cited by 13 publications

References 24 publications

Quantitative Determination of the Urinary Cortisolc Metabolites, “Tetrahydro F,” “Allo-Tetrahydro F” and “Tetrahydro E”: Effects of Adrenocorticotropin and Complex Trauma in the Human1

Quantitative Determination of the Urinary Cortisolc Metabolites, “Tetrahydro F,” “Allo-Tetrahydro F” and “Tetrahydro E”: Effects of Adrenocorticotropin and Complex Trauma in the Human1

Urinary Steroids in Post-Natal Adrenal Hyperplasia With Virilism

Discussion on Cushing's Syndrome

Contact Info

Product

Resources

About